LOCUS       sequence-137248-       11497 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11497)
  AUTHORS   Addis RC, Ifkovits JL, Pinto F, Kellam LD, Esteso P, Rentschler S, 
            Christoforou N, Epstein JA, Gearhart JD
  TITLE     Optimization of direct fibroblast reprogramming to cardiomyocytes 
            using calcium activity as a functional measure of success.
  JOURNAL   J Mol Cell Cardiol. 2013 Apr 13;60C:97-106. doi: 
            10.1016/j.yjmcc.2013.04.004.
  PUBMED    23591016
REFERENCE   2  (bases 1 to 11497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Cell 
            Cardiol."; date: "2013-04-13"; volume: "60C:97-106. doi"; pages: " 
            10.1016/j.yjmcc.2013.04.004"
COMMENT     Sequence Label: tetO-Myocd
FEATURES             Location/Qualifiers
     source          1..11497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(63..80)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(234..822)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(314..333)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(993..1784,1785..1853))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1616..1635
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1854..1958)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2033..2052)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2224..2603
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2605..2803
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             2821..3001
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3048..3173
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3666..3899
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4084..4128
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4426..4543
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4596..4614
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4638..4656
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4680..4698
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4722..4740
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4764..4782
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        4815..4853
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4850..4874
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     4953..4972
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4953..4971
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5003..5027
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8007..8031)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8057..8098
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             8108..8125
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    8175..8763
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8228..8248)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8646..8657)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8766..8782)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8767..8783)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9288..9468
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9494..9511)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9500..9724
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9770..10198
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9857..9876)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10067..10088
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10207..10227)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10212..10541
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10392..10527
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10454..10473
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10589..10636
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10655..11029
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11159..11280
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11196..11215)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11250..11269
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11329..11345)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11329..11345)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11342..11364)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11353..11369
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11377..11383,11384..11401,11402..11407))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11422..11443
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
       61 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      121 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      181 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      241 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      301 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      361 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      421 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      481 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      541 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      601 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      661 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      721 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      781 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      841 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      901 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
      961 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1021 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1081 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     1141 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     1201 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1261 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     1321 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     1381 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     1441 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     1501 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     1561 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     1621 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     1681 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     1741 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1801 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     1861 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     1921 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     1981 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
     2041 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
     2101 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
     2161 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
     2221 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     2281 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     2341 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     2401 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     2461 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     2521 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     2581 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     2641 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     2701 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     2761 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact
     2821 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     2881 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     2941 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     3001 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     3061 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     3121 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     3181 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     3241 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     3301 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     3361 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     3421 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     3481 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
     3541 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     3601 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     3661 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     3721 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     3781 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     3841 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     3901 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     3961 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     4021 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     4081 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     4141 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     4201 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     4261 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     4321 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     4381 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     4441 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     4501 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     4561 cagcagagat ccagtttggt tatcgagttt accactccct atcagtgata gagaaaagtg
