LOCUS       sequence-137351-         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of shRNA against CRTC2 (3rd generation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Jeanneteau FD, Lambert WM, Ismaili N, Bath KG, Lee FS, Garabedian 
            MJ, Chao MV
  TITLE     BDNF and glucocorticoids regulate corticotrophin-releasing hormone 
            (CRH) homeostasis in the hypothalamus.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Jan 24;109(4):1305-10. doi: 
            10.1073/pnas.1114122109. Epub 2012 Jan 9.
  PUBMED    22232675
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1114122109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-01-24- 24"; volume: "109"; issue: "4"; pages: "1305-10"
COMMENT     Sequence Label: pLL3.7 shRNA against CRTC2
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      9..18
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(57..260)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(86..106)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        261..564
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    680..713
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(755..771)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(756..772)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 cttgctcacc atggtggcga ccggtagcgc tagcggatct gacggttcac taaaccagct
       61 ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag
      121 ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg
      181 tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg
      241 tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat gtactgccaa
      301 gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca
      361 ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca
      421 gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg cgttactatg
      481 ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg
      541 ccatttaccg taagttatgt aacgcggaac tccatatatg ggctatgaac taatgacccc
      601 gtaattgatt actattaata actaatgcat ggcggtaata cggttatcca cgcggccgct
      661 taagcttgga acccttaata taacttcgta taatgtatgc tatacgaagt tattaggtcc
      721 ctcgacctgc tggaatctcg tgaagcgagc ttatcgatac cgtcgacctc gagaa
//
