LOCUS       sequence-137431-       11046 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mutated p53-L344A tagged with mKate2 and split N-term 
            mVenus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11046)
  AUTHORS   Gaglia G, Lahav G
  TITLE     Constant rate of p53 tetramerization in response to DNA damage 
            controls the p53 response.
  JOURNAL   Mol Syst Biol. 2014 Oct 24;10:753. doi: 10.15252/msb.20145168.
  PUBMED    25344068
REFERENCE   2  (bases 1 to 11046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-10-24"; volume: "10:753. doi"; pages: " 
            10.15252/msb.20145168"
COMMENT     Sequence Label: pRRLNeo-pEF1a-p53ashL344A-mKate2-splitmVenusN
FEATURES             Location/Qualifiers
     source          1..11046
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(132..200,201..992)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(350..369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1652..1671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1905..1922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2075..2080,2081..2098,2099..2105)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2118..2140
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2137..2153
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             2449..2629
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2676..2801
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3298..3531
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3716..3760
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4053..4170
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4210..4230
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4231..5409
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4462..5400
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5357..5377
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    5415..5439
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5442..6620
                     /codon_start=1
                     /gene="Homo sapiens TP53"
                     /product="human tumor suppressor protein that is often 
                     mutated in cancers"
                     /label=p53
                     /note="responds to cellular stresses to regulate the 
                     expression of target genes"
                     /translation="MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLML
                     SPDDIEQWFTEDPGPDEAPRMPEAAPRVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQ
                     GSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIY
                     KQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEP
                     PEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRD
                     RRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEM
                     FREANEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD"
     protein_bind    complement(6622..6646)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6648..7343
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     CDS             join(7371..7373,7374..7376,7377..7835)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITA"
     primer_bind     complement(7416..7437)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7677..7696)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     protein_bind    complement(7849..7869)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        7901..8257
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7901..8091
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7923..7943)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8108..8243
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8292..9086
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8292..9086
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8346..8365)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8956..8975
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    9128..9716
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9181..9201)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9599..9610)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9791..10024
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10096..10217
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10133..10152)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10187..10206
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10236..10371
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10403..10422)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10404..10422)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10432..10448)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10441..10463)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10589..11044
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10676..10695)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10886..10907
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaatttaa ccctcactaa agggaacaaa agctggagct gcaagcttaa
     2221 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
     2281 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
     2341 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
     2401 cgcattgcag agatattgta tttaagtgcc tagctcgata caataaacgg gtctctctgg
     2461 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     2521 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     2581 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     2641 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     2701 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     2761 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     2821 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     2881 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     2941 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     3001 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     3061 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     3121 agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag
     3181 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     3241 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     3301 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     3361 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     3421 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     3481 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     3541 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     3601 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     3661 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     3721 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     3781 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     3841 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     3901 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     3961 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     4021 ccgaattcac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     4081 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     4141 caaaaacaaa ttacaaaaat tcaaaatttt atcgataagc ttgatatcac catgattacg
     4201 ccaagctatc aactttgtat agaaaagttg ggctccggtg cccgtcagtg ggcagagcgc
     4261 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag
     4321 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     4381 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     4441 gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt
