LOCUS       sequence-137442-         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral transfer vector for tet-inducible expression of EGFP 
            tagged human RHOA-T19N.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Kong YP, Carrion B, Singh RK, Putnam AJ
  TITLE     Matrix identity and tractional forces influence indirect cardiac 
            reprogramming.
  JOURNAL   Sci Rep. 2013 Dec 11;3:3474. doi: 10.1038/srep03474.
  PUBMED    24326998
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013-12-11"; volume: "3:3474. doi"; pages: " 
            10.1038/srep03474"
COMMENT     Sequence Label: tetO-FUW-eGFP-RHOA-T19N
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(62..64,65..67,68..778)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(62..64,65..67,68..778)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(107..128)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(368..387)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     715..736
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ttttgacctc catagaagac accgggaccg atccagcctc cgcggccccg aattctctag
       61 aatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
      121 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
      181 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
      241 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
      301 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
      361 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
      421 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
      481 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
      541 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
      601 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
      661 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
      721 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagga
      781 attcatggct gccatccgga agaaactggt gattgttggt gatggagcct gtggaaagaa
      841 ctgcttgctc atagtcttca gcaaggacca g
//
