LOCUS       sequence-137251-         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of SnoopCatcher, an engineered protein forming 
            a spontaneous covalent bond with its cognate peptide partner 
            SnoopTag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Veggiani G, Nakamura T, Brenner MD, Gayet RV, Yan J, Robinson CV, 
            Howarth M
  TITLE     Programmable polyproteams built using twin peptide superglues.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201519214.
  PUBMED    26787909
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2016 Jan 19. pii: 201519214."
COMMENT     Sequence Label: pET28a-SnoopCatcher
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..78
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             85..420
                     /label=SnoopCatcher
     primer_bind     complement(518..536)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      532..579
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(703..722)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc
       61 ctggtgccgc gcggcagcca tatgaagccg ctgcgtggtg ccgtgtttag cctgcagaaa
      121 cagcatcccg actatcccga tatctatggc gcgattgatc agaatgggac ctatcaaaat
      181 gtgcgtaccg gcgaagatgg taaactgacc tttaagaatc tgagcgatgg caaatatcgc
      241 ctgtttgaaa atagcgaacc cgctggctat aaaccggtgc agaataagcc gattgtggcg
      301 tttcagattg tgaatggcga agtgcgtgat gtgaccagca ttgtgccgca ggatattccg
      361 gctacatatg aatttaccaa cggtaaacat tatatcacca atgaaccgat accgccgaaa
      421 taataaaagc ttgcggccgc actcgagcac taccaccacc accactgaga tccggctgct
      481 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa
      541 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc
      601 ggattggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
      661 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
      721 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg ggg
//
