LOCUS       sequence-137223-         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MAPK3
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..43
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..88
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     regulatory      87..96
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg gtgtagatct
       61 gggtaccgag aacctgtact tccaatccat ggcggcggcg gcggctcagg ggggcggggg
      121 cggggagccc cgtagaaccg agggggtcgg cccgggggtc ccgggggagg tggagatggt
      181 gaaggggcag ccgttcgacg tgggcccgcg ctacacgcag ttgcagtaca tcggcgaggg
      241 cgcgtacggc atggtcagct cggcctatga ccacgtgcgc aagactcgcg tggccatcaa
      301 gaagatcagc cccttcgaac atcagaccta ctgccagcgc acgctccggg agatccagat
      361 cctgctgcgc ttccgccatg agaatgtcat cggcatccga gacattctgc gggcgtccac
      421 cctggaagcc atgagagatg tctacattgt gcaggacctg atggagactg acctgtacaa
      481 gttgctgaaa agccagcagc tgagcaatga ccatatctgc tacttcctct accagatcct
      541 gcggggcctc aagtacatcc actccgccaa cgtgctccac cgagatctaa agccctccaa
      601 cctgctcatc aacaccacct gcgaccttaa gatttgtgat ttcggcctgg cccggattgc
      661 cgatcctgag catgaccaca ccggcttcct gacggagtat gtggctacgc gctggtaccg
      721 ggccccagag atcatgctga actccaaggg ctataccaag tccatcgaca tctggtctgt
      781 gggctgcatt ctggctga
//
