LOCUS       sequence-137468-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  construction of ADH1 terminator in HCKan-P receiving vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: HCKan-T-ADH1
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      70..256
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(124..148)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     terminator      306..337
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        338..440
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(514..533)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 aaggcccacc cgtgaaggtg agccagtgag ttgattgcag tccagttacg ctggagtccg
       61 tctcgtagcc gaatttctta tgatttatga tttttattat taaataagtt ataaaaaaaa
      121 taagtgtata caaattttaa agtgactctt aggttttaaa acgaaaattc ttattcttga
      181 gtaactcttt cctgtaggtc aggttgcttt ctcaggtata gcatgaggtc gctcttattg
      241 accacacctc taccggcctc cgagacgact gaccatttaa atcatacctg acctccatag
      301 cagaaagtca aaagcctccg accggaggct tttgacttga tcggcacgta agaggttcca
      361 actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt
      421 caggagctaa ggaagctaaa atgagccata ttcaacggga aacgtcttgc tcgaggccgc
      481 gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg
      541 ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc
      601 tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc aggctaaact
      661 ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg
      721 catggttact caccactgcg atcccaggga aaacagcatt ccaggtatta gaagaatatc
      781 ctgattcagg tgaaa
//
