LOCUS       sequence-137449-         662 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of human DNA Polymerase beta (POLB).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Schuermann D, Scheidegger SP, Weber AR, Bjoras M, Leumann CJ, Schar 
            P
  TITLE     3CAPS - a structural AP-site analogue as a tool to investigate DNA 
            base excision repair.
  JOURNAL   Nucleic Acids Res. 2016 Jan 4. pii: gkv1520.
  PUBMED    26733580
REFERENCE   2  (bases 1 to 662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Jan 4. pii: gkv1520."
COMMENT     Sequence Label: pQE30-hPolB
FEATURES             Location/Qualifiers
     source          1..662
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    1..17
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    33..49
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             68..79
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             98..115
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttgtgagcgg ataacaatta taatagattc aattgtgagc ggataacaat ttcacacaga
       61 attcattaaa gaggagaaat taactatgag aggatcgcat caccatcacc atcacggatc
      121 ctctaaacgg aaggcgccgc aggagactct caacggggga atcaccgaca tgctcacaga
      181 actcgcaaac tttgagaaga acgtgagcca agctatccac aagtacaatg cttacagaaa
      241 agcagcatct gttatagcaa aatacccaca caaaataaag agtggagctg aagctaagaa
      301 attgcctgga gtaggaacaa aaattgctga aaagattgat gagtttttag caactggaaa
      361 attacgtaaa ctggaaaaga ttcggcagga tgatacgagt tcatccatca atttcctgac
      421 tcgagttagt ggcattggtc catctgctgc aaggaagttt gtagatgaag gaattaaaac
      481 actagaagat ctcagaaaaa atgaagataa attgaaccat catcagcgaa ttgggctgaa
      541 atattttggg gactttgaaa aaagaattcc tcgtgaagag atgttacaaa tgcaagatat
      601 tgtactaaat gaagttaaaa aagtggattc tgaatacatt gctacagtct gtggcagttt
      661 ca
//
