LOCUS       sequence-137359-        8705 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the Contactin 3 protein 
            (truncated immediately upstream of propeptide cleavage and 
            GPI-attachement site), C-terminally fused to the Fc region of human 
            IgG1 + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8705)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 8705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Cntn3-Fc-His
FEATURES             Location/Qualifiers
     source          1..8705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             5285..5302
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    5328..5449
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5365..5384)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5419..5438
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5487..5504)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5658..6246)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5738..5757)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6417..7208,7209..7277))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7040..7059
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7278..7382)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7450..7468
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7506..7528)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7530..7550)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7535..7865
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7716..7851
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7778..7797
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8002..8021
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(8088..8543)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8225..8246)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8437..8456
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8669..8691
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8683..8700
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8684..8700
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tgatgttgtc
     1621 atggaaacag ttgatcctgc tttcattcat tggctgcctg gcaggtgaac ttcttttaca
     1681 aggccctgtg tttatcaaag agcccagcaa cagtattttc cctgttgatt cagaggacaa
     1741 aaaaataaca ttaaattgtg aagcaagagg caacccttca cctcattaca ggtggcagct
     1801 gaatggcagt gatattgata caagtctgga ccatcgttac aaactgaatg gaggaaatct
     1861 catagttatt aaccctaaca gaaattggga tacaggaagt taccagtgtt ttgcaacgaa
     1921 ttctcttgga acaatagtga gcagagaagc caaactccag tttgcctatc ttgaaaattt
     1981 taagaccagg atgagaagca ctgtgtcggt ccgtgaaggc cagggagtgg tgcttctctg
     2041 tggaccccca cctcactcag gagaactgtc atatgcttgg gtcttcaatg agtatccatc
     2101 atttgttgaa gaagacagtc gacgatttgt ctctcaagag acaggacacc tctacatagc
     2161 taaggtggaa ccatctgatg tgggaaatta cacatgtgtg gtgaccagca cggtgacaaa
     2221 taccagagta ttgggctccc caaccccttt ggtgctacgc tctgatggtg tcatgggtga
     2281 atatgaaccc aaaatagaag tccagtttcc agagactctt ccagcagcta aaggttcaac
     2341 tgtgaggttg gaatgcttcg ccctgggaaa ccctgttcct cagattaatt ggagaagaag
     2401 tgatgggatg ccgtttccca acaaaattaa gttgaggaag ttcaatggta tgctggaaat
     2461 tcaaaacttc caacaggaag atacaggctc ctatgagtgt attgctgaaa attcacgagg
     2521 aaaaaatgtg gcgagagggc ggctcactta ctatgcaaaa ccttattggc ttcaactctt
     2581 aagagatgtg gaaattgctg tggaggacag tctttattgg gaatgccggg caagtgggaa
     2641 gcctaaaccc tcttatcgat ggctgaaaaa tggagatgcc ttagtactag aggagagaat
     2701 acagatagaa aatggtgccc ttacaataac caacctcaat gtgactgact ctggaatgtt
     2761 ccaatgtata gcagaaaaca agcatggtct catctactcc agtgctgagc tcaaggtagt
     2821 agcttctgct ccagattttt caagaaaccc tatgaaaaaa atggtccagg tgcaggtggg
     2881 cagcctggtg atcctggatt gtaaacccag agcctctcca agggcacttt ctttctggaa
     2941 gaagggagac atgatggtgc gggagcaggc aagggtttct tttttaaatg atggaggact
     3001 caaaatcatg aatgtgacta aggcagacgc tggaacctac acctgcacag cagaaaacca
     3061 gtttgggaaa gcaaatggca caacccactt ggttgtgaca gaacctacga gaataatttt
     3121 ggcaccttca aacatggacg ttgctgttgg tgagagcgtc attttaccct gccaggttca
     3181 acatgatccc ctattagaca taatgtttgc ctggtatttc aatggagccc ttacagactt
     3241 taagaaagat ggatctcact ttgagaaagt tggtgggagt tcatctggtg atttaatgat
     3301 cagaaacatt caattgaaac acagtgggaa gtacgtatgt atggtgcaga caggggtgga
     3361 cagtgtttcc tctgcagctg agctcatcgt gagaggttca cctgggccac cggaaaatgt
     3421 gaaggtagat gaaattacag acacaacagc ccagctctct tggacggaag gcacggacag
     3481 tcatagccca gttatatcgt atgcagtcca agctaggacg cctttctctg tgggctggca
     3541 aagtgtcaga acagtacctg aggtcattga tggcaagaca catacagcta ctgtcgtgga
     3601 gttaaaccca tgggtggaat atgaatttcg cattgtagcc agtaacaaaa tcggaggtgg
     3661 agaacccagt ttaccctcag aaaaagtaag gactgaagag gcagctccag aaatcgcacc
     3721 ttctgaggtc agcggaggag gtggaagccg atctgaacta gtgataacct gggatccagt
     3781 ccctgaagaa ctacagaatg gaggaggttt tgggtatgtg gttgctttcc gcccacttgg
     3841 ggttaccacc tggatccaga cagtggtgac atccccagat aacccaagat acgtctttag
     3901 gaatgaaagc attgtgccct tttcgccata tgaagttaaa gtgggagttt acaataacaa
     3961 aggtgaagga ccctttagcc cagtgacaac tgtgttctct gcagaggaag agcctacagt
     4021 tgccccatct cacatctctg cccatagcct gtcttcctca gaaattgaag tttcttggaa
