LOCUS       sequence-137238-        8414 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8414)
  AUTHORS   Subach FV, Patterson GH, Renz M, Lippincott-Schwartz J, Verkhusha VV
  TITLE     Bright monomeric photoactivatable red fluorescent protein for 
            two-color super-resolution sptPALM of live cells.
  JOURNAL   J Am Chem Soc. 2010 May 12;132(18):6481-91.
  PUBMED    20394363
REFERENCE   2  (bases 1 to 8414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc."; date: "2010-05-12"; volume: "132(18)"; pages: "6481-91"
COMMENT     Sequence Label: pEGFR-PATagRFP
FEATURES             Location/Qualifiers
     source          1..8414
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        68..371
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        372..575
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     526..546
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             join(725..796,797..2659,2660..2728,2729..4354)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     CDS             4385..5086
                     /codon_start=1
                     /product="photoactivatable variant of the monomeric red 
                     fluorescent protein TagRFP (Subach et al., 2010)"
                     /label=PA-TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSSTFINHTQGIPDFWKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMKKKTLGWEPSTEKLKPADGGLEGRVDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDRRLEIIKEADKETYWEQHEVAVARYS
                     DLPSKLGHLYK"
     polyA_signal    5209..5330
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5246..5265)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5300..5319
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5337..5792)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5474..5495)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5686..5705
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5819..5923
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5925..6282
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5925..6116
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5948..5968)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6133..6268
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6317..7111
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6317..7111
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6371..6390)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6981..7000
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7299..7318)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7343..7390
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7719..8307
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8208..8227
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
       61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      601 agagagctta tcgattctag ccgagtcccc gcctcgccgc caacgccaca accaccgcgc
      661 acggccccct gactccgtcc agtattgatc gggagagccg gagcgagctc ttcggggagc
      721 agcgatgcga ccctccggga cggccggggc agcgctcctg gcgctgctgg ctgcgctctg
      781 cccggcgagt cgggctctgg aggaaaagaa agtttgccaa ggcacgagta acaagctcac
      841 gcagttgggc acttttgaag atcattttct cagcctccag aggatgttca ataactgtga
      901 ggtggtcctt gggaatttgg aaattaccta tgtgcagagg aattatgatc tttccttctt
      961 aaagaccatc caggaggtgg ctggttatgt cctcattgcc ctcaacacag tggagcgaat
     1021 tcctttggaa aacctgcaga tcatcagagg aaatatgtac tacgaaaatt cctatgcctt
     1081 agcagtctta tctaactatg atgcaaataa aaccggactg aaggagctgc ccatgagaaa
     1141 tttacaggaa atcctgcatg gcgccgtgcg gttcagcaac aaccctgccc tgtgcaacgt
     1201 ggagagcatc cagtggcggg acatagtcag cagtgacttt ctcagcaaca tgtcgatgga
     1261 cttccagaac cacctgggca gctgccaaaa gtgtgatcca agctgtccca atgggagctg
     1321 ctggggtgca ggagaggaga actgccagaa actgaccaaa atcatctgtg cccagcagtg
     1381 ctccgggcgc tgccgtggca agtcccccag tgactgctgc cacaaccagt gtgctgcagg
     1441 ctgcacaggc ccccgggaga gcgactgcct ggtctgccgc aaattccgag acgaagccac
     1501 gtgcaaggac acctgccccc cactcatgct ctacaacccc accacgtacc agatggatgt
     1561 gaaccccgag ggcaaataca gctttggtgc cacctgcgtg aagaagtgtc cccgtaatta
     1621 tgtggtgaca gatcacggct cgtgcgtccg agcctgtggg gccgacagct atgagatgga
     1681 ggaagacggc gtccgcaagt gtaagaagtg cgaagggcct tgccgcaaag tgtgtaacgg
     1741 aataggtatt ggtgaattta aagactcact ctccataaat gctacgaata ttaaacactt
     1801 caaaaactgc acctccatca gtggcgatct ccacatcctg ccggtggcat ttaggggtga
     1861 ctccttcaca catactcctc ctctggatcc acaggaactg gatattctga aaaccgtaaa
     1921 ggaaatcaca gggtttttgc tgattcaggc ttggcctgaa aacaggacgg acctccatgc
     1981 ctttgagaac ctagaaatca tacgcggcag gaccaagcaa catggtcagt tttctcttgc
     2041 agtcgtcagc ctgaacataa catccttggg attacgctcc ctcaaggaga taagtgatgg
     2101 agatgtgata atttcaggaa acaaaaattt gtgctatgca aatacaataa actggaaaaa
     2161 actgtttggg acctccggtc agaaaaccaa aattataagc aacagaggtg aaaacagctg
     2221 caaggccaca ggccaggtct gccatgcctt gtgctccccc gagggctgct ggggcccgga
     2281 gcccagggac tgcgtctctt gccggaatgt cagccgaggc agggaatgcg tggacaagtg
     2341 caaccttctg gagggtgagc caagggagtt tgtggagaac tctgagtgca tacagtgcca
     2401 cccagagtgc ctgcctcagg ccatgaacat cacctgcaca ggacggggac cagacaactg
     2461 tatccagtgt gcccactaca ttgacggccc ccactgcgtc aagacctgcc cggcaggagt
     2521 catgggagaa aacaacaccc tggtctggaa gtacgcagac gccggccatg tgtgccacct
     2581 gtgccatcca aactgcacct acggatgcac tgggccaggt cttgaaggct gtccaacgaa
     2641 tgggcctaag atcccgtcca tcgccactgg gatggtgggg gccctcctct tgctgctggt
     2701 ggtggccctg gggatcggcc tcttcatgcg aaggcgccac atcgttcgga agcgcacgct
     2761 gcggaggctg ctgcaggaga gggagcttgt ggagcctctt acacccagtg gagaagctcc
     2821 caaccaagct ctcttgagga tcttgaagga aactgaattc aaaaagatca aagtgctggg
     2881 ctccggtgcg ttcggcacgg tgtataaggg actctggatc ccagaaggtg agaaagttaa
     2941 aattcccgtc gctatcaagg aattaagaga agcaacatct ccgaaagcca acaaggaaat
     3001 cctcgatgaa gcctacgtga tggccagcgt ggacaacccc cacgtgtgcc gcctgctggg
     3061 catctgcctc acctccaccg tgcaactcat cacgcagctc atgcccttcg gctgcctcct
