LOCUS       sequence-137327-        6862 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of myc-tagged mRAP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6862)
  AUTHORS   Sfeir A, Kabir S, van Overbeek M, Celli GB, de Lange T
  TITLE     Loss of Rap1 induces telomere recombination in the absence of NHEJ 
            or a DNA damage signal.
  JOURNAL   Science. 2010 Mar 26;327(5973):1657-61. doi: 
            10.1126/science.1185100.
  PUBMED    20339076
REFERENCE   2  (bases 1 to 6862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2010-03-26- 26"; volume: "327"; 
            issue: "5973"; pages: "1657-61"
COMMENT     Sequence Label: pLPC myc-mRAP1
FEATURES             Location/Qualifiers
     source          1..6862
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             120..589
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(1974..2003)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      2003..2012
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2043..2062)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2044..2062)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2107..2310)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2136..2156)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2311..2690
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     2862..2881
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(2970..3569)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3053..3073)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     3550..3569
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(3587..4003)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(3603..3619)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(3635..3657)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    4204..4403
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             4468..4937
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        4968..5072
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5073..5141,5142..5933)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5291..5310)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6093..6681
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6582..6601
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6835..6852
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaactatg
       61 gccatttaat gtaaatactt aagaaaaaaa accaaattaa ttttgataca tgctgcatgt
      121 gaaagacccc cgctgacggg tagtcaatca ctcagaggag accctcccaa ggaacagcga
      181 gaccacaagt cggatgcaac tgcaagaggg tttattggat acacgggtac ccgggcgact
      241 cagtcaatcg gaggactggc gccccgagtg aggggttgtg ggctctttta ttgagctcgg
      301 ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca
      361 cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagaaactgc
      421 tgagggctgg accgcatctg gggaccatct gttcttggcc ctgagccggg gcaggaactg
      481 cttaccacag atatcctgtt tggcccatat tcagctgttc catctgttct tggccctgag
      541 ccggggcagg aactgcttac cacagatatc ctgtttggcc catattcagc tgttccatct
      601 gttcctgacc ttgatctgaa cttctctatt ctcagttatg tatttttcca tgccttgcaa
      661 aatggcgtta cttaagctag cttgccaaac ctacaggtgg ggtctttcat tccccccttt
      721 ttctggagac taaataaaat cttttatttt atcggccctc tagatgcatg ctcgagttat
      781 ttctttcgga attcaatcct ccgagcaacg ttctgagctc caaacttttt gaccagtgca
      841 tttttcgtgt cgtcatcgtc cttctgcaaa tctaagtcat cttgtctgca ccaaatggga
      901 taaccgtcgg gtctccgccc cgactctaag aaggaggacg tggcctccag ctcaccactg
      961 ttcttcagca aggcctgtgt aactgttgat agatccaagt tgaacttctc cattagctgc
     1021 cggatcacct taatggcagt tcccacttct tgcgtagaaa cttttggttc ttcttcctcc
     1081 tcgtctggct gtgtttctga gtcttcctcg ggtgtgggtg gatcaccatc acacatcgtt
     1141 atatgtattt caaagtcagg gctctcatcc accacggctt ctccgaactc cggagcggct
     1201 tcctcaaaca gcttgttgtc tgcctctcca ttctccttga tctctccttt cacacactcc
     1261 tcctcaggca agtctggcgc tctcttgttc tgtggctctc cgctatccgc ggcctcgggg
     1321 tcctgctccg ccttccgttt gagcttctgg gaggacgggc tgaccggggc gttcccgagc
     1381 aggtacttgt gctcctgacc ccgtaggtgc ttgaggtagc ggtccttgag cgactgccag
     1441 gagtgctgcg tgagcgagct cttctccatc gctttccaca aggcattgcc tgtgaccgag
     1501 ctgggcgaac gggcgttctc cttcacgtag gtcaggatgg ccacatcttc cgcgtcggtg
     1561 taggcgatgc gcccggtcag gggctgcggc tccggttccg tggagccctc ggtggaagcc
     1621 ccgggcttcg gatcggacgc ctgctccgtc aggcccagcc gataggcctc caggtccagc
     1681 ttctcgttgc gatccacgca gtctaggatg tactgcgtgg agatgaagtc gcccgaagcc
     1741 tcggccagcg cctccccggg ctgggcgaga agcacggctc cgggttcctg cacccgacac
     1801 acggtgccgc cgccgtgcag gatgagcgtc gacagccggc gcttggccga gctgggccgc
     1861 acgtaaaacg acatggcgct gccgtcttct ctcacgaaca gagtggagga gtgagtgggc
     1921 ccattggggt ctttacccag atccatcgcc tccgcggatc ctctctttct taacaaatct
     1981 tcttcagaaa tcaacttttg ttccatggtg gctgatccga gctcggtacc aagcttgggt
     2041 ctccctatag tgagtcgtat taatttcgat aagccagtaa gcagtgggtt ctctagttag
     2101 ccagagagct ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa
     2161 tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact
     2221 cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg
     2281 cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat
     2341 gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca
