LOCUS       sequence-137298-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human IL23p19 in mammalian cells (V5 tag).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Ramnath D, Tunny K, Hohenhaus DM, Pitts CM, Bergot AS, Hogarth PM, 
            Hamilton JA, Kapetanovic R, Sturm RA, Scholz GM, Sweet MJ
  TITLE     TLR3 drives IRF6-dependent IL-23p19 expression and p19/EBI3 
            heterodimer formation in keratinocytes.
  JOURNAL   Immunol Cell Biol. 2015 Oct;93(9):771-9. doi: 10.1038/icb.2015.77. 
            Epub 2015 Aug 25.
  PUBMED    26303210
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/icb.2015.77"; journalName: "Immunol Cell Biol"; date: 
            "2015-10"; volume: "93"; issue: "9"; pages: "771-9"
COMMENT     Sequence Label: hIL23p19 pEF6-V5 (MJS_004)
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..29
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10..28
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             737..778
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             788..805
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcttggtact aatacgactc actataggga gacccaagct ggctaggtaa gcttggtacc
       61 gagctcggat ccactagtcc agtgtggtgg aattgccctt aagatgctgg ggagcagagc
      121 tgtaatgctg ctgttgctgc tgccctggac agctcagggc agagctgtgc ctgggggcag
      181 cagccctgcc tggactcagt gccagcagct ttcacagaag ctctgcacac tggcctggag
      241 tgcacatcca ctagtgggac acatggatct aagagaagag ggagatgaag agactacaaa
      301 tgatgttccc catatccagt gtggagatgg ctgtgacccc caaggactca gggacaacag
      361 tcagttctgc ttgcaaagga tccaccaggg tctgattttt tatgagaagc tgctaggatc
      421 ggatattttc acaggggagc cttctctgct ccctgatagc cctgtgggcc agcttcatgc
      481 ctccctactg ggcctcagcc aactcctgca gcctgagggt caccactggg agactcagca
      541 gattccaagc ctcagtccca gccagccatg gcagcgtctc cttctccgct tcaaaatcct
      601 tcgcagcctc caggcctttg tggctgtagc cgcccgggtc tttgcccatg gagcagcaac
      661 cctgagtccc aagggcaatt ctgcagatat ccagcacagt ggcggccgct cgagtctaga
      721 gggcccgcgg ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg
      781 taccggtcat catcaccatc accattgagt t
//
