LOCUS       sequence-137487-        9615 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mCherryo_PH pCFJ150 - contians C. elegans sequence optimized 
            mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9615)
  AUTHORS   Heppert JK, Dickinson DJ, Pani AM, Higgins CD, Steward A, Ahringer 
            J, Kuhn JR, Goldstein B
  TITLE     Comparative assessment of fluorescent proteins for in vivo imaging 
            in an animal model system.
  JOURNAL   Mol Biol Cell. 2016 Nov 7;27(22):3385-3394. doi: 
            10.1091/mbc.E16-01-0063. Epub 2016 Jul 6.
  PUBMED    27385332
REFERENCE   2  (bases 1 to 9615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E16-01-0063"; journalName: "Mol Biol Cell"; date: 
            "2016-11-7- 7"; volume: "27"; issue: "22"; pages: "3385-3394"
COMMENT     Sequence Label: JH02
FEATURES             Location/Qualifiers
     source          1..9615
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..21
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             1938..1961
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1977..2000
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      2012..2335
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(3914..3933)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4033..4055
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4093..4111)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4179..4283
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4284..4352,4353..5144)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4502..4521)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5315..5903
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5804..5823
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7539..7550)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 caactttgta tagaaaagtt gaaatatcag tttttaaaaa attaaaccat aaaacaaata
       61 atataaccca atttttacat caaaccacaa gaaaaaaata catttgggcc cacggataaa
      121 gaaattaaaa aaatacattt tttaaaggcg caccgaatta aaattcattt gggtcttacc
      181 gcgtataccg tactccgttt gtttgatcat ttttgtcagc gctggcggtt gttttttcat
      241 ttcatttctg cttcaaagac gttttctcga ataatttttc gtttattctc ttttttaaaa
      301 ttaatttcta gccgtaaatg ttataaattc acccatttaa cgcaaatttc atggtaatct
      361 catggaaaaa tgcagtttct ttgttaaaga aagcttaaat agcaaaaatt ccccgacttt
      421 ccccaaaatc ctgctcgatt ttccgttttc tcattgtatt ctctcttaat taattttatc
      481 gataatcaat tgaatgtttc agacagagaa tggtcagcaa gggagaggag gataatatgg
      541 caatcatcaa ggagttcatg cgtttcaagg tccacatgga gggatccgtc aacggacacg
      601 agttcgagat cgagggagag ggagagggac gtccatacga gggaacccaa accgccaagc
      661 tcaaggtcac caaggtaagt ttaaacatat atatactaac taaccctgat tatttaaatt
      721 ttcagggagg accactccca ttcgcctggg acatcctctc cccacaattc atgtacggat
      781 ccaaggccta cgtcaagcac ccagccgaca tcccagacta cctcaagctc tccttcccag
      841 agggattcaa gtgggagcgt gtcatgaact tcgaggacgg aggagtcgtc accgtcaccc
      901 aagactcctc cctccaagac ggagagttca tctacaaggt aagtttaaac agttcggtac
      961 taactaacca tacatattta aattttcagg tcaagctccg tggaaccaac ttcccatccg
     1021 acggaccagt catgcaaaag aagaccatgg gatgggaggc ctcctccgag cgtatgtacc
     1081 cagaggacgg agccctcaag ggagagatca agcaacgtct caagctcaag gtaagtttaa
     1141 acatgatttt actaactaac taatctgatt taaattttca ggacggagga cactacgacg
     1201 ccgaggtcaa gaccacctac aaggccaaga agccagtcca actcccagga gcctacaacg
