LOCUS       sequence-137353-        8915 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the Contactin 5, isoform 1 
            protein (truncated immediately upstream of propeptide cleavage and 
            GPI-attachement site), C-terminally fused to the Fc region of human 
            IgG1 + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8915)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 8915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Cntn5.1-Fc-His
FEATURES             Location/Qualifiers
     source          1..8915
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      1604..1613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5495..5512
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    5538..5659
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5575..5594)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5629..5648
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5697..5714)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5868..6456)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5948..5967)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6627..7418,7419..7487))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7250..7269
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7488..7592)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7660..7678
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7716..7738)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7740..7760)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7745..8075
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7926..8061
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7988..8007
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8212..8231
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(8298..8753)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8435..8456)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8647..8666
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8879..8901
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8893..8910
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8894..8910
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tggcttcctg
     1621 ttggagactc attctgttcc tgtctgtcac caggtggctt tcagattact ctgaagctct
     1681 ctctggcctt tctacttcat atgctgcttt gctcagaatt aagaagagtt caacctcacc
     1741 ctttggttcc aagtccagac cacggttcag cagtccttca ttaggtacca tcagtgtttc
     1801 tccaccaagc tggcgagggg cagctcagca gtaccactcc ccaggcaacc tttatcactc
     1861 ctcagatgcc ttcagacaag atgaaagtgt ggattatggg ccagtatttg tgcaagaacc
     1921 tgatgatgtc atttttccaa ctgattctga tgaaaagaag gtagctctaa attgtgaagt
     1981 tcgtggcaac ccatctccta gttacagatg gcttcggaat ggaactgaaa tagctctgga
     2041 gagtgactat cgctacagtc tgatagacgg caccttcatc atcagcaatc caagtgaact
     2101 cagggattct ggcctgtacc agtgcttagc caccaactct tttggaagta ttcttagtcg
     2161 agaagccact cttcagtttg cctatctggg aaattttagt ggccggacaa gaagtgcagt
     2221 ctcagtgcgg gaaggccagg gggttgttct gatgtgctct ccaccaccac attctccaga
     2281 gatcatctat agctgggtct tcaatgagtt cccttccttt gtggcagagg acagccggcg
     2341 gttcatatcc caagagacag gcaaccttta tatttctaaa gtgcaaacat ccgatgttgg
     2401 cagctacatt tgtctggtga aaaatgctgt gacaaatgct cgcgtgctca gtcctccaac
     2461 cccactcacg ctgcgaaatg atggtgtgat gggagaatat gagccaaaaa ttgaggtcca
     2521 ttttcctttc actgtcacag ctgctaaggg aacaactgtg aagatggaat gctttgcact
     2581 tggcaacccg gttccaacaa ttacttggat gaaggttaac ggttacattc ctagtaagtc
     2641 cagactgcgg aaatcccagg cagtgctgga gattccaaac ctgcagctgg atgatgccgg
     2701 aatatacgaa tgcacagctg aaaactcccg tggaaaaaac tcctttcgtg gacagctaca
     2761 aatattcaca tacccacact gggtacagaa actgaatgat acccagctgg acagtgggag
     2821 tcctctgcag tgggaatgca aggctactgg aaagcccaga cccacatacc gctggctgaa
     2881 gaatggagca cccctcttgc cgcagagcag agtggacaca gtgaatggaa tattggcgat
     2941 ccagagtgtg aatcaatcag atgctgggat gtatcagtgt ttggctgaga ataaatatgg
     3001 agctatttat gcgagtgctg agctgaagat tctagcttcg ccgccctcct tcgaactgaa
     3061 tcaagtgaag aaatccataa ttgttaccaa agaccgggga gtgctcatag agtgcgaacc
     3121 ccagggatct ccaaagccag ccatctcctg gaggaaggga gacaaggcag tgagggcgaa
     3181 taaaagaata gctattcttc cagacggaag cctgaggatc ctaaacgctt ctaaagcaga
     3241 tgaggggaag tacatttgcc aaggggtgaa catcttcggt tctgcagaga ttatagcttc
     3301 cctgtctgtc aaagaaccca caaggataga gcttactcct aaaagaacag agttaactgt
     3361 tggagagagc attgtcctca actgtaaagc tatccacgac gccagtttgg acgtcacttt
     3421 ctactggaca ttaaaaggac aacctattga ttttgagaaa gaaggcggac attttgaaaa
     3481 catcagggct caagcatcct ctgcagattt aatgatcagg aacatccttc tgatgcatgc
     3541 tgggagatat ggctgcaggg tacagaccac agcagacagt gtgtcagatg aggcagaact
     3601 tcttgttagg ggtcccccag ggccacctgg agtggtcata gtagaggaga ttacagaaag
     3661 cacagccact ctctcatgga gtccagcaac tgacaaccat agccccatct cttcttacaa
     3721 ccttcaggct cgcagcccct tctccctggg ctggcaaaca gtgaagacag tcccagaagt
     3781 catcacagga gacatggagt cagcaatggc tgtggaccta aacccctggg tagaatatga
     3841 attccgagtg gtggccacca atccaattgg gacaggagat ccaagcattc catctcgaat
     3901 gatccgcaca aatgaggcag ttccaaagac ggcacccagc aacgtgagtg gaagaagtgg
     3961 aagaaggcat gagttggtca ttgcctggga gccagtatca gaagagtttc agaatgggga
     4021 aggcttcggc tacatagtgg cattcagacc taatggaaca cgtggctgga aggagaaaat
     4081 ggttacgtct tcggaggcgt ccaagttcat ttaccgagat gaaagtgtcc ctcctctcac
