LOCUS       sequence-137474-        9618 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFPo_PH pCFJ150 - contains C. elegans sequence optimized GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9618)
  AUTHORS   Heppert JK, Dickinson DJ, Pani AM, Higgins CD, Steward A, Ahringer 
            J, Kuhn JR, Goldstein B
  TITLE     Comparative assessment of fluorescent proteins for in vivo imaging 
            in an animal model system.
  JOURNAL   Mol Biol Cell. 2016 Nov 7;27(22):3385-3394. doi: 
            10.1091/mbc.E16-01-0063. Epub 2016 Jul 6.
  PUBMED    27385332
REFERENCE   2  (bases 1 to 9618)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E16-01-0063"; journalName: "Mol Biol Cell"; date: 
            "2016-11-7- 7"; volume: "27"; issue: "22"; pages: "3385-3394"
COMMENT     Sequence Label: JH01
FEATURES             Location/Qualifiers
     source          1..9618
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..21
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             1941..1964
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1980..2003
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      2015..2338
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(3917..3936)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4036..4058
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4096..4114)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4182..4286
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4287..4355,4356..5147)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4505..4524)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5318..5906
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5807..5826
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7542..7553)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 caactttgta tagaaaagtt gaaatatcag tttttaaaaa attaaaccat aaaacaaata
       61 atataaccca atttttacat caaaccacaa gaaaaaaata catttgggcc cacggataaa
      121 gaaattaaaa aaatacattt tttaaaggcg caccgaatta aaattcattt gggtcttacc
      181 gcgtataccg tactccgttt gtttgatcat ttttgtcagc gctggcggtt gttttttcat
      241 ttcatttctg cttcaaagac gttttctcga ataatttttc gtttattctc ttttttaaaa
      301 ttaatttcta gccgtaaatg ttataaattc acccatttaa cgcaaatttc atggtaatct
      361 catggaaaaa tgcagtttct ttgttaaaga aagcttaaat agcaaaaatt ccccgacttt
      421 ccccaaaatc ctgctcgatt ttccgttttc tcattgtatt ctctcttaat taattttatc
      481 gataatcaat tgaatgtttc agacagagaa tgagtaaagg agaagaattg ttcactggag
      541 ttgtcccaat cctcgtcgag ctcgacggag acgtcaacgg acacaagttc tccgtctccg
      601 gagagggaga gggagacgcc acctacggaa agctcaccct caagttcatc tgcaccaccg
      661 gaaagctccc agtcccatgg ccaaccctcg tcaccacctt ctgctacgga gtccaatgct
      721 tctcccgtta cccagaccac atgaagcgtc acgacttctt caagtccgcc atgccagagg
      781 gatacgtcca agagcgtacc atcttcttca aggtaagttt aaacatatat atactaacta
      841 ctgattattt aaattttcag gacgacggaa actacaagac ccgtgccgag gtcaagttcg
      901 agggagacac cctcgtcaac cgtatcgagc tcaaggtaag tttaaacagt tcggtactaa
      961 ctaaccatac atatttaaat tttcagggaa tcgacttcaa ggaggacgga aacatcctcg
     1021 gacacaagct cgagtacaac tacaactccc acaacgtcta catcatggcc gacaagcaaa
     1081 agaacggaat caaggtcaac ttcaaggtaa gtttaaacat gattttacta actaactaat
     1141 ctgatttaaa ttttcagatc cgtcacaaca tcgaggacgg atccgtccaa ctcgccgacc
     1201 actaccaaca aaacacccca atcggagacg gaccagtcct cctcccagac aaccactacc
