LOCUS       sequence-137465-        2245 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  construction of TEF2 promoter in HCKan-P receiving vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: HCKan-P-TEF2
FEATURES             Location/Qualifiers
     source          1..2245
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      553..584
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        585..687
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             688..1503
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(761..780)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1527..1554
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1566..2153)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1646..1665)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2175..2204)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 ggctgggcgc cataaccaag gtatctatag accgccaatc agcaaactac ctccgtacat
       61 tcatgttgca cccacacatt tatacaccca gaccgcgaca aattacccat aaggttgttt
      121 gtgacggcgt cgtacaagag aacgtgggaa ctttttaggc tcaccaaaaa aggaagaaaa
      181 aatacgagtt gctgacagaa gcctcaagaa aaaaaaaatt cttcttcgac tatgctggag
      241 gcagagatga tcgagccggt agttaactat atatagctaa attggttcca tcaccttctt
      301 ttctggtgtc gctccttcta gtgctatttc tggcttttcc tatttttttt tttccatttt
      361 tctttctctc tttctaatat ataaattctc ttgcattttc tatttttctc tctatctatt
      421 ctacttgttt attcccttca aggttttttt ttaaggagta cttgttttta gaatatgcgg
      481 tcaacgaact ataattaact aaagatgcga gacgactgac catttaaatc atacctgacc
      541 tccatagcag aaagtcaaaa gcctccgacc ggaggctttt gacttgatcg gcacgtaaga
      601 ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttatcg
      661 agattttcag gagctaagga agctaaaatg agccatattc aacgggaaac gtcttgctcg
      721 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat
      781 aatgtcgggc aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag
      841 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcagg
      901 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct
      961 gatgatgcat ggttactcac cactgcgatc ccagggaaaa cagcattcca ggtattagaa
     1021 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg
     1081 cattcgattc ctgtttgtaa ttgtcctttt aacggcgatc gcgtatttcg tctcgctcag
     1141 gcgcaatcac gaatgaataa cggtttggtt ggtgcgagtg attttgatga cgagcgtaat
     1201 ggctggcctg ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat
     1261 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta
     1321 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
     1381 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat
     1441 ggtattgata atcctgatat gaataaattg cagtttcact tgatgctcga tgagtttttc
     1501 taatgagggc ccaaatgtaa tcacctggct caccttcggg tgggcctttc tgcgttgctg
     1561 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgatg ctcaagtcag
     1621 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     1681 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     1741 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     1801 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     1861 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     1921 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     1981 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     2041 gttacctcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     2101 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     2161 ctttgatttt ctaccgaaga aaggcccacc cgtgaaggtg agccagtgag ttgattgcag
     2221 tccagttacg ctggagtccg tctcg
//
