LOCUS       sequence-137320-         655 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual-promoter plasmid to express knockdown-proof human TACC3 (L566A 
            and L567A substitutions) tagged with EGFP along with shRNA to knock 
            down endogenous TACC3 in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Gutierrez-Caballero C, Burgess SG, Bayliss R, Royle SJ
  TITLE     TACC3-ch-TOG track the growing tips of microtubules independently of
            clathrin and Aurora-A phosphorylation.
  JOURNAL   Biol Open. 2015 Jan 16;4(2):170-9. doi: 10.1242/bio.201410843.
  PUBMED    25596274
REFERENCE   2  (bases 1 to 655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/bio"; 
            journalName: "Biol Open"; date: "2015-01-16- 16"; volume: "4"; 
            issue: "2"; pages: "170-9"
COMMENT     Sequence Label: pBrain-GFP-TACC3KDP(L566,567A)-shTACC3
FEATURES             Location/Qualifiers
     source          1..655
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        18..232
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     153..172
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(305..321)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(306..322)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(350..368)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(389..405)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(389..405)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(402..424)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    413..429
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(437..443,444..461,462..467))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    482..503
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ccgggctgca ggaattcgaa cgctgacgtc atcaacccgc tccaaggaat cgcgggccca
       61 gtgtcactag gcgggaacac ccagcgcgcg tgcgccctgg caggaagatg gctgtgaggg
      121 acaggggagt ggcgccctgc aatatttgca tgtcgctatg tgttctggga aatcaccata
      181 aacgtgaaat gtctttggat ttgggaatct tataagttct gtatgagacc acagatcccc
      241 gttaccggaa gatcgtctgt tcaagagaca gacgatcttc cggtaacttt ttggaaaagc
      301 ttatcgatac cgtcgacctc gagggggggc ccggtaccag cttttgttcc ctttagtgag
      361 ggttaatttc gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
      421 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
      481 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
      541 acctgtcgtg ccagatagta atcaattacg gggtcattag ttcatagccc atatatggag
      601 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacc
//
