LOCUS       sequence-137264-         658 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector for Gateway based on vector #394 from the 
            Tol2Kit, containing a gRNA scaffold under the control of a zebrafish
            U6 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Ablain J, Durand EM, Yang S, Zhou Y, Zon LI
  TITLE     A CRISPR/Cas9 Vector System for Tissue-Specific Gene Disruption in 
            Zebrafish.
  JOURNAL   Dev Cell. 2015 Mar 4. pii: S1534-5807(15)00075-1. doi: 
            10.1016/j.devcel.2015.01.032.
  PUBMED    25752963
REFERENCE   2  (bases 1 to 658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2015 Mar 4. pii: S1534-5807(15)00075-1. doi: 
            10.1016/j.devcel.2015.01.032."
COMMENT     Sequence Label: pDestTol2pA2-U6:gRNA
FEATURES             Location/Qualifiers
     source          1..658
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggatcaccat ccgtcgcccg ggcgtgtcaa taatatcact ctgtacatcc acaaacagac
       61 gataacggct ctctctttta taggtgtaaa ccttaaactg catttcacca gcccctgttc
      121 tcgtcggcaa aggagccgtt catttcaata aaccgggcga cctcagccat cccttcctga
      181 ttttccgctt tccagcgttc ggcacgcaga cgacgggctt cattctgcat ggttgtgctt
      241 accgaaccgg agatattgac atcatatatg ccttgagcaa ctgatagctg tcgctgtcaa
      301 ctgtcactgt aatacgctgc ttcatagcat acctcttttt gacatacttc gggtatacat
      361 atcagtatat attcttatac cgcaaaaatc agcgcgcaaa tacgcatact gttatctggc
      421 ttttagtaag ccggatcctc tagattacgc cccgcctgcc actcatcgca gtactgttgt
      481 aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc
      541 gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg
      601 gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccagg
//