     4501 acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta cgtgattctt
     4561 gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc
     4621 ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc
     4681 tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt
     4741 tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat
     4801 ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag
     4861 cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag
     4921 tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc
     4981 tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc
     5041 ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag
     5101 tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg
     5161 gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct
     5221 ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact
     5281 gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt
     5341 ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag
     5401 gtgtcgtgag gaattcaagt ttgtacaaaa aagcaggctt catggaggag ccgcagtcag
     5461 atcctagcgt cgagccccct ctgagtcagg aaacattttc agacctatgg aaactacttc
     5521 ctgaaaacaa cgttctgtcc cccttgccgt cccaagcaat ggatgatttg atgctgtccc
     5581 cggacgatat tgaacaatgg ttcactgaag acccaggtcc agatgaagct cccagaatgc
     5641 cagaggctgc tccccgcgtg gcccctgcac cagcagctcc tacaccggcg gcccctgcac
     5701 cagccccctc ctggcccctg tcatcttctg tcccttccca gaaaacctac cagggcagct
     5761 acggtttccg tctgggcttc ttgcattctg ggacagccaa gtctgtgact tgcacgtact
     5821 cccctgccct caacaagatg ttttgccaac tggccaagac ctgccctgtg cagctgtggg
     5881 ttgattccac acccccgccc ggcacccgcg tccgcgccat ggccatctac aagcagtcac
     5941 agcacatgac ggaggttgtg aggcgctgcc cccaccatga gcgctgctca gatagcgatg
     6001 gtctggcccc tcctcagcat cttatccgag tggagggaaa tttgcgtgtg gagtatttgg
     6061 atgacagaaa cacttttcga catagtgtgg tggtgcccta tgagccgcct gaggttggct
     6121 ctgactgtac caccatccac tacaactaca tgtgtaacag ttcctgcatg ggcggcatga
     6181 accggaggcc catcctcacc atcatcacac tggaggatag ctctggaaac ctactgggac
     6241 ggaacagctt tgaggtgcgt gtttgtgcct gtcctgggag agaccggcgc acagaggaag
     6301 agaatctccg caagaaaggg gagcctcacc acgagctgcc cccagggagc actaagcgag
     6361 cactgcccaa caacaccagc tcctctcccc agccaaagaa gaaaccactg gatggagaat
     6421 atttcaccct tcagatccgt gggcgtgagc gcttcgagat gttccgagag gcgaatgagg
     6481 ccttggaact caaggatgcc caggctggga aggagccagg ggggagcagg gctcactcca
     6541 gccacctgaa gtccaaaaag ggtcagtcta cctcccgcca taaaaaactc atgttcaaga
     6601 cagaagggcc tgactcagac gacccagctt tcttgtacaa agtgggaatg gtgtctgagc
     6661 tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtgaac aaccaccact
     6721 tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca
     6781 aggccgtcga gggcggccct ctccccttcg ccttcgacat cctggctacc agcttcatgt
     6841 acggcagcaa aaccttcatc aaccacaccc agggcatccc cgacttcttt aagcagtcct
     6901 tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg
     6961 ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg
     7021 tgaacttccc atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggcctcca
     7081 ccgagaccct gtaccccgct gacggcggcc tggaaggcag agccgacatg gccctgaagc
     7141 tcgtgggcgg gggccacctg atctgcaact tgaagaccac atacagatcc aagaaacccg
     7201 ctaagaacct caagatgccc ggcgtctact atgtggacag aagactggaa agaatcaagg
     7261 aggccgacaa agagacctac gtcgagcagc acgaggtggc tgtggccaga tactgcgacc
     7321 tccctagcaa actggggcac agaggaggag gtggaggtgg aggtggggga atggtgagca
     7381 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     7441 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     7501 ccctgaagct gatctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     7561 ccctgggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact
     7621 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     7681 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     7741 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     7801 acaactacaa cagccacaac gtctatatca ccgccgacaa gcagtaatca actttattat
     7861 acatagttga taattcactg gccgtcgaga tccggactag tgaggcggaa agaaccagct
     7921 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     7981 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     8041 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     8101 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     8161 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     8221 gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag
     8281 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     8341 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     8401 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     8461 tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     8521 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     8581 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     8641 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     8701 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     8761 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga
     8821 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     8881 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     8941 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     9001 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     9061 atcgccttct tgacgagttc ttctgagcga ctagttaacg cgtcgaccag ctagctggcc
     9121 agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     9181 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     9241 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     9301 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     9361 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     9421 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     9481 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     9541 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     9601 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     9661 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctga
     9721 gatcctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     9781 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     9841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     9901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     9961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
    10021 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
    10081 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
    10141 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    10201 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt
    10261 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
    10321 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
    10381 gcgtcgagac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
    10441 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
    10501 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
    10561 acagttgcgc agcctgaatg gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg
    10621 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
    10681 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
    10741 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
    10801 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
    10861 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
    10921 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
    10981 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
    11041 atttcc
//
