LOCUS       sequence-137496-         695 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of Cas9 gene and inducible expression of the
            plasmid curing system for CRISPR mediated genome editing of E. 
            coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Li Y, Lin Z, Huang C, Zhang Y, Wang Z, Tang YJ, Chen T, Zhao X
  TITLE     Metabolic engineering of Escherichia coli using CRISPR-Cas9 
            meditated genome editing.
  JOURNAL   Metab Eng. 2015 Sep;31:13-21. doi: 10.1016/j.ymben.2015.06.006. Epub
            2015 Jun 30.
  PUBMED    26141150
REFERENCE   2  (bases 1 to 695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2015-09"; volume: "31:13-21. doi"; pages: " 
            10.1016/j.ymben.2015.06.006. Epub 2015 Jun 30"
COMMENT     Sequence Label: pCas9cur
FEATURES             Location/Qualifiers
     source          1..695
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(78..83,84..100,101..106)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     misc_RNA        complement(424..502)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
ORIGIN
        1 agatttcagt gcaatttatc tcttcaaatg tagcacctga agtcagcccc atacgatata
       61 agttgtaatt ctcatgtttg acagcttatc atcgataagc tttaatgcgg tagtttatca
      121 cagttaaatt gctaacgcag tcaggcaccg tgtatgaaat ctaacaatgc gctcatcgtc
      181 atcctcggca ccgtcaccct ggatgctgta ggcataggct tggttatgcc ggtactgccg
      241 ggcctcttgc gggattacga aatcatcctg tggagcttag taggtttagc aagatggcag
      301 cgcctaaatg tagaatgata aaaggattaa gagattaatt tccctaaaaa tgataaaaca
      361 agcgttttga aagcgcttgt ttttttggtt tgcagtcaga gtagaataga agtatcaaaa
      421 aaagcaccga ctcggtgcca ctttttcaag ttgataacgg actagcctta ttttaacttg
      481 ctatgctgtt ttgaatggtt ccaacaagat tattttataa cttttataac aaataatcaa
      541 ggagaaattc aaagaaattt atcagccata aaacaatact taatactata gaatgataac
      601 aaaataaact actttttaaa agaattttgt gttataatct atttattatt aagtattggg
      661 taatattttt tgaagagata ttttgaaaaa gaaaa
//
