LOCUS       sequence-137438-         705 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  reporter plasmids used in YeastFab work, in which promoters could be
            inserted just upstream YFP(YEVenus) to drive its expression, also 
            contains an mCherry gene driven by TEF2 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: pYG026
FEATURES             Location/Qualifiers
     source          1..705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      250..437
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(305..329)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa
       61 gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa
      121 gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga
      181 gggccgccac tccaccggcg gcatggacga gctgtacaag taacggaccg gcgcgccact
      241 tctaaataag cgaatttctt atgatttatg atttttatta ttaaataagt tataaaaaaa
      301 ataagtgtat acaaatttta aagtgactct taggttttaa aacgaaaatt cttattcttg
      361 agtaactctt tcctgtaggt caggttgctt tctcaggtat agtatgaggt cgctcttatt
      421 gaccacacct ctaccggcac ccgggttagt tttgctggcc gcatcttctc aaatatgctt
      481 cccagcctgc ttttctgtaa cgttcaccct ctaccttagc atcccttccc tttgcaaata
      541 gtcctcttcc aacaataata atgtcagatc ctgtagatac cacatcatcc acggttctat
      601 actgttgacc caatgcgtct cccttgtcat ctaaacccac accgggtgtc ataatcaacc
      661 aatcgtaacc ttcatctctt ccacccatgt ctctttgagc aataa
//