     1261 tctccaccca atccgccctc tccaaggacc caaacgagaa gcgtgaccac atggtcctcc
     1321 tcgagttcgt caccgccgcc ggaatcaccc acggaatgga cgagctctac aaggccggcc
     1381 acggactcca agacgaccca gacctccaag ccctcctcaa gggatcccaa ctcctcaagg
     1441 tcaagtcctc ctcctggcgt cgtgagcgtt tctacaagct ccaagaggac tgcaaggtaa
     1501 gtttaaacat atatatacta actaaccctg attatttaaa ttttcagacc atctggcaag
     1561 agtcccgtaa ggtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc
     1621 aggtcatgcg ttccccagag tcccaactct tctccatcga ggacatccaa gaggtccgta
     1681 tgggacaccg taccgaggga ctcgagaagt tcgcccgtga catcccagag gaccgttgct
     1741 tctccatcgt cttcaaggta agtttaaaca tgattttact aactaactaa tctgatttaa
     1801 attttcagga ccaacgtaac accctcgacc tcatcgcccc atccccagcc gacgcccaac
     1861 actgggtcca aggactccgt aagatcatcc accactccgg atccatggac caacgtcaaa
     1921 agtcaggagc tagcggagcc gattacaagg atgacgatga caagagagga tctggatctg
     1981 actacaagga cgatgacgat aagcgttaag ataaatgcaa aatcctttca agcattccct
     2041 tcttctctat cactcttctt tctttttgtc aaaaaattct ctcgctaatt tatttgcttt
     2101 tttaatgtta ttattttatg actttttata gtcactgaaa agtttgcatc tgagtgaagt
     2161 gaatgctatc aaaatgtgat tctgtctgat gtactttcac aatctctctt caattccatt
     2221 ttgaagtgct ttaaacccga aaggttgaga aaaatgcgag cgctcaaata tttgtattgt
     2281 gttcgttgag tgacccaaca aaaagaggaa actttattgt gccgccaaga aaaaagtctc
     2341 acgtacggtg cgcgcgatgc cgtatcacaa ctgagaaaaa agacaaaact tattgaacag
     2401 tgttcctttc aaaaaattat tttgtaaaaa atcgagtttt tctccaaaat aacgcacgca
     2461 atcaaaaaca agaaaactaa cgtgttctcc tgggcgtgga actccttatc aagattacca
     2521 ttattgtctg tcagaattga tatttcaatt atttcatatc tagacaaacc cgggtgtctt
     2581 gcatttatta gtactgtgag tttacataca aacgaggctt actcagataa catagagtca
     2641 cctctaatta gcaaaaaccc atttctaatt agctatttcg accagttcta aagtcaccgt
     2701 aattagaaat ggtttttgta gcttttctaa ttagaaatga ccccgagata agtgaaatat
     2761 ggcattttct gggttttcta gtaagaaatg atcccgcacg tagtgattcc gcgttacaag
     2821 ccaaattcaa attaaaaaag tatattacac aattcaagtt acattatttt caggaaacaa
     2881 tggcgaagag caaagcagag attcaaaagg aggaaagaga caacagatat gaacgaagac
     2941 ttcttgaaaa gagaaagaga catgaggtga gtaaccatga taataaataa tcaagtaagg
     3001 gctatagatt tataaattct ggagttaaca gtttcgaaaa aaagtagttt tcatatgtgc
     3061 gaaggaatat caaaaatagg ggaatagcct aaacgagtga gaaaattaaa tgtttgttat
     3121 tttttcaaaa atgtctgcag ggccgttatc ctttcaaatg cactctatca aacatagatc
     3181 atatccatca tatcttatcc agaatttata tcatatccaa gattgagagc gtttcaagga
     3241 aaatctcaaa caaccataca attttgtaga tcgacaacaa ggcctccact tcttcatcga
     3301 tggcctctgc agcacctgca ccaccaagtt tgtatttttc atattaatat aaagtgagcc
     3361 acgcatagtc gtttttagga tttttgccag atcgatgtcg cgcaaacaga ctgttttttt
     3421 ttctttgaaa gccgtgcgtg acatgggcca cggattgtaa cgccgcgctg gatggataaa
     3481 aatttattca gttttttagc ctttacaaaa tatggttcga ctactttctt tatttttaca
     3541 tttttatagg tgctcattga gctgagatat tttgggtata atcggaaaat cgctgatttt
     3601 ggtcattttt ctgcactaaa gattacacaa aaagttcagt atttagattc attgttgaaa
     3661 attggggatg atctacagat acatatcgcc gtgttcaaaa acacacgtga cgtcgtgact
