LOCUS       sequence-137383-        8738 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the Contactin 2 protein 
            (truncated immediately upstream of propeptide cleavage and 
            GPI-attachement site), C-terminally fused to the Fc region of human 
            IgG1 + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8738)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 8738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Cntn2-Fc-His
FEATURES             Location/Qualifiers
     source          1..8738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      1604..1613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5318..5335
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    5361..5482
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5398..5417)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5452..5471
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5520..5537)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5691..6279)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5771..5790)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6450..7241,7242..7310))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7073..7092
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7311..7415)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7483..7501
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7539..7561)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7563..7583)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7568..7898
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7749..7884
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7811..7830
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8035..8054
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(8121..8576)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8258..8279)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8470..8489
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8702..8724
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8716..8733
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8717..8733
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tgggagcacc
     1621 ggccaggaaa agggcaagct tgctgctgct gctgctggcc acaatggctc tggtctcctc
     1681 tccaggatgg agtttttccc agggaacccc agctaccttt ggacccgtct ttgaagagca
     1741 acctgttggc ctgctattcc cagaggagtc tgcagaggat caggtgacac tggcgtgccg
     1801 tgcccgagct agccctccag ccacctatag gtggaagatg aacggcacag agatgaacct
     1861 ggaacctggt tcccgtcacc agctgatggg gggcaacctg gtcatcatga gtcccaccaa
     1921 agcacaggat gctggtgtct accagtgcct agcctctaac ccagtaggca ctgtcgtcag
     1981 caaggaagct gtcctccgct tcggctttct acaggaattc tccaaggagg agagagaccc
     2041 cgtgaaaacc cacgagggct ggggggtgat gctgccctgt aacccacctg cccactaccc
     2101 aggtttgtcc taccgctggc tcctcaacga gttccccaac ttcatcccaa cggatgggcg
     2161 tcacttcgtg tcccagacca cagggaacct gtacatcgcc cgcaccaatg cctcggacct
     2221 gggcaactac tcttgcctgg ctaccagcca cctggacttc tccaccaaga gcgtcttcag
     2281 caaattcgcg cagctcaacc tggctgctga agatcctcga ctctttgctc ccagtatcaa
     2341 agcccggttc cccccagaga cgtacgcact ggttgggcag caggtcaccc tggagtgctt
     2401 tgcctttggg aaccctgttc ccaggatcaa gtggcgcaaa gtggatggtt ccttgtcccc
     2461 tcagtggggc acagcagagc ccaccctgca gatccccagt gttagctttg aagatgaggg
     2521 tacctatgaa tgtgaggcag agaactccaa gggccgtgac accgtccagg gacgcatcat
     2581 tgtgcaagct cagcctgagt ggctaaaggt gatctcagac acagaagccg acattggttc
     2641 caacttacgt tggggctgtg ctgcggcagg caagcctcgg cccatggtgc gctggctgag
     2701 aaacggggaa cctctggcct cccagaaccg ggtggaggtc ttggctgggg acctgcgatt
     2761 ctctaagctg aacctggagg actccggcat gtaccagtgt gtggcagaaa acaagcatgg
     2821 caccatctat gccagtgctg agctggctgt acaagctctg gccccagact tcaggcagaa
     2881 ccctgtgagg cggctgatcc ctgcagcccg agggggagag atcagtatcc cgtgccagcc
     2941 ccgtgcggct ccaaaagcta caatactgtg gagcaagggt actgagattc tggggaacag
     3001 taccagagtg actgtcactt tggacggcac cttgattatc agaaacatca gtcgatcgga
     3061 tgaaggcaaa tatacctgct ttgctgagaa cttcatgggc aaagccaaca gtaccgggat
     3121 cctgtctgtg cgagatgcaa cgaagatcac gctagctcct tcgagtgctg acatcaatgt
     3181 gggtgataac ctgaccctac agtgccacgc ctcgcatgac cccactatgg acctcacgtt
     3241 cacctggacc ctggatgact tccctgttga ctttgataag cctggaggtc actaccggag
     3301 agccagtgtg aaggagacca ttggggacct gactatcctg aatgcccagc tacgccacgg
     3361 agggacatac acatgcatgg cccagacagt ggtggatggt gcatccaagg aggccacagt
     3421 cctggtccga ggtcccccag gtcctcctgg gggtgtggtg gtgagagaca ttggagacac
     3481 tactgttcag cttagctgga gtcgtggctt tgacaatcac agccccattg ccaagtacac
     3541 cctgcaagcg cggactccac cctcagggaa atggaagcag gttcggacca atcctgtgaa
     3601 tatcgagggc aacgcggaaa ctgcccaggt gctggggctc atgccttgga tggactatga
     3661 gtttcgggtt tcagctagca acatcttggg cactggggag cccagcggcc cctccagcag
     3721 aatccgcact aaggaagcag tcccctctgt ggcaccatct ggactcagtg gagggggagg
     3781 agcccctgga gagcttacca tcaactggac tcccatgtca cgagagtacc agaatggaga
     3841 cggcttcggc tacctgctgt ccttccgcag gcaaggcagc tccagttggc agacggcccg
     3901 ggtgcccggc gctgataccc agtacttcgt ctacagcaac gatagcatcc atccctacac
     3961 accctttgag gtcaagatcc gaagctacaa tcgccggggg gatgggcccg agagcctcac
     4021 tgccatcgtg tactcagcag aggaagagcc caaggtggcc cctgccaagg tctgggccaa
