LOCUS       sequence-137380-         533 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA scaffold and synthetic U6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Vidigal JA, Ventura A
  TITLE     Rapid and efficient one-step generation of paired gRNA CRISPR-Cas9 
            libraries.
  JOURNAL   Nat Commun. 2015 Aug 17;6:8083. doi: 10.1038/ncomms9083.
  PUBMED    26278926
REFERENCE   2  (bases 1 to 533)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-08-17"; volume: "6:8083. doi"; pages: " 
            10.1038/ncomms9083"
COMMENT     Sequence Label: pDonor_sU6
FEATURES             Location/Qualifiers
     source          1..533
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        60..135
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     226..246
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 ccgagctcgg atccactagt aacggccgcc agtgtgctgg aattcgccct tgaagacctg
       61 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
      121 gcaccgagtc ggtgcttttt tgttttagag ctagaaatag caagttaaaa taaggctagt
      181 ccgtttttag cgcgtgcgcc aattctgcag acaaatggct ctagagaggg cctatttccc
      241 atgattcctt catatttgca tataatattt cctagtaact atagaggctt aatgtgcgat
      301 aaaagacaga taatctgttc tttttaatac tagctacatt ttacatgata ggcttggatt
      361 tctataagag atacaaatac taaattatta ttttaaaaaa cagcacaaaa ggaaacttac
      421 cgtaacttga aagtaattgt gtgttttgag actttatata tgcatgcgag aaaagccttg
      481 tttggtttgg gtcttcaagg gcgaattctg cagatatcca tcacactggc ggc
//
