LOCUS       sequence-137274-         662 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses RAJ31 riboregulator encased in a permuted intron exon 
            ribozyme, which circularises the riboregulator..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Rostain W, Shen S, Cordero T, Rodrigo G, Jaramillo A
  TITLE     Engineering a Circular Riboregulator in Escherichia coli.
  JOURNAL   Biodes Res. 2020 Sep 12;2020:1916789. doi: 10.34133/2020/1916789. 
            eCollection 2020.
  PUBMED    37849901
REFERENCE   2  (bases 1 to 662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biodes 
            Res."; date: "2020-09-12"; volume: "2020:1916789. doi"; pages: " 
            10.34133/2020/1916789. eCollection 2020"
COMMENT     Sequence Label: circRAJ31
FEATURES             Location/Qualifiers
     source          1..662
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(66..97)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     primer_bind     145..161
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     145..161
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc
       61 gatcaagtca aaagcctccg gtcggaggct tttgactttc tgctatggag gtcaggtatg
      121 atttaaatgg tcagtattga gcctcaggaa acagctatga catcaagctg actagataat
      181 ctagctgatc gtggaccgat catacgtata atgccgtaag atcacgggtc ggacgacagc
      241 tcgctcgtcc agaactgatc cagtcgcgct gctctcgtcg atccctgcag aaaaaaaatc
      301 cttagctttc gctaaggatc aattgagtcc tacaatttag cacgggattg tctactagag
      361 aggttccccg tttagataga ttacaagtat aagtcacctt atactcaggc ctcaattaac
      421 ccaagaaaac atgctagcag accaatacag tgacagcgga tagggactta attgcggatg
      481 agacgacggt aaagcttaat attgatcccc taggaatatt aaacggtagc attatgttca
      541 gataaggtcg ttaatcttac cccggaatta tatccagctg catgtcacca tgcagagcag
      601 actatatctc caacttgtta aagcaagttg tctatcgttt cgagtcactt gaccctactc
      661 cc
//
