LOCUS       sequence-137405-         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible sucrose counter-selection allelic exchange vector
            for Pseudomonas syringae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Mijatovic J, Severns PM, Kemerait RC, Walcott RR, Kvitko BH
  TITLE     Patterns of Seed-to-Seedling Transmission of Xanthomonas citri pv. 
            malvacearum, the Causal Agent of Cotton Bacterial Blight.
  JOURNAL   Phytopathology. 2021 Dec;111(12):2176-2184. doi: 
            10.1094/PHYTO-02-21-0057-R. Epub 2021 Nov 22.
  PUBMED    34032522
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/PHYTO-02-21-0057-R"; journalName: "Phytopathology"; date: 
            "2021-12"; volume: "111"; issue: "12"; pages: "2176-2184"
COMMENT     Sequence Label: pDEST1K18ms
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    26..150
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(191..496)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(293..312)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 aggatccccc tcgggtatgc atggcacaag tttgtacaaa aaagctgaac gagaaacgta
       61 aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata
      121 ctgtaagaca caacatatcc agtcactatg gtcgacctgc agactggctg tgtataaggg
      181 agcctgacat ttatattccc cagaacatca ggttaatggc gtttttgatg tcattttcgc
      241 ggtggctgag atcagccact tcttccccga taacggagac cggcacactg gccatatcgg
      301 tggtcatcat gcgccagctt tcatccccga tatgcaccac cgggtaaagt tcacgggaga
      361 ctttatctga cagcagacgt gcactggcca gggggatcac catccgtcgc ccgggcgtgt
      421 caataatatc actctgtaca tccacaaaca gacgataacg gctctctctt ttataggtgt
      481 aaaccttaaa ctgcatttca ccagcccctg ttctcgtcag caaaagagcc gttcatttca
      541 ataaaccggg cgacctcagc catcccttcc tgattttccg ctttccagcg ttcggcacgc
      601 agacgacggg cttcattctg catggttgtg cttaccagac cggagatatt gacatcatat
      661 atgccttgag caactgatag ctgtcgctgt caactgtcac tgtaatacgc tgcttcatag
      721 catacctctt tttgacatac ttcgggtata catatcagta tatattctta taccgcaaaa
      781 atcagcgcgc aaatacgcat actgttatct ggctt
//
