LOCUS       sequence-137501-        9602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mKate2_PH pCFJ150 - contains C. elegans codon optimized mKate2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9602)
  AUTHORS   Heppert JK, Dickinson DJ, Pani AM, Higgins CD, Steward A, Ahringer 
            J, Kuhn JR, Goldstein B
  TITLE     Comparative assessment of fluorescent proteins for in vivo imaging 
            in an animal model system.
  JOURNAL   Mol Biol Cell. 2016 Nov 7;27(22):3385-3394. doi: 
            10.1091/mbc.E16-01-0063. Epub 2016 Jul 6.
  PUBMED    27385332
REFERENCE   2  (bases 1 to 9602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E16-01-0063"; journalName: "Mol Biol Cell"; date: 
            "2016-11-7- 7"; volume: "27"; issue: "22"; pages: "3385-3394"
COMMENT     Sequence Label: JH03
FEATURES             Location/Qualifiers
     source          1..9602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..21
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             1925..1948
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1964..1987
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      1999..2322
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(3901..3920)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4020..4042
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4080..4098)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4166..4270
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4271..4339,4340..5131)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4489..4508)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5302..5890
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5791..5810
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7526..7537)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 caactttgta tagaaaagtt gaaatatcag tttttaaaaa attaaaccat aaaacaaata
       61 atataaccca atttttacat caaaccacaa gaaaaaaata catttgggcc cacggataaa
      121 gaaattaaaa aaatacattt tttaaaggcg caccgaatta aaattcattt gggtcttacc
      181 gcgtataccg tactccgttt gtttgatcat ttttgtcagc gctggcggtt gttttttcat
      241 ttcatttctg cttcaaagac gttttctcga ataatttttc gtttattctc ttttttaaaa
      301 ttaatttcta gccgtaaatg ttataaattc acccatttaa cgcaaatttc atggtaatct
      361 catggaaaaa tgcagtttct ttgttaaaga aagcttaaat agcaaaaatt ccccgacttt
      421 ccccaaaatc ctgctcgatt ttccgttttc tcattgtatt ctctcttaat taattttatc
      481 gataatcaat tgaatgtttc agacagagaa tggtctccga gctcattaaa gaaaacatgc
      541 atatgaagct ctacatggag ggaaccgtca acaaccacca cttcaagtgc acctccgagg
      601 gagagggaaa gccatacgag ggaacccaaa ccatgcgtat caaggccgtc gagggaggac
      661 cactcccatt cgccttcgac atcctcgcca cctccttcat gtacggatcc aaggtaagtt
      721 taaacatata tatactaact aaccctgatt atttaaattt tcagaccttc atcaaccaca
      781 cccaaggaat cccagacttc ttcaagcaat ccttcccaga gggattcacc tgggagcgtg
      841 tcaccaccta cgaggacgga ggagtcctca ccgccaccca agacacctcc ctccaagacg
      901 gatgcctcat ctacaacgtc aaggtaagtt taaacagttc ggtactaact aaccatacat
      961 atttaatttt cagatccgtg gagtcaactt cccatccaac ggaccagtca tgcaaaagaa
     1021 gaccctcgga tgggaggcct ccaccgagac cctctaccca gccgacggag gacttgaggg
     1081 acgtgccgac atggccctca agctcgtcgg aggaggacac ctcatctgca acctcaaggt
     1141 aagtttaaac atgattttac taactaacta atctgattta aattttcaga ccacctaccg
     1201 ttccaagaag ccagccaaga acctcaagat gccaggagtc tactacgtcg accgtcgtct