     4621 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     4681 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     4741 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagctc
     4801 ggtacccggg tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag
     4861 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc
     4921 gggaccgatc cagcctccgc ggccccgaat tgtaatacga ctcactatag ggagacccaa
     4981 gctggctagg taagcttgat caacaagttt gtacaaaaaa gcagaagggc cgtcaaggcc
     5041 caccatgaca ctcctggggt ctgaacactc tttgctgatt agaaggaagt tccgatcagt
     5101 cttacagtta cggcttcaac agagaaggac ccaggagcag ctggctaacc aaggcttaat
     5161 accgccactg aaaggtccaa ctgaattcca tgacccgaga aaacaattgg atagtgccaa
     5221 gactgaagat tccctgaggc gcaagggcag aaacaggtcc gaccgtgcca gcctggttac
     5281 tatgcacatt ctccaagcct ccacggcaga aaggtccatt ccaactgctc agatgaagct
     5341 caaaagagcc cgccttgcag atgacctcaa tgagaagatc gctctccgcc cagggccctt
     5401 ggaactggtg gagaagaaca ttctgccgat ggattcttcc gtgaaagagg ctataaaagg
     5461 tactgaggtg agcctctcca aggcagcaga tgcattcgcc tttgaggatg acagcagtag
     5521 agatgggctc tctccagatc aggctaggag cgaggacccc cagggctcta caggatccac
     5581 cccagacatc aaatccactg aggctcctct ggacacaatc caggatctca ctcctggctc
     5641 agaaagtgac aagaatgatg cagcctccca gccaggcaac cagtcagacc ctgggaagca
     5701 ggttctcggc cccctcagca ccccgattcc tgtgcacact gctgtaaagt ccaagtcttt
     5761 gggtgacagt aagaaccgcc acaaaaagcc caaagacccc aaaccaaagg tgaagaagct
     5821 caaataccat cagtacatcc ccccagacca gaaggcagag aagtctcccc cacccatgga
     5881 ctctgcctat gcccggctgc tccagcaaca gcagctattc ctgcagctac agatcctcag
     5941 ccagcagcag caacagcagc agcaacagca gcagcagcaa cagcagcagc agcagcagca
     6001 gcagcggttc agctaccctg ggatgcacca aacacacctc aaagaaccaa atgaacagat
     6061 ggccagaaat ccgaatcctt cttcaacacc actgagcaat acccctctat cccctgtcaa
     6121 aaatagcatt tctggacaaa ctggtgtttc ttctctcaaa ccaggccccc tcccacccaa
     6181 cctggatgat ctcaaggtgt cagagttaag acaacagctt cgaatccggg gcttgccagt
     6241 gtcaggcacc aagacagcgc tggtggaccg gcttcgtccc ttccaggatt gtgctggcaa
     6301 ccctgtgccc aactttgggg acatcacaac tgtcaccttt cctgtcacgc ccaacacctt
     6361 gcccagttat cagtcctccc cgacaggctt ctaccacttt ggcagcacaa gctccagccc
     6421 acccatctcc cccgcctcat ctgacttgtc cgctgcaggg tccctgccag acaccttcac
     6481 cgatgcgtca cctggcttcg gcctgcacgc atctccggtg cccgcctgca cggacgagag
     6541 tctgctgagc agcctgaatg ggggctcggg cccctccgag cctgatgggc tagactctga
     6601 gaaggacaag atgctggtgg agaagcagaa agtgatcaac cagctcacct ggaagctgcg
     6661 gcaagagcag cggcaggtgg aagagctgag aatgcaactg cagaagcaga agagcagctg
     6721 cagcgaccag aagccactgc ccttcttggc caccaccatc aaacaggaag atgtctccag
     6781 ctgccccttc gcaccccagc aggcgtctgg gaagggacag ggccacagct ctgacagtcc
     6841 ccctccggct tgtgagacgg ctcagctgct gcctcactgt gtggagtcct caggtcaaac
     6901 ccatgtactc tcgtccacgt ttctcagccc ccagtgctcc cctcagcact cgcccctggg
     6961 gggcctgaag agcccgcagc acatcagcct gcctccatca cccaacaacc attacttcct
     7021 ggcttcctct tcgggagctc agagagagaa ccatggggtc tcttcaccca gcagcagcca
     7081 agggtgcgca cagaactcag gggcacacga aggccattct tctagcttct cttccccagc
     7141 ttccagcctc catcagcctt tctctggcac ccaagcagac agcagtcaca gtgctgggct
     7201 caacccttgt cccaaaagcc caagtattca tccaaagatg actggtttac aatcttctga
     7261 caaggtgggg ccaacgtttt caattccatc cccaactttt tctaagtcaa gttcagcagt
     7321 ttcagatatc acccagcccc catcctatga agatgcagtg aagcagcaaa tgactcggag
     7381 tcagcagatg gacgaactcc tggatgtcct cattgaaagt ggagaaatgc cagccgatgc
     7441 cagggaagat cattcatgtc ttcagaaaat tccaaagatc cctgggtcct cctgcagccc
     7501 aactgccatc cccccgaagc cctcggcttc ctttgagcag gcatcttcgg gaggccagat
     7561 ggccttcgat cactacgcca acgacagtga cgaacacctg gaagtcttat tgaattctca
     7621 cagccccatc ggaaaggtga gcgatgttac cctcctcaaa atcggaagcg aggagcctcc
     7681 ttttgacagc atcatggatg gcttcccagg gaaggctgcg gaagatctct tcagtgctca
     7741 cgagctcttg cctgggcccc tctccccgat gcatgcacag ttgtcacctc cttctgtgga
     7801 cagcagtggt ctgcagctga gcttcacgga atctccttgg gaaacaatgg aatggctgga
     7861 cctcactcca cctagttcca cgccaggctt cagcaacctt acctccagtg ggcccagcat
     7921 tttcaacatc gattttctgg atgttacaga tcttaatctg aattccccta tggatctcca
     7981 cttacagcag tggtagggcc tcatgggccc agctttcttg tacaaagtgg ttgatctaga
     8041 gggcccgcgg ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg
     8101 taccggtcat catcaccatc accattgagt ttaaacccgc tgatcagcct caattcgata
     8161 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     8221 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     8281 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     8341 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     8401 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     8461 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     8521 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     8581 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     8641 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     8701 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     8761 cgcatcgata ccgtcgacct cgagacctag aaaaacatgg agcaatcaca agtagcaata
     8821 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
     8881 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
     8941 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     9001 atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca
     9061 caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag
     9121 ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg
     9181 tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc
     9241 gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt
     9301 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     9361 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     9421 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa
     9481 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     9541 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     9601 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     9661 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     9721 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     9781 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     9841 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     9901 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     9961 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
    10021 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
    10081 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
    10141 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
    10201 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
    10261 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
    10321 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
    10381 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
    10441 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
    10501 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
    10561 tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg
    10621 catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc
    10681 ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt
    10741 ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt
    10801 catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg
    10861 cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc
    10921 ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg
    10981 cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg
    11041 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
    11101 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
    11161 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
    11221 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
    11281 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
    11341 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
    11401 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
    11461 gcccgctttc cagtcgggaa acctgtcgtg ccagctg
//
