LOCUS       sequence-137452-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses tRNA and synthetase for mammalian encoding of a photocaged
            cysteine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Ren W, Ji A, Ai HW
  TITLE     Light activation of protein splicing with a photocaged fast intein.
  JOURNAL   J Am Chem Soc. 2015 Feb 18;137(6):2155-8. doi: 10.1021/ja508597d. 
            Epub 2015 Feb 5.
  PUBMED    25647354
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja508597d";
            journalName: "J Am Chem Soc"; date: "2015-02-18- 18"; volume: "137";
            issue: "6"; pages: "2155-8"
COMMENT     Sequence Label: pMAH2-CageCys
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        221..239
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     221..238
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     252..271
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg
       61 accgatccag cctccgcggc cgggaacggt gcattggaac gcggattccc cgtgccaaga
      121 gtgacgtaag taccgcctat agagtctata ggcccacccc cttggcttcg ttagaacgcg
      181 gctacaatta atacataacc ttatgtatca tacacatacg atttaggtga cactatagaa
      241 taacatccac tttgcctttc tctccacagg tgtccactcc caggtccaac tgcacggaag
      301 cttgccacca tgcaagaaca atatcggcct gaagaaattg aaagcaaggt tcagctccat
      361 tgggacgaga agaggacatt tgaagtgacc gaggatgagt caaaagaaaa gtactattgc
      421 ttgagtggac agccctaccc cagcggtcgc cttcacatgg gtcatgttcg gaattataca
      481 atcggggacg ttattgcgcg ctaccaaagg atgcttggaa agaacgtact ccaaccgatt
      541 ggctgggacg ccttcggctt gccagcagaa ggggcggctg tgaagaacaa tacagctcca
      601 gccccttgga cttatgacaa tatagcctac atgaaaaatc aactaaagat gctaggtttt
      661 ggatatgatt ggagtcgaga gttggcgact tgtacccctg aatactatcg ctgggaacaa
      721 aaatttttca ctgaactcta taagaaagga ctggtctaca aaaagacttc ggctgtcaat
      781 tggtgtccta acgatcaaac cgttctcgca a
//
