LOCUS       sequence-137391-         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral Gateway destination vector, expression, CMV 
            promoter, Puro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti CMV Puro DEST (w118-1)
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(110..234)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(318..338)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(736..747)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
       61 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg
      121 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
      181 attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggttgatat
      241 ccagcacagt ggcggccgct cgacaatcaa cctctggatt acaaaatttg tgaaagattg
      301 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
      361 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
      421 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
      481 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
      541 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
      601 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
      661 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
      721 ttctgctacg tcccttcggc cctcaatcca gcggaccttc ct
//