     1261 tgagcgtatc aaggaggccg acaaggagac ctacgtcgag caacacgagg tcgccgtcgc
     1321 ccgttactgc gacctcccat ccaagctcgg acaccgtgcc ggccacggac tccaagacga
     1381 cccagacctc caagccctcc tcaagggatc ccaactcctc aaggtcaagt cctcctcctg
     1441 gcgtcgtgag cgtttctaca agctccaaga ggactgcaag gtaagtttaa acatatatat
     1501 actaactaac cctgattatt taaattttca gaccatctgg caagagtccc gtaaggtaag
     1561 tttaaacagt tcggtactaa ctaaccatac atatttaaat tttcaggtca tgcgttcccc
     1621 agagtcccaa ctcttctcca tcgaggacat ccaagaggtc cgtatgggac accgtaccga
     1681 gggactcgag aagttcgccc gtgacatccc agaggaccgt tgcttctcca tcgtcttcaa
     1741 ggtaagttta aacatgattt tactaactaa ctaatctgat ttaaattttc aggaccaacg
     1801 taacaccctc gacctcatcg ccccatcccc agccgacgcc caacactggg tccaaggact
     1861 ccgtaagatc atccaccact ccggatccat ggaccaacgt caaaagtcag gagctagcgg
     1921 agccgattac aaggatgacg atgacaagag aggatctgga tctgactaca aggacgatga
     1981 cgataagcgt taagataaat gcaaaatcct ttcaagcatt cccttcttct ctatcactct
     2041 tctttctttt tgtcaaaaaa ttctctcgct aatttatttg cttttttaat gttattattt
     2101 tatgactttt tatagtcact gaaaagtttg catctgagtg aagtgaatgc tatcaaaatg
     2161 tgattctgtc tgatgtactt tcacaatctc tcttcaattc cattttgaag tgctttaaac
     2221 ccgaaaggtt gagaaaaatg cgagcgctca aatatttgta ttgtgttcgt tgagtgaccc
     2281 aacaaaaaga ggaaacttta ttgtgccgcc aagaaaaaag tctcacgtac ggtgcgcgcg
     2341 atgccgtatc acaactgaga aaaaagacaa aacttattga acagtgttcc tttcaaaaaa
     2401 ttattttgta aaaaatcgag tttttctcca aaataacgca cgcaatcaaa aacaagaaaa
     2461 ctaacgtgtt ctcctgggcg tggaactcct tatcaagatt accattattg tctgtcagaa
     2521 ttgatatttc aattatttca tatctagaca aacccgggtg tcttgcattt attagtactg
     2581 tgagtttaca tacaaacgag gcttactcag ataacataga gtcacctcta attagcaaaa
     2641 acccatttct aattagctat ttcgaccagt tctaaagtca ccgtaattag aaatggtttt
     2701 tgtagctttt ctaattagaa atgaccccga gataagtgaa atatggcatt ttctgggttt
     2761 tctagtaaga aatgatcccg cacgtagtga ttccgcgtta caagccaaat tcaaattaaa
     2821 aaagtatatt acacaattca agttacatta ttttcaggaa acaatggcga agagcaaagc
     2881 agagattcaa aaggaggaaa gagacaacag atatgaacga agacttcttg aaaagagaaa
     2941 gagacatgag gtgagtaacc atgataataa ataatcaagt aagggctata gatttataaa
     3001 ttctggagtt aacagtttcg aaaaaaagta gttttcatat gtgcgaagga atatcaaaaa
     3061 taggggaata gcctaaacga gtgagaaaat taaatgtttg ttattttttc aaaaatgtct
     3121 gcagggccgt tatcctttca aatgcactct atcaaacata gatcatatcc atcatatctt
     3181 atccagaatt tatatcatat ccaagattga gagcgtttca aggaaaatct caaacaacca
     3241 tacaattttg tagatcgaca acaaggcctc cacttcttca tcgatggcct ctgcagcacc
     3301 tgcaccacca agtttgtatt tttcatatta atataaagtg agccacgcat agtcgttttt
     3361 aggatttttg ccagatcgat gtcgcgcaaa cagactgttt ttttttcttt gaaagccgtg
     3421 cgtgacatgg gccacggatt gtaacgccgc gctggatgga taaaaattta ttcagttttt
     3481 tagcctttac aaaatatggt tcgactactt tctttatttt tacattttta taggtgctca
     3541 ttgagctgag atattttggg tataatcgga aaatcgctga ttttggtcat ttttctgcac
     3601 taaagattac acaaaaagtt cagtatttag attcattgtt gaaaattggg gatgatctac
     3661 agatacatat cgccgtgttc aaaaacacac gtgacgtcgt gactgggaaa accctggcgt
     3721 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     3781 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat
     3841 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