     3721 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     3781 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     3841 gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     3901 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     3961 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     4021 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     4081 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     4141 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     4201 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     4261 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     4321 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     4381 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     4441 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     4501 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     4561 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     4621 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     4681 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     4741 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     4801 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     4861 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     4921 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     4981 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     5041 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     5101 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     5161 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     5221 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     5281 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     5341 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     5401 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     5461 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     5521 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     5581 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     5641 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     5701 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     5761 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     5821 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     5881 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     5941 tcttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     6001 accgtattac cgcctttgag tgagctgata ccagcggata acaatttcac agggccctgt
     6061 cggattatgg gctcttcttt tatcctctac tttctttcta gaaaatgcaa atgttgagtc
     6121 agaaattgct gaatggagta caactgcaat gggtttagct tatgtttctg gaacaattac
     6181 tggtggaatt gtgattgaaa agtaaggtta tatttcattt caataaatgt taccacagta
     6241 taacaaaaac aagttacatt gttcagagta tgtctgaatg tattattatt attgtatttg
     6301 tgcatcagta ctgtagttgt actgaagaag ggtttaaaat aatctcaacg tttttaagac
     6361 gagttagtga gaatacggtc gcagttagga tgtttggaca tagttttatt agataatcgt
     6421 gaactaactc actataaaaa cccgctaact cattgaataa gatagtttta aacgttcaaa
     6481 atttgaagac aaaattgtat gaataagttt acagagttgg tcgacgcaag cttctccttc
     6541 tcttcacatt tctcaataat ttagctctaa ttgcattcgt attcttcgca aaaatacgta
     6601 ttctgattga cccgatgaaa tacggatgcc tagttgcatt gatcatctac ggttatactt
     6661 atgggtgagg tttttcaaat atattttgat ttaatcgaat aatgaaattt cagaactgga
     6721 gttggtccaa tttcgtggtt tatatcctct gaactggtac ctcaaaaaca tcgtagtatt
     6781 gctcaatctg tggcatattc aatgaatact gtaatggtgg ttatcagtac atttacagta
     6841 ttacctctat attccgttat tggtagttat gctttcctta ttctatattc aattccttca
     6901 tttataagta tgcttatatt atttcgctac ttaccggaaa ccaaaggacg agagattcat
