LOCUS       sequence-137376-         594 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA scaffold and mouse U6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Vidigal JA, Ventura A
  TITLE     Rapid and efficient one-step generation of paired gRNA CRISPR-Cas9 
            libraries.
  JOURNAL   Nat Commun. 2015 Aug 17;6:8083. doi: 10.1038/ncomms9083.
  PUBMED    26278926
REFERENCE   2  (bases 1 to 594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-08-17"; volume: "6:8083. doi"; pages: " 
            10.1038/ncomms9083"
COMMENT     Sequence Label: pDonor_mU6
FEATURES             Location/Qualifiers
     source          1..594
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        59..134
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        231..544
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     463..483
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 cgagctcgga tccactagta acggccgcca gtgtgctgga attcgccctt gaagacctgt
       61 tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg
      121 caccgagtcg gtgctttttt gttttagagc tagaaatagc aagttaaaat aaggctagtc
      181 cgtttttagc gcgtgcgcca attctgcaga caaatggctc tagatctaga gatccgacgc
      241 cgccatctct aggcccgcgc cggccccctc gcacagactt gtgggagaag ctcggctact
      301 cccctgcccc ggttaatttg catataatat ttcctagtaa ctatagaggc ttaatgtgcg
      361 ataaaagaca gataatctgt tctttttaat actagctaca ttttacatga taggcttgga
      421 tttctataag agatacaaat actaaattat tattttaaaa aacagcacaa aaggaaactc
      481 accctaactg taaagtaatt gtgtgttttg agactataaa tatgcatgcg agaaaagcct
      541 tgtttggttt gggtcttcaa gggcgaattc tgcagatatc catcacactg gcgg
//