     3901 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     3961 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     4021 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     4081 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     4141 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     4201 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     4261 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     4321 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     4381 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     4441 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     4501 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     4561 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     4621 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     4681 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     4741 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     4801 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     4861 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     4921 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     4981 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     5041 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     5101 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     5161 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     5221 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     5281 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     5341 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     5401 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     5461 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     5521 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     5581 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     5641 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     5701 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     5761 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     5821 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     5881 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggtctttt gctggccttt
     5941 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     6001 tgagtgagct gataccagcg gataacaatt tcacagggcc ctgtcggatt atgggctctt
     6061 cttttatcct ctactttctt tctagaaaat gcaaatgttg agtcagaaat tgctgaatgg
     6121 agtacaactg caatgggttt agcttatgtt tctggaacaa ttactggtgg aattgtgatt
     6181 gaaaagtaag gttatatttc atttcaataa atgttaccac agtataacaa aaacaagtta
     6241 cattgttcag agtatgtctg aatgtattat tattattgta tttgtgcatc agtactgtag
     6301 ttgtactgaa gaagggttta aaataatctc aacgttttta agacgagtta gtgagaatac
     6361 ggtcgcagtt aggatgtttg gacatagttt tattagataa tcgtgaacta actcactata
     6421 aaaacccgct aactcattga ataagatagt tttaaacgtt caaaatttga agacaaaatt
     6481 gtatgaataa gtttacagag ttggtcgacg caagcttctc cttctcttca catttctcaa
     6541 taatttagct ctaattgcat tcgtattctt cgcaaaaata cgtattctga ttgacccgat
     6601 gaaatacgga tgcctagttg cattgatcat ctacggttat acttatgggt gaggtttttc
     6661 aaatatattt tgatttaatc gaataatgaa atttcagaac tggagttggt ccaatttcgt
     6721 ggtttatatc ctctgaactg gtacctcaaa aacatcgtag tattgctcaa tctgtggcat
     6781 attcaatgaa tactgtaatg gtggttatca gtacatttac agtattacct ctatattccg
     6841 ttattggtag ttatgctttc cttattctat attcaattcc ttcatttata agtatgctta
     6901 tattatttcg ctacttaccg gaaaccaaag gacgagagat tcatgatatt gtgaatgaac
