LOCUS       sequence-137388-         709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing FKBPF36V protein in washing out shield-1 ligand for 
            FKBPdd.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Egeler EL, Urner LM, Rakhit R, Liu CW, Wandless TJ
  TITLE     Ligand-switchable substrates for a ubiquitin-proteasome system.
  JOURNAL   J Biol Chem. 2011 Sep 9;286(36):31328-36. doi: 
            10.1074/jbc.M111.264101. Epub 2011 Jul 15.
  PUBMED    21768107
REFERENCE   2  (bases 1 to 709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.264101"; journalName: "J Biol Chem"; date: 
            "2011-09-9- 9"; volume: "286"; issue: "36"; pages: "31328-36"
COMMENT     Sequence Label: pET15b His6-FKBPF36V
FEATURES             Location/Qualifiers
     source          1..709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(18..341,342..344))
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             complement(357..377)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(384..401)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(411..428)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             447..469
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    484..508
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(508..527)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(509..527)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(611..630)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tgttagcagc cggatcctca ttccagtttt agaagctcca catcgaagac gagagtggca
       61 tgtggtggga tgatgcctgg gtgcccagtg gcaccatagg cataatctgg agatatagtc
      121 agtttggctc tctgacccac actcatctgg gcaacccctt cttcccagcc tcggatcacc
      181 tcctgcttgc ctagcataaa cttaaagggc ttgtttctgt cccgggagga atcaactttc
      241 tttccatctt caagcatccc ggtgtagtgc accacgcagg tctggccgcg cttggggaag
      301 gtgcgcccgt ctcctgggga gatggtttcc acctgcactc ccatggatcc tccacctccc
      361 tgaaaataca ggttttccat atggctgccg cgcggcacca ggccgctgct gtgatgatga
      421 tgatgatggc tgctgcccat ggtatatctc cttcttaaag ttaaacaaaa ttatttctag
      481 aggggaattg ttatccgctc acaattcccc tatagtgagt cgtattaatt tcgcgggatc
      541 gagatctcga tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg
      601 gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg
      661 ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccg
//