     4081 tacaattccc tggaagttga gcaatggaca tttactgggc tatgaggtgc ggtactggaa
     4141 caatggagga gaagaagaat catcccgcaa agtgaaagtg gcaggaaatc agacatcagc
     4201 tgtgctccga ggcctgaaga gcaacctggc ctattacaca gctgtccggg cttacaacag
     4261 cgcaggcgct ggtcccttca gcgccacagt taatgcgacc accaagaaaa cccctcccag
     4321 tcagccacca ggaaatgttg tttggaatgc tacagacact aaagtgctac ttaattggga
     4381 acaagtgaaa gccatggaga atgaatcaga agtaacaggc tacaaggttt tctataggac
     4441 tagcagtcag aataatgtgc acgtgctgaa cacaaataaa acttcagctg aactgttgct
     4501 gcctatcaaa gaagactaca ttattgaggt caaggccacc acagatggag gggatggcac
     4561 cagtagtgag cagatcagga ttccaagaat aactagtgac aaaactcaca catgcccacc
     4621 gtgcccagca cctgaactcc tggggggacc gtcagtcttc ctcttccccc caaaacccaa
     4681 ggacaccctc atgatctccc ggacccctga ggtcacatgc gtggtggtgg acgtgagcca
     4741 cgaagaccct gaggtcaagt tcaactggta cgtggacggc gtggaggtgc ataatgccaa
     4801 gacaaagccg cgggaggagc agtacaacag cacgtaccgt gtggtcagcg tcctcaccgt
     4861 cctgcaccag gactggctga atggcaagga gtacaagtgc aaggtctcca acaaagccct
     4921 cccagccccc atcgagaaaa ccatctccaa agccaaaggg cagccccgag aaccacaggt
     4981 gtataccctg cccccatccc gggatgagct gaccaagaac caggtcagcc tgacctgcct
     5041 ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg gagagcaatg ggcagccgga
     5101 gaacaactac aagaccacgc ctcccgtgct ggactccgac ggctccttct tcctctacag
     5161 caagctcacc gtggacaaga gcaggtggca gcaggggaac gtcttctcat gctccgtgat
     5221 gcatgaggct ctgcacaacc actacacgca gaagagcctc tccctgtctc cgggtaaagg
     5281 cggccatcat caccatcacc attaatctag agatcttatt aaagcagaac ttgtttattg
     5341 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     5401 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggt
     5461 cgactctagc tagactcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     5521 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     5581 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     5641 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     5701 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     5761 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     5821 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     5881 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     5941 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     6001 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     6061 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     6121 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     6181 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     6241 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     6301 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     6361 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     6421 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     6481 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     6541 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     6601 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     6661 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     6721 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     6781 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     6841 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     6901 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     6961 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     7021 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     7081 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     7141 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     7201 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     7261 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     7321 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     7381 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     7441 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
     7501 gaaaacctct gacacatgca gctcccggag gaatgtgtgt cagttagggt gtggaaagtc
     7561 cccaggctcc ccaggcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     7621 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     7681 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     7741 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     7801 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     7861 gcaaaaagct cccgggagct tgtatatcca ttttcgtccc ggagacggtc acagcttgtc
     7921 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     7981 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     8041 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggaaa ttgtaagcgt
     8101 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     8161 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     8221 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     8281 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     8341 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     8401 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     8461 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     8521 taatgcgccg ctacagggcg cgtccattcg ccattcaggc tgcgcaactg ttgggaaggg
     8581 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
     8641 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
     8701 gaatt
//
