LOCUS       sequence-137337-       10496 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the Dscaml1 protein, 
            C-terminally fused to the Fc region of human IgG1 + 6X histidine 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10496)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 10496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Dscaml1-Fc-His
FEATURES             Location/Qualifiers
     source          1..10496
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             7076..7093
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    7119..7240
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7156..7175)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7210..7229
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7278..7295)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7449..8037)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7529..7548)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8208..8999,9000..9068))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8831..8850
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9069..9173)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9241..9259
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9297..9319)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9321..9341)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9326..9656
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9507..9642
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9569..9588
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     9793..9812
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(9879..10334)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10016..10037)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10228..10247
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10460..10482
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10474..10491
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10475..10491
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tgtggctggt
     1621 aactttcctc ctgctcctgg actctttaca caaagcccgc cccgaggatg tcggcaccag
     1681 cctctacttt gtaaatgact ccctgcagca cgtgaccttc tccagttctg tgggtgtggt
     1741 ggtgccctgc ccggcagctg gatcccccag tgcggctctc cgatggtacc tggccacggg
     1801 ggacgacatc tacgatgtgc cacacatccg gcacgtgcat gccaacggga ctctgcagct
     1861 cttccctttc tccccctccg ccttcaatag ctttatccac gacaatgact atttctgcac
     1921 tgcagagaac gcagccggca agatccggag ccctaacatt cgcatcaaag cagttttcag
     1981 ggaaccctac accgtccggg tggaggatca aaggtcaatg cgtggcaacg tggctgtctt
     2041 taagtgcctc atcccctctt cagtgcagga atacgttagc gtcgtgtctt gggagaaaga
     2101 cacggtctcc atcactccag aaaacaggtt tttcatcaca tcacatggtg gactgtacat
     2161 ctcggacgtg cagaaggaag atgctctctc cacctaccgc tgcatcaccc agcacaaata
     2221 cagtggggag actcgtcaga gcaacggagc tcggctctct gtgacagacc ctgcagagtc
     2281 catccctacc atcctggatg gcttccactc ccaggaagtg tggactggcc actcggtaga
     2341 gctgccctgt gctgcttccg gctaccccat ccctgccatc cgctggctca aagatggcag
     2401 acccctccct gctgacagcc gctgggctaa gcgcattaca gggctgacca tcagtgacct
     2461 gcgcactgag gacagcggca cctacatctg tgaggtcacc aacacctttg ggtcagcaga
     2521 ggccaacggc atcctcacag tcattgaccc tcttcatgtg accctgacac caaagaagct
     2581 gaagacgggc atcgggagca cggtcatcct atcctgcgcc ctcacaggct ccccagagtt
     2641 caccatccgt tggtaccgca acacagaact ggtgctgccg ggtgaggcca tctccatccg
     2701 tgggctcagc aatgagactc tgctcatctc ctcggcacag aagagccact cgggagccta
     2761 ccagtgcttc gccacccgca aggcccagac agcccaggac tttgccatca ttgtgctgga
     2821 agacggcacg ccccgcatcg tctcatcctt cagcgagaag gtggtcaacc ctggggagca
     2881 gttctcactg atgtgtgccg ccaagggcgc cccgcccccc acggtcacct gggccctgga
     2941 cgacgagccc gttgtgcggg acggaagcca ccgcaccaac cagtacacca tgtccgacgg
     3001 caccaccatc agccacatga acgtcacggg tccccagatc cgggatgggg gcgtgtaccg
     3061 gtgcacagcg cggaactcgg tgggcagtgc tgaatatcag gcgcgaataa acgtaagagg
     3121 accccccagc atccgggcaa tgaggaacat cacggcagtc gccgggcggg acacactcat
     3181 caactgcagg gtcattggct acccctacta ctccatcaag tggtacaagg atgccctgct
     3241 gctgcccgat aaccaccgcc aggtggtgtt tgagaatggg actctcaagc tgacggatgt
     3301 gcagaagggc atggatgagg gggaatacct gtgcagtgtt ctcatccagc cacagctttc
