LOCUS       sequence-137270-         670 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of SpyCatcher linked to SnoopCatcher..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Veggiani G, Nakamura T, Brenner MD, Gayet RV, Yan J, Robinson CV, 
            Howarth M
  TITLE     Programmable polyproteams built using twin peptide superglues.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201519214.
  PUBMED    26787909
REFERENCE   2  (bases 1 to 670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2016 Jan 19. pii: 201519214."
COMMENT     Sequence Label: pET28a SpyCatcher-SnoopCatcher
FEATURES             Location/Qualifiers
     source          1..670
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(556..573)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             593..615
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    630..654
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 atatttgcca tcgctcagat tcttaaaggt cagtttacca tcttcgccgg tacgcacatt
       61 ttgataggtc ccattctgat caatcgcgcc atagatatcg ggatagtcgg gatgctgttt
      121 ctgcaggcta aacacggcac cacgcagcgg cttcatatgg gatcccttct ccagctgctc
      181 cttcagcttc ttggcgttgg ccagctcctc ggcggcctgt ctctgctcct tctgcttctg
      241 ggcctccagg gccttcaggt tggcggggga tccaatatga gcgtcacctt tagttgcttt
      301 gccatttaca gtaacctgac cttgctcatt aactgtaaag gtaatagcag ttgctacctc
      361 ataaccgtct ggtgctgcgg tttcgacaaa tgtatatttt cctggataca ggtagaaatc
      421 tttcacttgt ccatctgaaa tccatgtact aatagtttta ccagatgaat cacgcaactc
      481 catagttgca ccagctaact ctttgccgtc ctcatcacgt tttgagaatt taatatgggt
      541 agcactatcg taatcgtgat ggtgatggtg atggtagtac gacatatgta tatctccttc
      601 ttaaagttaa acaaaattat ttctagaggg gaattgttat ccgctcacaa ttcccctata
      661 gtgagtcgta
//