     1261 tcaacatcaa gctcgacatc acctcccaca acgaggacta caccatcgtc gagcaatacg
     1321 agcgtgccga gggacgtcac tccaccggag gaatggacga gctctacaag gccggccacg
     1381 gactccaaga cgacccagac ctccaagccc tcctcaaggg atcccaactc ctcaaggtca
     1441 agtcctcctc ctggcgtcgt gagcgtttct acaagctcca agaggactgc aaggtaagtt
     1501 taaacatata tatactaact aaccctgatt atttaaattt tcagaccatc tggcaagagt
     1561 cccgtaaggt aagtttaaac agttcggtac taactaacca tacatattta aattttcagg
     1621 tcatgcgttc cccagagtcc caactcttct ccatcgagga catccaagag gtccgtatgg
     1681 gacaccgtac cgagggactc gagaagttcg cccgtgacat cccagaggac cgttgcttct
     1741 ccatcgtctt caaggtaagt ttaaacatga ttttactaac taactaatct gatttaaatt
     1801 ttcaggacca acgtaacacc ctcgacctca tcgccccatc cccagccgac gcccaacact
     1861 gggtccaagg actccgtaag atcatccacc actccggatc catggaccaa cgtcaaaagt
     1921 caggagctag cggagccgat tacaaggatg acgatgacaa gagaggatct ggatctgact
     1981 acaaggacga tgacgataag cgttaagata aatgcaaaat cctttcaagc attcccttct
     2041 tctctatcac tcttctttct ttttgtcaaa aaattctctc gctaatttat ttgctttttt
     2101 aatgttatta ttttatgact ttttatagtc actgaaaagt ttgcatctga gtgaagtgaa
     2161 tgctatcaaa atgtgattct gtctgatgta ctttcacaat ctctcttcaa ttccattttg
     2221 aagtgcttta aacccgaaag gttgagaaaa atgcgagcgc tcaaatattt gtattgtgtt
     2281 cgttgagtga cccaacaaaa agaggaaact ttattgtgcc gccaagaaaa aagtctcacg
     2341 tacggtgcgc gcgatgccgt atcacaactg agaaaaaaga caaaacttat tgaacagtgt
     2401 tcctttcaaa aaattatttt gtaaaaaatc gagtttttct ccaaaataac gcacgcaatc
     2461 aaaaacaaga aaactaacgt gttctcctgg gcgtggaact ccttatcaag attaccatta
     2521 ttgtctgtca gaattgatat ttcaattatt tcatatctag acaaacccgg gtgtcttgca
     2581 tttattagta ctgtgagttt acatacaaac gaggcttact cagataacat agagtcacct
     2641 ctaattagca aaaacccatt tctaattagc tatttcgacc agttctaaag tcaccgtaat
     2701 tagaaatggt ttttgtagct tttctaatta gaaatgaccc cgagataagt gaaatatggc
     2761 attttctggg ttttctagta agaaatgatc ccgcacgtag tgattccgcg ttacaagcca
     2821 aattcaaatt aaaaaagtat attacacaat tcaagttaca ttattttcag gaaacaatgg
     2881 cgaagagcaa agcagagatt caaaaggagg aaagagacaa cagatatgaa cgaagacttc
     2941 ttgaaaagag aaagagacat gaggtgagta accatgataa taaataatca agtaagggct
     3001 atagatttat aaattctgga gttaacagtt tcgaaaaaaa gtagttttca tatgtgcgaa
     3061 ggaatatcaa aaatagggga atagcctaaa cgagtgagaa aattaaatgt ttgttatttt
     3121 ttcaaaaatg tctgcagggc cgttatcctt tcaaatgcac tctatcaaac atagatcata
     3181 tccatcatat cttatccaga atttatatca tatccaagat tgagagcgtt tcaaggaaaa
     3241 tctcaaacaa ccatacaatt ttgtagatcg acaacaaggc ctccacttct tcatcgatgg
     3301 cctctgcagc acctgcacca ccaagtttgt atttttcata ttaatataaa gtgagccacg
     3361 catagtcgtt tttaggattt ttgccagatc gatgtcgcgc aaacagactg tttttttttc
     3421 tttgaaagcc gtgcgtgaca tgggccacgg attgtaacgc cgcgctggat ggataaaaat
     3481 ttattcagtt ttttagcctt tacaaaatat ggttcgacta ctttctttat ttttacattt
     3541 ttataggtgc tcattgagct gagatatttt gggtataatc ggaaaatcgc tgattttggt
     3601 catttttctg cactaaagat tacacaaaaa gttcagtatt tagattcatt gttgaaaatt
     3661 ggggatgatc tacagataca tatcgccgtg ttcaaaaaca cacgtgacgt cgtgactggg
     3721 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     3781 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     3841 aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