     3361 catcagccag agtgtccacg tggccgtcaa agtgccccct ctgatccagc ccttcgagtt
     3421 cccccctgcc tccatcggcc agctgctcta cattccctgt gtggtgtcct cgggggacat
     3481 gccaatccgc atcacctgga ggaaggacgg acaggtgatc atctcaggct caggcgtgac
     3541 catcgagagc aaggagttca tgagctccct gcagatctcc agcgtctccc tcaagcacaa
     3601 cggcaactac acttgcatcg ccagcaacgc agcggccact gtgagcaggg agcgccagct
     3661 catcgtgcgt gtgccccctc gatttgtggt gcagcccaat aaccaagatg gcatctacgg
     3721 caaagctggt gtgctcaact gctcggtaga tggctaccca ccacccaagg tgatgtggaa
     3781 gcatgccaaa gggagtggga acccccagca atatcacccg gtgcccctca ctggccgcat
     3841 ccagatccta cccaacagct ccctgctgat acgccacgtc ctggaagagg acatcggcta
     3901 ctacctctgc caggccagca acggcgtggg caccgacatc agcaaggcca tgttcctcac
     3961 cgtgaaaatc ccagcaatga tcacatctca ccccaacacc acgatcgcca tcaagggcca
     4021 ccccaaggag ctgaactgca ctgcgcgtgg cgagcggccc atcataatcc gctgggagaa
     4081 gggggacaca gtcattgatc ccgaccgtgt catgcgctat gccattgcta ccaaggacaa
     4141 tggcgatgaa gtggtctcca cgcttaagct caagccagct gaccgtgggg actccgtgtt
     4201 cttcagctgc catgctatca actcatatgg agaggaccga ggtttgatcc agctcactgt
     4261 gcaagagccc cctgaccccc cagagctaga gatccgggag gtgaaggccc ggagcatgaa
     4321 cctgcgctgg actcagagat ttgatggaaa cagcatcatc accggctttg atattgaata
     4381 caagaacaaa tcagattcct gggattttaa gcagtccaca cgcaacatct ccccaactat
     4441 caaccaggcg aacatcgtgg acttacaccc tgcatcggtg tacagcatcc gcatgtactc
     4501 cttcaacaag atcgggcgca gcgagccaag caaggagctt accatcagta cggaagaggc
     4561 tgctcctgat gggcctccca tggatgtcac cttgcaacca gtgacttcac agagcatcca
     4621 ggtgacctgg aaggcaccaa agaaggagct gcagaatgga gtcatcaggg gataccagat
     4681 tggctaccgg gagaacagcc ctggcagtaa tgggcagtac agcattgtgg aaatgaaggc
     4741 gacgggggac agtgaggtct acaccctgga taacctcaag aagttcgctc agtacggcgt
     4801 ggtggtccaa gccttcaacc gggcaggcac ggggccctca tctagtgaga tcaatgccac
     4861 caccttggag gatgtgccca gccagcctcc agagaatgtc cgagctctgt ccatcacttc
     4921 tgatgtggct gtcatctcct ggtcggagcc cccacgaagt accctcaatg gtgtcctcaa
     4981 aggctaccgg gtcatcttct ggtcactcta cgttgatgga gagtggggcg agatgcagaa
     5041 tgtcaccacc acaagggagc gagtggagct tcgcggtatg gagaagttca ccaactacag
     5101 cgtccaggtg ctcgcctaca cgcaggctgg ggatggtgtg cgcagcagcg tgctctacat
     5161 ccagaccaag gaagatgttc caggcccccc tgctggcatc aaagctgtcc cttcatcagc
     5221 tagcagtgtg gtcgtgtcct ggctgccccc aaccaagccc aatggggtga tccgcaaata
     5281 caccatcttc tgttccagcc ctgggtccgg tcagccggct cccagcgagt acgagaccag
     5341 tcctgaacag ctcttctacc ggatcgccca cctgaaccgg ggacagcagt acttgctatg
     5401 ggtggctgcg gtcacctctg caggcagggg gaacagcagc gagaaggtca ccattgaacc
     5461 tgctggcaag gccccagcaa agatcatctc atttggaggc actgtaacaa caccctggat
     5521 gaaagatgtc cggctgcctt gcaattcagt aggagatcca gccccggctg tgaagtggac
     5581 caaggacagt gaagactcag caatccccgt gtccttggat ggacaccggc ttatccacac
     5641 aaacggcaca ctgctgcttc gtgctgtgaa ggctgaagac tccggctatt atacctgcac
     5701 agccaccaac actggtggct ttgataccat catcgtcaac cttctggtac aagttccccc
     5761 ggaccagccc cgcctcactg tctccaaaac ctctgcctct tctatcaccc tgacctggat
     5821 tccaggagac aatgggggca gctccatcag aggcttcgtg ttgcaatact ctgtggacaa
     5881 cagcgaggag tggaaggatg tgttcatcag ctctagcgag cgctccttca agctagacag
     5941 cctcaagtgt ggcacgtggt acaaggtgaa gctggcggcc aagaacagtg tgggctctgg
     6001 gcgcatcagc gagatcatcg aggccaagac ccatgggcgg gagccttcct tcagcaaaga
     6061 ccaacacctc ttcacccaca tcaactccac acatgctcgg cttaacctgc agggctggaa
     6121 caatggaggt tgtcccatca cagccattgt cctggagtac cggcccaagg ggacctgggc
     6181 ctggcagggc gtccgagcca acagctccac agaggtgttt ctgacagaac ttcgagaggc
     6241 tacgtggtat gagctacgca tgagggcttg caacagcgcc ggctgtggca acgagaccgc
     6301 ccagtttgcc accctggact acgacggcag caccatccca cccatcaaat ctgctcaagg
     6361 agaaggcgat gacgtgaaga agactagtga caaaactcac acatgcccac cgtgcccagc
     6421 acctgaactc ctggggggac cgtcagtctt cctcttcccc ccaaaaccca aggacaccct
     6481 catgatctcc cggacccctg aggtcacatg cgtggtggtg gacgtgagcc acgaagaccc
     6541 tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg cataatgcca agacaaagcc
     6601 gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc gtcctcaccg tcctgcacca
