LOCUS       sequence-137290-         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of myc-tagged mRAP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Sfeir A, Kabir S, van Overbeek M, Celli GB, de Lange T
  TITLE     Loss of Rap1 induces telomere recombination in the absence of NHEJ 
            or a DNA damage signal.
  JOURNAL   Science. 2010 Mar 26;327(5973):1657-61. doi: 
            10.1126/science.1185100.
  PUBMED    20339076
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2010-03-26- 26"; volume: "327"; 
            issue: "5973"; pages: "1657-61"
COMMENT     Sequence Label: pLPC myc-mRAP1
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      86..95
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             95..124
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accgagctcg gatcagccac catggaacaa aagttgattt ctgaagaaga
      121 tttgttaaga aagagaggat ccgcggaggc gatggatctg ggtaaagacc ccaatgggcc
      181 cactcactcc tccactctgt tcgtgagaga agacggcagc gccatgtcgt tttacgtgcg
      241 gcccagctcg gccaagcgcc ggctgtcgac gctcatcctg cacggcggcg gcaccgtgtg
      301 tcgggtgcag gaacccggag ccgtgcttct cgcccagccc ggggaggcgc tggccgaggc
      361 ttcgggcgac ttcatctcca cgcagtacat cctagactgc gtggatcgca acgagaagct
      421 ggacctggag gcctatcggc tgggcctgac ggagcaggcg tccgatccga agcccggggc
      481 ttccaccgag ggctccacgg aaccggagcc gcagcccctg accgggcgca tcgcctacac
      541 cgacgcggaa gatgtggcca tcctgaccta cgtgaaggag aacgcccgtt cgcccagctc
      601 ggtcacaggc aatgccttgt ggaaagcgat ggagaagagc tcgctcacgc agcactcctg
      661 gcagtcgctc aaggaccgct acctcaagca cctacggggt caggagcaca agtacctgct
      721 cgggaacgcc ccggtcagcc cgtcctccca gaagctcaaa cggaaggcgg agcaggaccc
      781 cgaggccgcg gatagcggag agccacagaa caagagagcg ccagacttgc ctga
//