     3901 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     3961 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     4021 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     4081 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     4141 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     4201 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     4261 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     4321 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     4381 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     4441 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     4501 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     4561 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     4621 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     4681 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     4741 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     4801 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     4861 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     4921 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     4981 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     5041 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     5101 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     5161 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     5221 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     5281 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     5341 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     5401 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     5461 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     5521 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     5581 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     5641 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     5701 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     5761 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     5821 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     5881 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggtct
     5941 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     6001 gtattaccgc ctttgagtga gctgatacca gcggataaca atttcacagg gccctgtcgg
     6061 attatgggct cttcttttat cctctacttt ctttctagaa aatgcaaatg ttgagtcaga
     6121 aattgctgaa tggagtacaa ctgcaatggg tttagcttat gtttctggaa caattactgg
     6181 tggaattgtg attgaaaagt aaggttatat ttcatttcaa taaatgttac cacagtataa
     6241 caaaaacaag ttacattgtt cagagtatgt ctgaatgtat tattattatt gtatttgtgc
     6301 atcagtactg tagttgtact gaagaagggt ttaaaataat ctcaacgttt ttaagacgag
     6361 ttagtgagaa tacggtcgca gttaggatgt ttggacatag ttttattaga taatcgtgaa
     6421 ctaactcact ataaaaaccc gctaactcat tgaataagat agttttaaac gttcaaaatt
     6481 tgaagacaaa attgtatgaa taagtttaca gagttggtcg acgcaagctt ctccttctct
     6541 tcacatttct caataattta gctctaattg cattcgtatt cttcgcaaaa atacgtattc
     6601 tgattgaccc gatgaaatac ggatgcctag ttgcattgat catctacggt tatacttatg
     6661 ggtgaggttt ttcaaatata ttttgattta atcgaataat gaaatttcag aactggagtt
     6721 ggtccaattt cgtggtttat atcctctgaa ctggtacctc aaaaacatcg tagtattgct
     6781 caatctgtgg catattcaat gaatactgta atggtggtta tcagtacatt tacagtatta
     6841 cctctatatt ccgttattgg tagttatgct ttccttattc tatattcaat tccttcattt
     6901 ataagtatgc ttatattatt tcgctactta ccggaaacca aaggacgaga gattcatgat