     4081 gggttcctca tcctcagaga tgaacgtaag ctgggagcct gtgctacaag acatgaacgg
     4141 catcctcctg ggatatgaga ttcgctactg gaaagccggg gacaaagaag ctgccgctga
     4201 ccgagtgagg acggcagggc tagactccag tgcccgagtc accggcctgt accccaacac
     4261 caagtaccat gtaactgtga gggcctacaa cagggctggc accggaccag ctagcccttc
     4321 agctgatgcc atgaccatga agcccccacc acggcgacct cctggcaaca tctcctggac
     4381 tttctcaagt tccagcctca gccttaagtg ggaccctgtg gttcctctcc gaaatgaatc
     4441 tacagtcact ggctacaaga tgttgtatca gaatgacctg cagccaactc ctatgctcca
     4501 cctcaccagc aagaactgga tagaaatacc agtacctgaa gacattggtc acgctctggt
     4561 gcagattcga accacggggc ctggagggga tgggatacca gccgaagtcc acattgtgag
     4621 aaatactagt gacaaaactc acacatgccc accgtgccca gcacctgaac tcctgggggg
     4681 accgtcagtc ttcctcttcc ccccaaaacc caaggacacc ctcatgatct cccggacccc
     4741 tgaggtcaca tgcgtggtgg tggacgtgag ccacgaagac cctgaggtca agttcaactg
     4801 gtacgtggac ggcgtggagg tgcataatgc caagacaaag ccgcgggagg agcagtacaa
     4861 cagcacgtac cgtgtggtca gcgtcctcac cgtcctgcac caggactggc tgaatggcaa
     4921 ggagtacaag tgcaaggtct ccaacaaagc cctcccagcc cccatcgaga aaaccatctc
     4981 caaagccaaa gggcagcccc gagaaccaca ggtgtatacc ctgcccccat cccgggatga
     5041 gctgaccaag aaccaggtca gcctgacctg cctggtcaaa ggcttctatc ccagcgacat
     5101 cgccgtggag tgggagagca atgggcagcc ggagaacaac tacaagacca cgcctcccgt
     5161 gctggactcc gacggctcct tcttcctcta cagcaagctc accgtggaca agagcaggtg
     5221 gcagcagggg aacgtcttct catgctccgt gatgcatgag gctctgcaca accactacac
     5281 gcagaagagc ctctccctgt ctccgggtaa aggcggccat catcaccatc accattaatc
     5341 tagagatctt attaaagcag aacttgttta ttgcagctta taatggttac aaataaagca
     5401 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     5461 ccaaactcat caatgtatct tatcatgtct ggtcgactct agctagactc ttccgcttcc
     5521 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     5581 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     5641 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     5701 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     5761 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     5821 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     5881 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     5941 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     6001 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     6061 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     6121 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     6181 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     6241 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     6301 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     6361 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     6421 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     6481 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     6541 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     6601 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     6661 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     6721 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     6781 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     6841 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     6901 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     6961 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     7021 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     7081 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     7141 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     7201 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     7261 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     7321 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     7381 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     7441 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     7501 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     7561 gaggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccaggca ggcagaagta
     7621 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     7681 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     7741 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     7801 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     7861 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     7921 ccattttcgt cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     7981 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     8041 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     8101 ggagaaaata ccgcatcagg aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     8161 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     8221 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     8281 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     8341 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     8401 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     8461 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     8521 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat
     8581 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     8641 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     8701 tcccagtcac gacgttgtaa aacgacggcc agtgaatt
//
