LOCUS       sequence-137371-        1079 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All-in-one doxycycline inducible lentiviral vector for expression of
            one gene in combination with turbo GFP using the P2A self-cleaving 
            peptide..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Barger CJ, Branick C, Chee L, Karpf AR
  TITLE     Pan-Cancer Analyses Reveal Genomic Features of FOXM1 Overexpression 
            in Cancer.
  JOURNAL   Cancers (Basel). 2019 Feb 21;11(2). pii: cancers11020251. doi: 
            10.3390/cancers11020251.
  PUBMED    30795624
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancers 
            (Basel)."; date: "2019-02-21"; volume: "11(2). pii: cancers11020251.
            doi"; pages: " 10.3390/cancers11020251"
COMMENT     Sequence Label: pCW57-GFP-2A-MCS
FEATURES             Location/Qualifiers
     source          1..1079
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(119..175)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(188..883)
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
                     KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                     HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR
                     TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF
                     KTPDADAGEE"
     regulatory      880..889
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(915..939)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(936..974)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    986..1004
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1022..1040
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1057..1075
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 ggcgccgctg cgtttcccgg aaccacgccc agagcagccg cgtccctgcg caaacccagg
       61 gctgccttgg aaaaggcgca accccaaccc cggatcctgt acaacgcgtc tgcagcctag
      121 gaccggggtt ttcttccacg tctcctgctt gctttaacag agagaagttc gtggctccgg
      181 aaccggtttc ttcaccggca tctgcatccg gggtcttgaa ggcgtgctgg tactccacga
      241 tgcccagctc ggtgttgctg tgatcctcct ccacgcggcg gaaggcgaac atggggcccc
      301 cgttctgcag gatgctgggg tggatggcgc tcttgaagtg catgtggctg tccaccacgg
      361 agctgtagta gccgccgtcg cgcaggctga aggtgcgggt gaagctgcca tccagatcgt
      421 tatcgcccat ggggtgcagg tgctccacgg tggcgttgct gcggatgatc ttgtcggtga
      481 agatcacgct gtcctcgggg aagccggtgc ccatcacctt gaagtcgccg atcacgcggc
      541 cggcctcgta gcggtagctg aagctcacgt gcagcacgcc gccgtcctcg tacttctcga
      601 tgcgggtgtt ggtgtagccg ccgttgttga tggcgtgcag gaaggggttc tcgtagccgc
      661 tggggtaggt gccgaagtgg tagaagccgt agcccatcac gtggctcagc aggtaggggc
      721 tgaaggtcag ggcgcctttg gtgctcttca tcttgttggt catgcggccc tgctcggggg
      781 tgccctctcc gccgcccacc agctcgaact ccacgccgtt cagggtgccg gtgatgcggc
      841 actcgatctc catggcgggc aggccgctct cgtcgctctc catggtggcg aattcgctag
      901 ccaattctcc aggcgatctg acggttcact aaacgagctc tgcttatata ggcctcccac
      961 cgtacacgcc tacctcgaca tacgttctct atcactgata gggagtaaac tcgacatacg
     1021 ttctctatca ctgataggga taaactcgac atacgttctc tatcactgat agggagtaa
//
