LOCUS       sequence-137394-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CEN plasmid as receiving vector for exogenous pathway.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: pRS414-URR
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             671..682
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    700..716
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(724..730,731..748,749..754))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    769..790
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 agtgacgacc ttcagcacgt tcgtactgtt caacgatggt gtagtcttcg ttgtgggagg
       61 tgatgtccag tttgatgtcg gttttgtaag cacccggcag ctgaaccggt tttttagcca
      121 tgtaggtggt tttaacttca gcgtcgtagt gaccaccgtc tttcagtttc agacgcattt
      181 tgatttcacc tttcagagca ccgtcttccg ggtacatacg ttcggtggaa gcttcccaac
      241 ccatggtttt tttctgcata accggaccgt cggacgggaa gttggtacca cgcagtttaa
      301 ctttgtagat gaactcaccg tcttgcaggg aggagtcctg ggtaacggta acaacaccac
      361 cgtcttcgaa gttcataaca cgttcccatt tgaaaccttc cgggaaggac agtttcaggt
      421 agtccgggat gtcagccggg tgtttaacgt aagctttgga accgtactgg aactgcgggg
      481 acaggatgtc ccaagcgaac ggcagcggac cacctttggt aactttcagt ttagcggtct
      541 gggtaccttc gtacggacga ccttcacctt caccttcgat ttcgaactcg tgaccgttaa
      601 cggaaccttc catacgaact ttgaaacgca tgaactcttt gataacgtct tcggaggaag
      661 ccatctagta tttctcctct ttctctagta tgtgtgaaat tgttatccgc tcacaattcc
      721 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
      781 actcacatta attgcgttgc gctcactgcc cgctttccag tcgg
//
