LOCUS       sequence-137268-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of SpyCatcher linked to SnoopCatcher..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Veggiani G, Nakamura T, Brenner MD, Gayet RV, Yan J, Robinson CV, 
            Howarth M
  TITLE     Programmable polyproteams built using twin peptide superglues.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201519214.
  PUBMED    26787909
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2016 Jan 19. pii: 201519214."
COMMENT     Sequence Label: pET28a SpyCatcher-SnoopCatcher
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             455..790
                     /label=SnoopCatcher
     CDS             803..820
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 actttaagaa ggagatatac atatgtcgta ctaccatcac catcaccatc acgattacga
       61 tagtgctacc catattaaat tctcaaaacg tgatgaggac ggcaaagagt tagctggtgc
      121 aactatggag ttgcgtgatt catctggtaa aactattagt acatggattt cagatggaca
      181 agtgaaagat ttctacctgt atccaggaaa atatacattt gtcgaaaccg cagcaccaga
      241 cggttatgag gtagcaactg ctattacctt tacagttaat gagcaaggtc aggttactgt
      301 aaatggcaaa gcaactaaag gtgacgctca tattggatcc cccgccaacc tgaaggccct
      361 ggaggcccag aagcagaagg agcagagaca ggccgccgag gagctggcca acgccaagaa
      421 gctgaaggag cagctggaga agggatccca tatgaagccg ctgcgtggtg ccgtgtttag
      481 cctgcagaaa cagcatcccg actatcccga tatctatggc gcgattgatc agaatgggac
      541 ctatcaaaat gtgcgtaccg gcgaagatgg taaactgacc tttaagaatc tgagcgatgg
      601 caaatatcgc ctgtttgaaa atagcgaacc cgctggctat aaaccggtgc agaataagcc
      661 gattgtggcg tttcagattg tgaatggcga agtgcgtgat gtgaccagca ttgtgccgca
      721 ggatattccg gctacatatg aatttaccaa cggtaaacat tatatcacca atgaaccgat
      781 accgccgaaa taataactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa
      841 agcccgaaag gaagctga
//
