LOCUS       sequence-137515-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Allows expression of rabbit alpha-skeletal tropomyosin from a 
            rhamnose inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Eastwood T, Baker K, Brooker H, Frank S, Mulvihill DP
  TITLE     An enhanced recombinant amino-terminal acetylation system and novel 
            in vivo high-throughput screen for molecules affecting 
            alpha-synuclein oligomerisation.
  JOURNAL   FEBS Lett. 2017 Feb 18. doi: 10.1002/1873-3468.12597.
  PUBMED    28214355
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2017 Feb 18. doi: 10.1002/1873-3468.12597."
COMMENT     Sequence Label: pETpRha-alpha-SkTm
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..155
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     RBS             173..195
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatctcc acaattcagc aaattgtgaa
       61 catcatcacg ttcatctttc cctggttgcc aatggcccat tttcctgtca gtaacgagaa
      121 ggtcgcgaat tcaggcgctt tttagactgg tcgtaatgaa tctagaaata attttgttta
      181 actttaagaa ggagatatac atatggacgc catcaagaag aagatgcaga tgctgaagct
      241 cgacaaagag aacgccttgg atcgagcaga gcaggcggag gctgacaaga aggctgcgga
      301 agaccggagc aagcagctgg aagatgagct ggtgtcactg caaaagaaac tcaagggcac
      361 tgaagatgaa ctggacaaat actccgaggc tctcaaagat gcccaggaga aactggagct
      421 ggcggagaaa aaggccacag atgctgaagc tgacgtagca tctctgaaca gacgcatcca
      481 gctggttgag gaggagttgg atcgcgctca ggagcgtctg gccacagctc tacagaagct
      541 ggaggaggct gagaaggctg cagatgagag tgagagaggc atgaaagtca ttgaaagccg
      601 agcccaaaaa gatgaagaaa agatggagat tcaggagatc cagctgaaag aggccaagca
      661 cattgctgaa gatgctgacc gaaagtatga agaggtggcc cgtaagctgg tcatcatcga
      721 gagcgatctg gagcgtgcgg aggagagggc tgagctctcg gaaggcaaat gtgccgagct
      781 tgaagaagag ttgaaaacgg tgacgaacaa cttgaagtca ctggaggctc agg
//
