LOCUS       sequence-137504-         589 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of Cas9 gene and inducible expression of the
            plasmid curing system for CRISPR mediated genome editing of E. 
            coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Li Y, Lin Z, Huang C, Zhang Y, Wang Z, Tang YJ, Chen T, Zhao X
  TITLE     Metabolic engineering of Escherichia coli using CRISPR-Cas9 
            meditated genome editing.
  JOURNAL   Metab Eng. 2015 Sep;31:13-21. doi: 10.1016/j.ymben.2015.06.006. Epub
            2015 Jun 30.
  PUBMED    26141150
REFERENCE   2  (bases 1 to 589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2015-09"; volume: "31:13-21. doi"; pages: " 
            10.1016/j.ymben.2015.06.006. Epub 2015 Jun 30"
COMMENT     Sequence Label: pCas9cur
FEATURES             Location/Qualifiers
     source          1..589
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   149..184
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     misc_feature    complement(185..316)
                     /label=crRNA leader
                     /note="crRNA leader sequence for the Streptococcus pyogenes
                     CRISPR/Cas system"
ORIGIN
        1 gatcatcgtg cgggagggca agggctccaa ggatcgggcg cgaaaaaacc ccgccctgtc
       61 aggggcgggg ttttttgcgg agcttagtaa agccctcgct agattttaat gcggatgttg
      121 cgattacttc gccaactatt gcgctgcagt tttgggacca ttcaaaacag catagctcta
      181 aaacctcgta gactattttt gtctaaaaaa tttcgtaatc gcactatttg tctcagctag
      241 acttcagtct tgaaaagccc ctgtattact gcatttatta agagtattat accatatttt
      301 tagttattaa gaaataatct tcatctaaaa tatacttcag tcacctccta gctgactcaa
      361 atcaatgcgt gtttcataaa gaccagtgat ggattgatgg ataagagtgg catctaaaac
      421 ttcttttgta gacgtatatc gtttacgatc aattgttgta tcaaaatatt taaaagcagc
      481 gggagctcca agattcgtca acgtaaataa atgaataata ttttctgctt gttcacgtat
      541 tggtttgtct ctatgtttgt tatatgcact aagaacttta tctaaattg
//
