LOCUS       sequence-137252-         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of SnoopCatcher, an engineered protein forming 
            a spontaneous covalent bond with its cognate peptide partner 
            SnoopTag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Veggiani G, Nakamura T, Brenner MD, Gayet RV, Yan J, Robinson CV, 
            Howarth M
  TITLE     Programmable polyproteams built using twin peptide superglues.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201519214.
  PUBMED    26787909
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2016 Jan 19. pii: 201519214."
COMMENT     Sequence Label: pET28a-SnoopCatcher
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(62..397)
                     /label=SnoopCatcher
     CDS             complement(404..421)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(431..448)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             467..489
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    504..528
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(528..547)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(529..547)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(631..650)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        856..933
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt agtgctcgag tgcggccgca agcttttatt
       61 atttcggcgg tatcggttca ttggtgatat aatgtttacc gttggtaaat tcatatgtag
      121 ccggaatatc ctgcggcaca atgctggtca catcacgcac ttcgccattc acaatctgaa
      181 acgccacaat cggcttattc tgcaccggtt tatagccagc gggttcgcta ttttcaaaca
      241 ggcgatattt gccatcgctc agattcttaa aggtcagttt accatcttcg ccggtacgca
      301 cattttgata ggtcccattc tgatcaatcg cgccatagat atcgggatag tcgggatgct
      361 gtttctgcag gctaaacacg gcaccacgca gcggcttcat atggctgccg cgcggcacca
      421 ggccgctgct gtgatgatga tgatgatggc tgctgcccat ggtatatctc cttcttaaag
      481 ttaaacaaaa ttatttctag aggggaattg ttatccgctc acaattcccc tatagtgagt
      541 cgtattaatt tcgcgggatc gagatctcga tcctctacgc cggacgcatc gtggccggca
      601 tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag
      661 atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc
      721 ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg
      781 tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag
      841 agcgtcgaga tcccggacac catcgaatgg cgcaaaacct ttcgcggtat ggcatgatag
      901 cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt atac
//
