LOCUS       sequence-137338-         569 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Streptococcus pyogenes nCas9 (H840A) nickase carrying two 
            C-terminal SV40 NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Richardson CD, Ray GJ, DeWitt MA, Curie GL, Corn JE
  TITLE     Enhancing homology-directed genome editing by catalytically active 
            and inactive CRISPR-Cas9 using asymmetric donor DNA.
  JOURNAL   Nat Biotechnol. 2016 Jan 20. doi: 10.1038/nbt.3481.
  PUBMED    26789497
REFERENCE   2  (bases 1 to 569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 20. doi: 10.1038/nbt.3481."
COMMENT     Sequence Label: pCR1055
FEATURES             Location/Qualifiers
     source          1..569
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 attatcaaac ttacgttgag tgattaactt ggcgtttaga agttgtctcc aatagttttt
       61 catctttttg actacttctt cacttggaac gttatccgat ttaccacgat ttttatcaga
      121 acgcgttaag accttattgt ctattgaatc gtctttaagg aaactttgtg gaacaatggc
      181 atcgacatca taatcactta aacgattaat atctaattct tggtccacat acatgtctct
      241 tccattttgg agataataga gatagagctt ttcattttgc aattgagtat tttcaacagg
      301 atgctcttta agaatctgac ttcctaattc tttgatacct tcttcgattc gtttcatacg
      361 ctctcgcgaa tttttctggc ccttttgagt tgtctgattt tcacgtgcca tttcaataac
      421 gatattttct ggcttatgcc gccccattac tttgaccaat tcatcaacaa cttttacagt
      481 ctgtaaaata ccttttttaa tagcagggct accagctaaa tttgcaatat gttcatgtaa
      541 actatcgcct tgtccagaca cttgtgctt
//
