LOCUS       sequence-137215-         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ETV5
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(397..417)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(442..459)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             470..492
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    507..531
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(531..550)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(532..550)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(634..653)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgca agcttgtcga
       61 cggagctcga attcggatcc gtatccacct ttactgtcag ttatccggga aagccatgga
      121 gaagagggca tctgggtcac agacaaattt gtagacgtat cgctctccag ccaccttctg
      181 catgatgccc ttttcatagt aatagcggag agagcggctc agcttgtcat agttcatggc
      241 tggccggttc ttctggatgc cccagcgccg agcaacctct tccggttcta tcagcttgaa
      301 ctccatgcct cgacctgtcc aggcaatgaa gtgggcattg gctgggtcat caagaagggt
      361 gaccaggaac tgccacagct gaagggaacc tcgcatggat tggaagtaca ggttctcggt
      421 acccagatct acaccagaag aatgatgatg atgatggtgc atatgtatat ctccttctta
      481 aagttaaaca aaattatttc tagaggggaa ttgttatccg ctcacaattc ccctatagtg
      541 agtcgtatta atttcgcggg atcgagatct cgatcctcta cgccggacgc atcgtggccg
      601 gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg
      661 aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag
      721 gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg
      781 cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcagga
//