     6961 ttaaaaaaaa atgaaaacct ttgaaaacgt tcatacggcc gaaattttac ttgatcgagc
     7021 caattgaact tttgttaaga cttacagaaa agtgagagat tttgttacaa aaaagtgaga
     7081 aaaaagcgaa aatgaacagg aaaaagaaga aaaatacacc cgaagtatca tataaaaatt
     7141 aaaaagattg tttgacctgg cggaactttt ttgcaaaaac tctgaatatc catggcacat
     7201 gcttcgtgca aaacaggatc caaatgaata tcaacaagag attcttgaag acgacgagcc
     7261 acttgctcag agcattgctt atctcgaatg agacccttgt taaaatcatt tttcaaacat
     7321 tatatgacat tgtcgaaatg tcctcctgat tccatgatgg tagcaaactc acttcgtctt
     7381 tgagccaatt tatccaagtc cttgtaaaaa gtattcgaaa attgttaacg gataaaatgt
     7441 ttattataat atcaaaaaca atttgtcagt tgaccacttt ttgatatatt tgacagaaac
     7501 cgggatgaat tggctcaaag ttagggcgcc cttctattac agggtttctg ataacaaacc
     7561 ggttattaac tcccaacaag ggatgatttc aattcatcat gctcaaattg acccaaatta
     7621 agttacatga caaattcatc gcccttttca actcttcttg gctcatcatc tgttattctg
     7681 ttcctattct gtctgcaccc tatacccctt tgcatactct ctcgtcattc ctttcttgga
     7741 tagtgtcttc ttctccccag ctctgctact tctatgactt gcgcccgctg cttttccgct
     7801 cgttcctctc ctctctgacg tcttcttctg cttcttttct ccttcctttt tcccttctca
     7861 atcttcctct ttttcccatt tctgtcaatc attcgaagaa gaagaagaag aagaccctca
     7921 ttcatttcat ttttttttct gtcggtgtgt gctgctcggt taagagtgag ctctctattc
     7981 cacgtcttct tctttttctt cttcgattcg aatcaatcac tccaccaaaa cgcattcgtt
     8041 tttgggattc accccgcggt tcgcaatagg tttctttttc aaattattag cgttataaat
     8101 agaaaaatgg tggagtttca ataaaaatga taatttacaa agtgtatttt gattacatgt
     8161 actcaaaaag gttgaaattt ttcataccag ttttccggaa atccatctga tatcattatc
     8221 gtatttttct ttttaaaaat gttttcaaaa aaaaacaaaa tatagctggt tatttggcac
     8281 cctctaatta ccattttcct gtcacaccac actcttttcc ttcttccact tctttccgtt
     8341 ttcagccgct tccaaccaaa ccgatatgaa agccgagcaa caacgatcga ttccacccgg
     8401 ctcggcgacc ttcccgtcgc aggtgagact cagaaaacta gagaaaccgc tcaactaact
     8461 cttgatatcc gatttcattc ttttctcttt tctttttgtt gaacttttcc atatttccag
     8521 atgccacggc caccaccaag caccgaacaa ggaatcacaa cggaatcgga gcttgcgaag
     8581 aaagctcaaa tcactccgaa cgacgtttta gcacttccgg gaatcactca aggtatcttg
     8641 ctccctttgg atttcagttt aaaacataaa tttaggattc ttatgctccc catctgcgaa
     8701 catctataac atcgagttca ccaagttcca aatccgtgat ctggacactg agcaagtgct
     8761 tttcgagatc gccaaaccgg agaacgatca ggagaatgat gagtcgccac aggagtcggc
     8821 aagatacgtg cgttatagat ttgctccaaa ctttttgaaa ctcaaaacgg tcggagcaac
     8881 tgtggaattc aaagtaggag acatcccaat ccatcatttc cgaatgatcg aacgtcactt
     8941 cttcaaagat cgccttctga agtgttttga ctttgaattc ggattctgta ttccgaattc
     9001 acgaaacaac tgtgaacata tctatgagtt ccctcaactc tctcaacaac tcagtgagtc
     9061 attattctaa aaagtacaat tcaaaagact aatctctttt tcagtggacg acatgatcaa
     9121 caatccaaac gagactcgtt ctgacagctt ctatttcgtc gataacaaac tcgtcatgca
     9181 caacaaagcc gactactcat atgatgcata aatatttaat acaaaaatgt tctggataat
     9241 tattctgtcg aatagaaaaa aaactccaaa tgtgattaaa ttccaataat tcctgtctag
     9301 tttgttcctt ccccttcccc ttctcatgtt caatgcattc ctaagctttt cagttccccc
     9361 cttgtttcta tattttttcg ctgtcctgtc acactcgcta aaaacactaa tcacacggaa
     9421 atctgttttc aataaaaact ccaactttaa ctcattttca atttcaactg aaagattttt
     9481 tcattagaga atgtctagaa ctaggcccgg gctacgtaat acgactcact taaggccttg
     9541 actagagggt accagagctc acctaggcag gaacagctat gaccatgatt acgccaagct
     9601 at
//
