LOCUS       sequence-137509-         668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of Cas9 gene and inducible expression of the
            plasmid curing system for CRISPR mediated genome editing of E. 
            coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Li Y, Lin Z, Huang C, Zhang Y, Wang Z, Tang YJ, Chen T, Zhao X
  TITLE     Metabolic engineering of Escherichia coli using CRISPR-Cas9 
            meditated genome editing.
  JOURNAL   Metab Eng. 2015 Sep;31:13-21. doi: 10.1016/j.ymben.2015.06.006. Epub
            2015 Jun 30.
  PUBMED    26141150
REFERENCE   2  (bases 1 to 668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2015-09"; volume: "31:13-21. doi"; pages: " 
            10.1016/j.ymben.2015.06.006. Epub 2015 Jun 30"
COMMENT     Sequence Label: pCas9cur
FEATURES             Location/Qualifiers
     source          1..668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   368..403
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     primer_bind     complement(608..627)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc
       61 gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag
      121 aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga
      181 atggacgatc acactactct tcttttgcct attataacat gaaaacgctt taacagaaag
      241 aataggaagg tatccgactg ctggtattaa ccctctttct caagttatca tcggcaatgt
      301 tgaatggagt ccattcaaaa cagcatagct ctaaaacatg gaactcaaca agtctcagtg
      361 tgctgaagtt ttgggaccat tcaaaacagc atagctctaa aactcgaggt cattcagact
      421 ggctaatgca cccagtaagg cagcggtatc atcaacgggg tctggtgcca ctttttcaag
      481 ttgataacgg actagcctta ttttaacttg ctatttctag ctctaaaacg cttggagcga
      541 acgacctaca tggagaaaca gtagagagtt gcgataaaaa gcgtcaggta ggatccgcta
      601 atcttatgga taaaaatgct atggcatagc aaagtgtgac gccgtgcaaa taatcaatgt
      661 ggactttt
//
