LOCUS       sequence-137230-         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SPHK1
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             80..97
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             122..142
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ataaaaaaac ctataaatat tccggattat tcataccgtc ccaccatcgg gcgcggatct
       61 cggtccgaaa accatgggcc accatcatca tcatcattct tctggtgtag atctgggtac
      121 cgagaacctg tacttccaat ccatggagcc gcgggtcgag gttatggatc cagcgggcgg
      181 cccccggggc gtgctcccgc ggccctgccg cgtgctggtg ctgctgaacc cgcgcggtgg
      241 caagggcaag gccttgcagc tcttccggag tcacgtgcag ccccttttgg ctgaggctga
      301 aatctccttc acgctgatgc tcactgagcg gcggaaccac gcgcgggagc tggtgcggtc
      361 ggaggagctg ggccgctggg acgctctggt ggtcatgtct ggagacgggc tgatgcacga
      421 ggtggtgaac gggctcatgg agcggcctga ctgggagacc gccatccaga agcccctgtg
      481 tagcctccca gcaggctctg gcaacgcgct ggcagcttcc ttgaaccatt atgctggcta
      541 tgagcaggtc accaatgaag acctcctgac caactgcacg ctattgctgt gccgccggct
      601 gctgtcaccc atgaacctgc tgtctctgca cacggcttcg gggctgcgcc tcttctctgt
      661 gctcagcctg gcctggggct tcattgctga tgtggaccta gagagtgaga agtatcggcg
      721 tctgggggag atgcgcttca ctctgggcac cttcctgcgt ctggcagccc tgcgcaccta
      781 ccgcggccga ctggcctacc tccctgtagg aagagtgggt tccaagacac ctgcctcccc
      841 cgttgtggtc cagcagggcc cggtagatgc acaccttgtg ccactggagg a
//