     6961 attgtgaatg aacttaaaaa aaaatgaaaa cctttgaaaa cgttcatacg gccgaaattt
     7021 tacttgatcg agccaattga acttttgtta agacttacag aaaagtgaga gattttgtta
     7081 caaaaaagtg agaaaaaagc gaaaatgaac aggaaaaaga agaaaaatac acccgaagta
     7141 tcatataaaa attaaaaaga ttgtttgacc tggcggaact tttttgcaaa aactctgaat
     7201 atccatggca catgcttcgt gcaaaacagg atccaaatga atatcaacaa gagattcttg
     7261 aagacgacga gccacttgct cagagcattg cttatctcga atgagaccct tgttaaaatc
     7321 atttttcaaa cattatatga cattgtcgaa atgtcctcct gattccatga tggtagcaaa
     7381 ctcacttcgt ctttgagcca atttatccaa gtccttgtaa aaagtattcg aaaattgtta
     7441 acggataaaa tgtttattat aatatcaaaa acaatttgtc agttgaccac tttttgatat
     7501 atttgacaga aaccgggatg aattggctca aagttagggc gcccttctat tacagggttt
     7561 ctgataacaa accggttatt aactcccaac aagggatgat ttcaattcat catgctcaaa
     7621 ttgacccaaa ttaagttaca tgacaaattc atcgcccttt tcaactcttc ttggctcatc
     7681 atctgttatt ctgttcctat tctgtctgca ccctataccc ctttgcatac tctctcgtca
     7741 ttcctttctt ggatagtgtc ttcttctccc cagctctgct acttctatga cttgcgcccg
     7801 ctgcttttcc gctcgttcct ctcctctctg acgtcttctt ctgcttcttt tctccttcct
     7861 ttttcccttc tcaatcttcc tctttttccc atttctgtca atcattcgaa gaagaagaag
     7921 aagaagaccc tcattcattt catttttttt tctgtcggtg tgtgctgctc ggttaagagt
     7981 gagctctcta ttccacgtct tcttcttttt cttcttcgat tcgaatcaat cactccacca
     8041 aaacgcattc gtttttggga ttcaccccgc ggttcgcaat aggtttcttt ttcaaattat
     8101 tagcgttata aatagaaaaa tggtggagtt tcaataaaaa tgataattta caaagtgtat
     8161 tttgattaca tgtactcaaa aaggttgaaa tttttcatac cagttttccg gaaatccatc
     8221 tgatatcatt atcgtatttt tctttttaaa aatgttttca aaaaaaaaca aaatatagct
     8281 ggttatttgg caccctctaa ttaccatttt cctgtcacac cacactcttt tccttcttcc
     8341 acttctttcc gttttcagcc gcttccaacc aaaccgatat gaaagccgag caacaacgat
     8401 cgattccacc cggctcggcg accttcccgt cgcaggtgag actcagaaaa ctagagaaac
     8461 cgctcaacta actcttgata tccgatttca ttcttttctc ttttcttttt gttgaacttt
     8521 tccatatttc cagatgccac ggccaccacc aagcaccgaa caaggaatca caacggaatc
     8581 ggagcttgcg aagaaagctc aaatcactcc gaacgacgtt ttagcacttc cgggaatcac
     8641 tcaaggtatc ttgctccctt tggatttcag tttaaaacat aaatttagga ttcttatgct
     8701 ccccatctgc gaacatctat aacatcgagt tcaccaagtt ccaaatccgt gatctggaca
     8761 ctgagcaagt gcttttcgag atcgccaaac cggagaacga tcaggagaat gatgagtcgc
     8821 cacaggagtc ggcaagatac gtgcgttata gatttgctcc aaactttttg aaactcaaaa
     8881 cggtcggagc aactgtggaa ttcaaagtag gagacatccc aatccatcat ttccgaatga
     8941 tcgaacgtca cttcttcaaa gatcgccttc tgaagtgttt tgactttgaa ttcggattct
     9001 gtattccgaa ttcacgaaac aactgtgaac atatctatga gttccctcaa ctctctcaac
     9061 aactcagtga gtcattattc taaaaagtac aattcaaaag actaatctct ttttcagtgg
     9121 acgacatgat caacaatcca aacgagactc gttctgacag cttctatttc gtcgataaca
     9181 aactcgtcat gcacaacaaa gccgactact catatgatgc ataaatattt aatacaaaaa
     9241 tgttctggat aattattctg tcgaatagaa aaaaaactcc aaatgtgatt aaattccaat
     9301 aattcctgtc tagtttgttc cttccccttc cccttctcat gttcaatgca ttcctaagct
     9361 tttcagttcc ccccttgttt ctatattttt tcgctgtcct gtcacactcg ctaaaaacac
     9421 taatcacacg gaaatctgtt ttcaataaaa actccaactt taactcattt tcaatttcaa
     9481 ctgaaagatt ttttcattag agaatgtcta gaactaggcc cgggctacgt aatacgactc
     9541 acttaaggcc ttgactagag ggtaccagag ctcacctagg caggaacagc tatgaccatg
     9601 attacgccaa gctat
//
