LOCUS       sequence-137245-        1067 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for expression of gRNA in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Li Y, Lin Z, Huang C, Zhang Y, Wang Z, Tang YJ, Chen T, Zhao X
  TITLE     Metabolic engineering of Escherichia coli using CRISPR-Cas9 
            meditated genome editing.
  JOURNAL   Metab Eng. 2015 Sep;31:13-21. doi: 10.1016/j.ymben.2015.06.006. Epub
            2015 Jun 30.
  PUBMED    26141150
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2015-09"; volume: "31:13-21. doi"; pages: " 
            10.1016/j.ymben.2015.06.006. Epub 2015 Jun 30"
COMMENT     Sequence Label: pGRB
FEATURES             Location/Qualifiers
     source          1..1067
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      239..827
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     728..747
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     981..998
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
       61 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
      121 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
      181 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
      241 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
      301 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
      361 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
      421 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
      481 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
      541 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
      601 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
      661 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
      721 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
      781 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
      841 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
      901 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
      961 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agggtgccac tttttcaagt
     1021 tgataacgga ctagccttat tttaacttgc tatttctagc tctaaaa
//