     2401 tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc
     2461 caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg
     2521 cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca
     2581 gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg ggctatgaac
     2641 taatgacccc gtaattgatt actattaata actagtcaat aatcaatgtc aacgcgtata
     2701 tctggcccgt acatcgcgaa gcagcgcaaa acgcctaacc ctaagcagat tcttcatgca
     2761 attgtcggtc aagccttgcc ttgttgtagc ttaaattttg ctcgcgcact actcagcgac
     2821 ctccaacaca caagcaggga gcagatactg gcttaactat gcggcatcag agcagattgt
     2881 actgagagtg caccataggg gatcgggaga tccgatgcat ggggtcgtgc gctcctttcg
     2941 gtcgggcgct gcgggtcgtg gggcgggcgt caggcaccgg gcttgcgggt catgcaccag
     3001 gtgcgcggtc cttcgggcac ctcgacgtcg gcggtgacgg tgaagccgag ccgctcgtag
     3061 aaggggaggt tgcggggcgc ggaggtctcc aggaaggcgg gcaccccggc gcgctcggcc
     3121 gcctccactc cggggagcac gacggcgctg cccagaccct tgccctggtg gtcgggcgag
     3181 acgccgacgg tggccaggaa ccacgcgggc tccttgggcc ggtgcggcgc caggaggcct
     3241 tccatctgtt gctgcgcggc cagccgggaa ccgctcaact cggccatgcg cgggccgatc
     3301 tcggcgaaca ccgcccccgc ttcgacgctc tccggcgtgg tccagaccgc caccgcggcg
     3361 ccgtcgtccg cgacccacac cttgccgatg tcgagcccga cgcgcgtgag gaagagttct
     3421 tgcagctcgg tgacccgctc gatgtggcgg tccggatcga cggtgtggcg cgtggcgggg
     3481 tagtcggcga acgcggcggc gagggtgcgt acggccctgg ggacgtcgtc gcgggtggcg
     3541 aggcgcaccg tgggcttgta ctcggtcatg gtaagctgat ccggccggcg cctagagaag
     3601 gagtgagggc tggataaagg gaggatcgag gcggggtcga acgaggaggt tcaaggggga
     3661 gagacggggc ggatggagga agaggaggcg gaggcttagg gtgtacaaag ggcttgaccc
     3721 agggaggggg gtcaaaagcc aaggcttccc aggtcacgat gtaggggacc tggtctgggt
     3781 gtccatgcgg gccaggtgaa aagaccttga tcttaacctg ggtgatgagg tctcggttaa
     3841 aggtgccgtc tcgcggccat ccgacgttaa aggttggcca ttctgcagag cagaaggtaa
     3901 cccaacgtct cttcttgaca tctaccgact ggttgtgagc gatccgctcg acatctttcc
     3961 agtgacctaa ggtcaaactt aagggagtgg taacagtctg gccctaattt tcagacaaat
     4021 acagaaacac agtcagacag agacaacaca gaacgatgct gcagcgctgc agcagacaag
     4081 acgcgcggct tcggttccaa accgaaagca aaaattcaga cggaggcggg aactgtttta
     4141 ggttctcgtc tcctaccaga accacatatc ctgacggggt cggattccac atcgactccc
     4201 ttcctcaggt cgggccacaa aaacggcccc caaagtccct gggacgtctc ccagggttgc
     4261 ggccgggtgt tcagaactcg tcagttccac cacgggtccg ccagatacag agctagttag
     4321 ctaactagta ccgacgcagg cgcataaaat cagtcataga cactagacaa tcggacagac
     4381 acagataagt tgctggccag cttgcctccc ggtggtgggt cggtggtccc tgggcagggg
     4441 tctcccgatc ccggacgagc ccccaaatga aagacccccg ctgacgggta gtcaatcact
     4501 cagaggagac cctcccaagg aacagcgaga ccacaagtcg gatgcaactg caagagggtt
     4561 tattggatac acgggtaccc gggcgactca gtcaatcgga ggactggcgc cccgagtgag
     4621 gggttgtggg ctcttttatt gagctcgggg agcagaagcg cgcgaacaga agcgagaagc
     4681 gaactgattg gttagttcaa ataaggcaca gggtcatttc aggtccttgg ggcaccctgg
     4741 aaacatctga tggttctcta gaaactgctg agggctggac cgcatctggg gaccatctgt
     4801 tcttggccct gagccggggc aggaactgct taccacagat atcctgtttg gcccatattc
     4861 agctgttcca tctgttcttg gccctgagcc ggggcaggaa ctgcttacca cagatatcct
     4921 gtttggccca tattcaggct gcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     4981 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     5041 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     5101 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     5161 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     5221 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     5281 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     5341 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     5401 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     5461 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     5521 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     5581 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     5641 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     5701 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     5761 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     5821 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     5881 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     5941 accaagttta ctcatatata ctttagattg atttgcggcc gcaaaaggat ctaggtgaag
     6001 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     6061 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     6121 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     6181 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     6241 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     6301 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     6361 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     6421 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     6481 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     6541 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     6601 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     6661 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     6721 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     6781 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     6841 tcagtgagcg aggaagcgga ag
//