     3121 ggactatgtc cgggaacaca aagacaatat tggctcccag tacctgctca actggtgtgt
     3181 gcagatcgca aagggcatga actacttgga ggaccgtcgc ttggtgcacc gcgacctggc
     3241 agccaggaac gtactggtga aaacaccgca gcatgtcaag atcacagatt ttgggctggc
     3301 caaactgctg ggtgcggaag agaaagaata ccatgcagaa ggaggcaaag tgcctatcaa
     3361 gtggatggca ttggaatcaa ttttacacag aatctatacc caccagagtg atgtctggag
     3421 ctacggggtg accgtttggg agttgatgac ctttggatcc aagccatatg acggaatccc
     3481 tgccagcgag atctcctcca tcctggagaa aggagaacgc ctccctcagc cacccatatg
     3541 taccatcgat gtctacatga tcatggtcaa gtgctggatg atagacgcag atagtcgccc
     3601 aaagttccgt gagttgatca tcgaattctc caaaatggcc cgagaccccc agcgctacct
     3661 tgtcattcag ggggatgaaa gaatgcattt gccaagtcct acagactcca acttctaccg
     3721 tgccctgatg gatgaagaag acatggacga cgtggtggat gccgacgagt acctcatccc
     3781 acagcagggc ttcttcagca gcccctccac gtcacggact cccctcctga gctctctgag
     3841 tgcaaccagc aacaattcca ccgtggcttg cattgataga aatgggctgc aaagctgtcc
     3901 catcaaggaa gacagcttct tgcagcgata cagctcagac cccacaggcg ccttgactga
     3961 ggacagcata gacgacacct tcctcccagt gcctgaatac ataaaccagt ccgttcccaa
     4021 aaggcccgct ggctctgtgc agaatcctgt ctatcacaat cagcctctga accccgcgcc
     4081 cagcagagac ccacactacc aggaccccca cagcactgca gtgggcaacc ccgagtatct
     4141 caacactgtc cagcccacct gtgtcaacag cacattcgac agccctgccc actgggccca
     4201 gaaaggcagc caccaaatta gcctggacaa ccctgactac cagcaggact tctttcccaa
     4261 ggaagccaag ccaaatggca tctttaaggg ctccacagct gaaaatgcag aatacctaag
     4321 ggtcgcgcca caaagcagtg aatttattgg agcaccgcgg gcccgggatc caccggtcgc
     4381 caccatgagc gagctgatta aggagaacat gcacatgaag ctctacatgg agggcaccgt
     4441 gaacaaccac cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca
     4501 gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc
     4561 taccagcttc atgtacggca gcagcacctt catcaaccac acccagggca tccccgactt
     4621 ctggaagcag tccttccctg agggcttcac atgggagaga gtcaccacat acgaagacgg
     4681 gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt
     4741 caagatcaga ggggtgaact tcccatccaa cggccctgtg atgaagaaga aaacactcgg
     4801 ctgggaaccg agcaccgaga aactgaaacc cgctgacggc ggcctggaag gcagggtcga
     4861 catggccctg aagctcgtgg gcgggggcca cctgatctgc aacttcaaga ccacatacag
     4921 atccaagaaa cccgctaaga acctcaagat gcccggcgtc tactatgtgg accgcagact
     4981 ggaaatcatc aaggaggccg acaaagagac ctactgggag cagcacgagg tggctgtggc
     5041 cagatactcc gacctcccta gcaaactggg gcacctgtac aagtaaagcg gccgcgactc
     5101 tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     5161 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     5221 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     5281 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat
     5341 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     5401 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     5461 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     5521 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     5581 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     5641 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     5701 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     5761 cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg
     5821 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     5881 ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac
     5941 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     6001 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     6061 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     6121 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     6181 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     6241 aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg
     6301 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     6361 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     6421 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     6481 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     6541 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     6601 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     6661 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     6721 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     6781 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     6841 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     6901 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     6961 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     7021 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     7081 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     7141 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     7201 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     7261 atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata
     7321 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg
     7381 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
     7441 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt
     7501 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca
     7561 ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     7621 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     7681 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     7741 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     7801 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     7861 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     7921 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     7981 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     8041 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     8101 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     8161 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     8221 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     8281 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     8341 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     8401 aaccgtatta ccgc
//