     4141 accctttgag gtcaaagttg gtgtttataa taataaaggt gatgggcctt tcagccagat
     4201 cgtagtcatc tgttcagctg agggagaacc cactgctgct cccactgacg tcacagcaac
     4261 cagtgtgtca gtgtcagaga tttttgttgt atggaaacat gttaaggaga gtcttggaag
     4321 accacagggg tttgagatta gttactggaa agataccgag ccagaagact ccgtagaaac
     4381 agtccgaacc agagggaatg agtcattcgt catgttgacg ggattggaag gcaacacact
     4441 ctatcacctc acagtcaggg cttacaatgg agccggctat gggccaccca gcagggaagc
     4501 aagcaccacc acaaagaggc acccccctag ggagccacct ggcaacctcc ggtgggagca
     4561 gcagggctcc caagtttccc taggctggga gccagtcaga cccctagcca atgagtctga
     4621 agtcatgggt tacaaggttt tttataggca agagggtcac agcgaaggtc aagttattga
     4681 gacacagaaa cctcaagctg tagtgcctct acctgaggct ggagtctaca ttattgaagt
     4741 tcgagcgtac agtgagggag gagatggaac agctagctcc caaatcaggg ttccttcata
     4801 tactagtgac aaaactcaca catgcccacc gtgcccagca cctgaactcc tggggggacc
     4861 gtcagtcttc ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga
     4921 ggtcacatgc gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta
     4981 cgtggacggc gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag
     5041 cacgtaccgt gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga
     5101 gtacaagtgc aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa
     5161 agccaaaggg cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct
     5221 gaccaagaac caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc
     5281 cgtggagtgg gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct
     5341 ggactccgac ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca
     5401 gcaggggaac gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca
     5461 gaagagcctc tccctgtctc cgggtaaagg cggccatcat caccatcacc attaatctag
     5521 agatcttatt aaagcagaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     5581 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     5641 aactcatcaa tgtatcttat catgtctggt cgactctagc tagactcttc cgcttcctcg
     5701 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     5761 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     5821 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     5881 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     5941 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     6001 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     6061 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     6121 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     6181 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     6241 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     6301 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     6361 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     6421 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     6481 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     6541 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     6601 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     6661 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     6721 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     6781 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     6841 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     6901 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     6961 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     7021 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     7081 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     7141 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     7201 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     7261 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     7321 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     7381 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     7441 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     7501 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     7561 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     7621 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     7681 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     7741 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccaggcaggc agaagtatgc
     7801 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     7861 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     7921 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     7981 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     8041 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
     8101 ttttcgtccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     8161 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     8221 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     8281 gaaaataccg catcaggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     8341 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     8401 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     8461 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     8521 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     8581 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     8641 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     8701 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtccattcg
     8761 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     8821 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     8881 cagtcacgac gttgtaaaac gacggccagt gaatt
//
