LOCUS       sequence-137260-         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of tandem high affinity maltose binding protein
            domains linked to SpyCatcher, for solid-phase polyprotein 
            synthesis..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Veggiani G, Nakamura T, Brenner MD, Gayet RV, Yan J, Robinson CV, 
            Howarth M
  TITLE     Programmable polyproteams built using twin peptide superglues.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201519214.
  PUBMED    26787909
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2016 Jan 19. pii: 201519214."
COMMENT     Sequence Label: pET21 MBPx-SpyCatcher
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             62..79
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 actttaagaa ggagatatac atatgggcag cagccatcat catcatcatc acagcagcgg
       61 cctggtgccg cgcggcagcc atatggctag catgaaaatc gaagaaggta aactggtaat
      121 ctggattaac ggcgataaag gctataacgg tctcgctgaa gtcggtaaga aattcgagaa
      181 agataccgga attaaagtca ccgttgagca tccggataaa ctggaagaga aattcccaca
      241 ggttgcggca actggcgatg gccctgacat tatcttctgg gcacacgacc gctttggtgg
      301 ctacgctcaa tctggcctgt tggctgaaat caccccggac aaagcgttcc aggacaagct
      361 gtatccgttt acctgggatg ccgtacgtta caacggcaag ctgattgctt acccgatcgc
      421 tgttgaagcg ttatcgctga tttataacaa agatctgctg ccgaacccgc caaaaacctg
      481 ggaagagatc ccggcgctgg ataaagaact gaaagcgaaa ggtaagagcg cgctgatgtt
      541 caacctgcaa gaaccgtact tcacctggcc gctgattgct gctgacgggg gttatgcgtt
      601 caagtatggc gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac
      661 cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc
      721 agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc
      781 caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca
      841 accatccaaa ccgttcgttg gcgtgctgag cgca
//