     6661 ggactggctg aatggcaagg agtacaagtg caaggtctcc aacaaagccc tcccagcccc
     6721 catcgagaaa accatctcca aagccaaagg gcagccccga gaaccacagg tgtataccct
     6781 gcccccatcc cgggatgagc tgaccaagaa ccaggtcagc ctgacctgcc tggtcaaagg
     6841 cttctatccc agcgacatcg ccgtggagtg ggagagcaat gggcagccgg agaacaacta
     6901 caagaccacg cctcccgtgc tggactccga cggctccttc ttcctctaca gcaagctcac
     6961 cgtggacaag agcaggtggc agcaggggaa cgtcttctca tgctccgtga tgcatgaggc
     7021 tctgcacaac cactacacgc agaagagcct ctccctgtct ccgggtaaag gcggccatca
     7081 tcaccatcac cattaatcta gagatcttat taaagcagaa cttgtttatt gcagcttata
     7141 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     7201 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg tcgactctag
     7261 ctagactctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7321 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     7381 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7441 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     7501 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     7561 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7621 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     7681 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7741 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     7801 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7861 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     7921 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     7981 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     8041 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     8101 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     8161 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     8221 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     8281 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     8341 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     8401 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     8461 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     8521 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     8581 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     8641 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     8701 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     8761 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     8821 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     8881 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     8941 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     9001 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     9061 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     9121 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     9181 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     9241 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     9301 tgacacatgc agctcccgga ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     9361 cccaggcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     9421 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     9481 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     9541 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     9601 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     9661 tcccgggagc ttgtatatcc attttcgtcc cggagacggt cacagcttgt ctgtaagcgg
     9721 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     9781 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     9841 taccgcacag atgcgtaagg agaaaatacc gcatcaggaa attgtaagcg ttaatatttt
     9901 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     9961 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
    10021 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
    10081 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
    10141 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
    10201 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
    10261 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
    10321 gctacagggc gcgtccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
    10381 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
    10441 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaatt
//
