LOCUS       sequence-137325-         523 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vectors for the salycilate cascade expression system 
            (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm-T7 SD
            sequence-MCSII-sspH2 signal peptide, M13 replication origin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Medina C, Camacho EM, Flores A, Mesa-Pereira B, Santero E
  TITLE     Improved expression systems for regulated expression in Salmonella 
            infecting eukaryotic cells.
  JOURNAL   PLoS One. 2011;6(8):e23055. doi: 10.1371/journal.pone.0023055. Epub 
            2011 Aug 1.
  PUBMED    21829692
REFERENCE   2  (bases 1 to 523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(8):e23055. doi"; pages: " 
            10.1371/journal.pone.0023055. Epub 2011 Aug 1"
COMMENT     Sequence Label: pMPO 56
FEATURES             Location/Qualifiers
     source          1..523
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             371..393
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 atggctatct ctagaaaggc ctacccctta ggctttatgc aaccgaaaca ataataatgg
       61 agtcatgacc atgacaatgc acctggggct cgactatata gatagtctcg ttgaagaaga
      121 tgagaacgag ggcatctacc gctgcaagcg cgagatgttc accgaccctc ggctgttcga
      181 tttagagatg aaacacatct ttgagggcaa ctggatttat ctcgcccacg agagccagat
      241 tcccgagaag aacgactatt acaccacgca gatgggccgg cagccgatat tcatcacacg
      301 caacaaagat ggtgagctga atgccttcgt caatgcctga attcccaatt cggttcaccc
      361 gggaaataat tttgtttaac tttaagaagg agatatacat atgccctttc atattggaag
      421 cggatgtctt cccgccacca tcagtaatcg ccgcatttat cgtattgcct ggtctgatac
      481 cccccctgaa atgagttcct gggaaaaaat gaaggaattt ttt
//
