LOCUS       sequence-137240-        9673 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of mutant G protein alpha 13 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9673)
  TITLE     Mariotti lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: psd44-G13QL
FEATURES             Location/Qualifiers
     source          1..9673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    55..76
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(91..96,97..114,115..121)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     mobile_element  122..889
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     protein_bind    906..922
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     911..933
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     930..946
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     930..946
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1012..1238
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1239..1419
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1466..1591
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2084..2317
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2502..2546
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2813..2930
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(2942..2962)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2947..3276
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3127..3262
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3286..3885
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3286..3305)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3782..3802
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     promoter        3984..4494
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4413..4431
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    5752..6340
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5805..5825)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6223..6234)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6427..6660
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6732..6853
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6769..6788)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6823..6842
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6893..7028
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7048..7067)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7049..7067)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7077..7094)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7077..7093)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7086..7108)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7235..7690
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7322..7341)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7532..7553
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7716..7820
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7821..7889,7890..8681)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8039..8058)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8852..9440
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9341..9360
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9594..9611
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
       61 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      121 gggtaatgac tccaacttat tgatagtgtt ttatgttcag ataatgcccg atgactttgt
      181 catgcagctc caccgatttt gagaacgaca gcgacttccg tcccagccgt gccaggtgct
      241 gcctcagatt caggttatgc cgctcaattc gctgcgtata tcgcttgctg attacgtgca
      301 gctttccctt caggcgggat tcatacagcg gccagccatc cgtcatccat atcaccacgt
      361 caaagggtga cagcaggctc ataagacgcc ccagcgtcgc catagtgcgt tcaccgaata
      421 cgtgcgcaac aaccgtcttc cggagactgt catacgcgta aaacagccag cgctggcgcg
      481 atttagcccc gacatagccc cactgttcgt ccatttccgc gcagacgatg acgtcactgc
      541 ccggctgtat gcgcgaggtt accgactgcg gcctgagttt tttaagtgac gtaaaatcgt
      601 gttgaggcca acgcccataa tgcgggctgt tgcccggcat ccaacgccat tcatggccat
      661 atcaatgatt ttctggtgcg taccgggttg agaagcggtg taagtgaact gcagttgcca
      721 tgttttacgg cagtgagagc agagatagcg ctgatgtccg gcggtgcttt tgccgttacg
      781 caccaccccg tcagtagctg aacaggaggg acagctgata gaaacagaag ccactggagc
      841 acctcaaaaa caccatcata cactaaatca gtaagttggc agcatcaccc gtatgttgtg
      901 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
      961 gcgcgcgttt aaactcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt
     1021 atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag
     1081 gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat
     1141 taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca
     1201 gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga
     1261 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     1321 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     1381 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     1441 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     1501 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     1561 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     1621 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     1681 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     1741 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     1801 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     1861 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     1921 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     1981 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     2041 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     2101 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     2161 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     2221 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     2281 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     2341 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     2401 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     2461 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     2521 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     2581 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     2641 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     2701 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     2761 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta acttttaaaa
     2821 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     2881 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt atcgaccctg
     2941 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     3001 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     3061 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     3121 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     3181 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     3241 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttaccatgac cgagtacaag
     3301 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     3361 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     3421 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     3481 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     3541 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     3601 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     3661 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     3721 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     3781 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     3841 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac
     3901 ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat cgatcacgag actagcctcg
     3961 agaagcttga tatcgaattc cacggggttg gggttgcgcc ttttccaagg cagccctggg
     4021 tttgcgcagg gacgcggctg ctctgggcgt ggttccggga aacgcagcgg cgccgaccct
     4081 gggtctcgca cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt
     4141 gtgggccccc cggcgacgct tcctgctccg cccctaagtc gggaaggttc cttgcggttc
     4201 gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca ctagtaccct cgcagacgga
     4261 cagcgccagg gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc
     4321 tcagcagggc gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg
     4381 gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg cattctgcaa gcctccggag
     4441 cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg acctctctcc ccagggggat
     4501 ccaccggtta cgtatctaga ttcgaacata tgttaattaa cctgcagggc tagcgtttaa
     4561 acttaagctt ggtaccacca tggcggactt cctgccgtcg cggtccgtgc tgtccgtgtg
     4621 cttccccggc tgcctgctga cgagtggcga ggccgagcag caacgcaagt ccaaggagat
     4681 cgacaaatgc ctgtctcggg aaaagaccta tgtgaagcgg ctggtgaaga tcctgctgct
     4741 gggcgcgggc gagagcggca agtccacctt cctgaagcag atgcggatca tccacgggca
     4801 ggacttcgac cagcgcgcgc gcgaggagtt ccgccccacc atctacagca acgtgatcaa
     4861 aggtatgagg gtgctggttg atgctcgaga gaagcttcat attccctggg gagacaactc
     4921 aaaccaacaa catggagata agatgatgtc gtttgatacc cgggccccca tggcagccca
     4981 aggaatggtg gaaacaaggg ttttcttaca atatcttcct gctataagag cattatgggc
     5041 agacagcggc atacagaatg cctatgaccg gcgtcgagaa tttcaactgg gtgaatctgt
     5101 aaaatatttc ctggataact tggataaact tggagaacca gattatattc catcacaaca
     5161 agatattctg cttgccagaa gacccaccaa aggcatccat gaatacgact ttgaaataaa
     5221 aaatgttcct ttcaaaatgg ttgatgtagg tggtctgaga tcagaaagga aacgttggtt
     5281 tgaatgtttc gacagtgtga catcaatact tttccttgtt tcctcaagtg aatttgacca
     5341 ggtgcttatg gaagatcgac tgaccaatcg ccttacagag tctctgaaca tttttgaaac
     5401 aatcgtcaat aaccgggttt tcagcaatgt ctccataatt ctgttcttaa acaagacaga
     5461 cttgcttgag gagaaggtgc aaattgtgag catcaaagac tatttcctag aatttgaagg
     5521 ggatccccac tgcttaagag acgtccaaaa attcctggtg gaatgtttcc ggaacaaacg
     5581 ccgggaccag caacagaagc ccttatacca ccacttcacc actgctatca acacggagaa
     5641 catccgcctt gttttccgtg acgtgaagga tactattctg catgacaacc tcaagcagct
     5701 tatgctacag tgatctagag atctctaggg gggccggcct gtacagtcga caatcaacct
     5761 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     5821 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     5881 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     5941 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     6001 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     6061 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     6121 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     6181 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     6241 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     6301 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattcg agctcggtac
     6361 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     6421 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     6481 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     6541 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     6601 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     6661 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     6721 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     6781 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     6841 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     6901 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     6961 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     7021 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     7081 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     7141 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     7201 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     7261 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     7321 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     7381 ctaaatcggg ggctcccttt agggttccga tttagtgcat tacggcacct cgaccccaaa
     7441 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     7501 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     7561 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     7621 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     7681 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     7741 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     7801 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     7861 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     7921 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     7981 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     8041 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     8101 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     8161 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     8221 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     8281 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     8341 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     8401 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     8461 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     8521 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     8581 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     8641 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     8701 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     8761 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     8821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     8881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     8941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     9001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     9061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     9121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     9181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     9241 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     9301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     9361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     9421 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     9481 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     9541 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     9601 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     9661 cgattcatta atg
//