     6961 gatattgtga atgaacttaa aaaaaaatga aaacctttga aaacgttcat acggccgaaa
     7021 ttttacttga tcgagccaat tgaacttttg ttaagactta cagaaaagtg agagattttg
     7081 ttacaaaaaa gtgagaaaaa agcgaaaatg aacaggaaaa agaagaaaaa tacacccgaa
     7141 gtatcatata aaaattaaaa agattgtttg acctggcgga acttttttgc aaaaactctg
     7201 aatatccatg gcacatgctt cgtgcaaaac aggatccaaa tgaatatcaa caagagattc
     7261 ttgaagacga cgagccactt gctcagagca ttgcttatct cgaatgagac ccttgttaaa
     7321 atcatttttc aaacattata tgacattgtc gaaatgtcct cctgattcca tgatggtagc
     7381 aaactcactt cgtctttgag ccaatttatc caagtccttg taaaaagtat tcgaaaattg
     7441 ttaacggata aaatgtttat tataatatca aaaacaattt gtcagttgac cactttttga
     7501 tatatttgac agaaaccggg atgaattggc tcaaagttag ggcgcccttc tattacaggg
     7561 tttctgataa caaaccggtt attaactccc aacaagggat gatttcaatt catcatgctc
     7621 aaattgaccc aaattaagtt acatgacaaa ttcatcgccc ttttcaactc ttcttggctc
     7681 atcatctgtt attctgttcc tattctgtct gcaccctata cccctttgca tactctctcg
     7741 tcattccttt cttggatagt gtcttcttct ccccagctct gctacttcta tgacttgcgc
     7801 ccgctgcttt tccgctcgtt cctctcctct ctgacgtctt cttctgcttc ttttctcctt
     7861 cctttttccc ttctcaatct tcctcttttt cccatttctg tcaatcattc gaagaagaag
     7921 aagaagaaga ccctcattca tttcattttt ttttctgtcg gtgtgtgctg ctcggttaag
     7981 agtgagctct ctattccacg tcttcttctt tttcttcttc gattcgaatc aatcactcca
     8041 ccaaaacgca ttcgtttttg ggattcaccc cgcggttcgc aataggtttc tttttcaaat
     8101 tattagcgtt ataaatagaa aaatggtgga gtttcaataa aaatgataat ttacaaagtg
     8161 tattttgatt acatgtactc aaaaaggttg aaatttttca taccagtttt ccggaaatcc
     8221 atctgatatc attatcgtat ttttcttttt aaaaatgttt tcaaaaaaaa acaaaatata
     8281 gctggttatt tggcaccctc taattaccat tttcctgtca caccacactc ttttccttct
     8341 tccacttctt tccgttttca gccgcttcca accaaaccga tatgaaagcc gagcaacaac
     8401 gatcgattcc acccggctcg gcgaccttcc cgtcgcaggt gagactcaga aaactagaga
     8461 aaccgctcaa ctaactcttg atatccgatt tcattctttt ctcttttctt tttgttgaac
     8521 ttttccatat ttccagatgc cacggccacc accaagcacc gaacaaggaa tcacaacgga
     8581 atcggagctt gcgaagaaag ctcaaatcac tccgaacgac gttttagcac ttccgggaat
     8641 cactcaaggt atcttgctcc ctttggattt cagtttaaaa cataaattta ggattcttat
     8701 gctccccatc tgcgaacatc tataacatcg agttcaccaa gttccaaatc cgtgatctgg
     8761 acactgagca agtgcttttc gagatcgcca aaccggagaa cgatcaggag aatgatgagt
     8821 cgccacagga gtcggcaaga tacgtgcgtt atagatttgc tccaaacttt ttgaaactca
     8881 aaacggtcgg agcaactgtg gaattcaaag taggagacat cccaatccat catttccgaa
     8941 tgatcgaacg tcacttcttc aaagatcgcc ttctgaagtg ttttgacttt gaattcggat
     9001 tctgtattcc gaattcacga aacaactgtg aacatatcta tgagttccct caactctctc
     9061 aacaactcag tgagtcatta ttctaaaaag tacaattcaa aagactaatc tctttttcag
     9121 tggacgacat gatcaacaat ccaaacgaga ctcgttctga cagcttctat ttcgtcgata
     9181 acaaactcgt catgcacaac aaagccgact actcatatga tgcataaata tttaatacaa
     9241 aaatgttctg gataattatt ctgtcgaata gaaaaaaaac tccaaatgtg attaaattcc
     9301 aataattcct gtctagtttg ttccttcccc ttccccttct catgttcaat gcattcctaa
     9361 gcttttcagt tccccccttg tttctatatt ttttcgctgt cctgtcacac tcgctaaaaa
     9421 cactaatcac acggaaatct gttttcaata aaaactccaa ctttaactca ttttcaattt
     9481 caactgaaag attttttcat tagagaatgt ctagaactag gcccgggcta cgtaatacga
     9541 ctcacttaag gccttgacta gagggtacca gagctcacct aggcaggaac agctatgacc
     9601 atgattacgc caagctat
//
