LOCUS       sequence-13752-e       10672 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10672)
  AUTHORS   Moore AT, Rankin KE, von Dassow G, Peris L, Wagenbach M, Ovechkina 
            Y, Andrieux A, Job D, Wordeman L
  TITLE     MCAK associates with the tips of polymerizing microtubules.
  JOURNAL   J Cell Biol. 2005 May 9. 169(3):391-7.
  PUBMED    15883193
REFERENCE   2  (bases 1 to 10672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2005 May 9. 169(3):391-7."
COMMENT     Sequence Label: GFP-Tog
FEATURES             Location/Qualifiers
     source          1..10672
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             complement(1444..1461)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      6612..6621
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6618..6620,6621..6623,6624..7337)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6618..6620,6621..6623,6624..7334)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6663..6684)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6924..6943)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7271..7292
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7460..7581
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7497..7516)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7551..7570
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7588..8043)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7725..7746)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7937..7956
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8070..8174
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8176..8533
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8176..8367
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8199..8219)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8384..8519
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8568..9362
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8568..9362
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8622..8641)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9232..9251
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9550..9569)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9594..9641
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9970..10558
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10459..10478
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcggaattct
      661 aaggaaaacc tggaagcaca atgggagatg acagtgagtg gttgaaactg ccagttgatc
      721 agaaatgtga acacaagctg tggaaagcaa ggttaagtgg gtatgaagag gccctgaaga
      781 tcttccagaa aataaaggat gaaaagagcc cagagtggtc caaattttta ggattgatca
      841 aaaaatttgt cactgattcc aatgcagtgg ttcaattgaa aggattagaa gctgcacttg
      901 tttatgttga aaatgcccat gtagcaggaa aaaccacagg agaagttgtg tcaggtgttg
      961 taagtaaggt gttcaatcaa cctaaagcta aagccaagga gctgggcata gagatctgtc
     1021 ttatgtacat agagattgag aaaggagagg ctgttcaaga agagctcctg aaaggcttgg
     1081 acaataagaa tcccaagatc atagtggcct gtatagagac actgaggaaa gccttaagtg
     1141 aatttggttc caaaatcatc ttgcttaagc caattatcaa agtgttgcca aaactctttg
     1201 agtctcgaga gaaggctgtt cgagatgaag ccaaactaat tgctgtggag atttacagat
     1261 ggattcggga tgctctgaga cccccattac aaaatataaa ctctgttcag ttgaaagaac
     1321 tagaagaaga atgggtcaaa ctgccaacaa gtgctcctag acctactcga tttcttcgtt
     1381 cccaacaaga actagaagct aaattggaac aacaacagtc tgctggtgga gatgctgaag
     1441 gaggtggtga tgatggtgat gaggtgccac aaatagatgc ttatgagctt ttagaagctg
     1501 tagaaatcct ttccaaactt cccaaagact tttatgacaa aattgaggca aaaaaatggc
     1561 aagagagaaa agaggccctg gagtctgtag aagtactaat aaaaaacccc aaactggaag
     1621 ctggcgatta tgcagattta gtaaaagcat taaagaaggt tgttggaaag gacaccaatg
     1681 tcatgttggt ggctttggca gcaaaatgtc ttactggcct ggctgttggg ctaaggaaga
     1741 aatttggaca atatgcagga catgttgtgc caaccatctt ggagaaattc aaagagaaga
     1801 aacctcaagt ggtacaagcc ctgcaggagg caattgatgc aatcttcctt actaccacac
     1861 tacagaacat cagtgaggat gttttagcag taatggataa taaaaatcca accatcaagc
     1921 agcagacatc tctttttatt gcaagaagtt tccgccactg cactgcttct accctgccaa
     1981 agagcttgct aaagcccttt tgtgctgcac tacttaagca catcaatgat tctgctcctg
     2041 aagtcagaga tgccgcattt gaagcattgg gtactgcttt gaaggtggtt ggcgagaaag
     2101 cagtaaaacc attcctagct gatgtggaca aactcaagct tgataagatc aaagaatgtt
     2161 cagaaaaggt agaactgata catggtaaga aagctggact agctgctgat aagaaggaat
     2221 tcaaacctct gcctggaagg actgctgctt caggggctgc aggagataag gacacaaagg
     2281 acatttctgc acccaaacca ggacctctaa aaaaggcacc tgctgctaag gctggtgggc
     2341 caccaaaaaa ggggaaacca gctgcaccag gaggcgcagg gaatactgga accaagaaca
     2401 agaaaggact ggagactaaa gaaatagtgg agcctgagct ctcgatagaa gtatgcgaag
     2461 aaaaagcttc agctgttctt ccccctacct gtatacagct tcttgacagc agtaactgga
     2521 aagaaaggct ggcttgtatg gaagagttcc agaaggctgt tgagctaatg gaccgaactg
     2581 aaatgccatg ccaggcatta gtgaggatgc tagccaagaa acctggatgg aaagaaacta
     2641 attttcaggt gatgcaaatg aagcttcata tagttgcttt gattgcccag aagggaaatt
     2701 tttccaaaac gtcagctcag gttgtattag atggccttgt ggacaagatt ggagatgtga
     2761 aatgtgggaa caatgcaaaa gaagctatga cagcaatagc cgaagcctgt atgttaccat
     2821 ggactgctga acaggttgtg tcaatggctt tctcacaaaa gaatcccaaa aatcagtcag
     2881 aaactctgaa ttggctatca aatgccataa aagaatttgg tttttctggg ttgaatgtca
     2941 aagctttcat tagcaatgtg aagacagctc ttgctgcaac aaacccagct gtgaggactg
     3001 ctgccataac cctgcttggc gtgatgtatc tgtatgttgg tccctctttg cgaatgttct
     3061 ttgaggatga gaagcctgcc ctcctatccc agatagatgc agaatttgag aagatgcagg
     3121 gacaaagtcc acctgctcca accagaggaa tttccaagca tagcacaagt ggtacagatg
     3181 aaggagaaga tggagatgaa ccagatgacg ggagcaatga tgtcgttgat cttttgccga
     3241 ggacggagat cagtgataaa atcacttcag agttggtatc taagattggt gataagaatt
     3301 ggaagattag gaaagaaggc ctagatgaag tggcaggtat tattaatgac gcaaaattta
     3361 tccaaccgaa tataggtgaa cttccaactg ccttgaaggg tcgactcaat gattcaaata
     3421 aaatcttggt acagcaaacg ctgaatatcc tgcaacaact ggcagtagcc atgggcccaa
     3481 atattaagca acatgtaaaa aatttaggca tccctatcat cacagtcctt ggagacagca
     3541 agaacaatgt tcgagctgct gccctagcga ctgtgaatgc ttgggcagaa cagactggca
     3601 tgaaggaatg gctggaagga gaagatcttt ctgaagagct caaaaaggaa aatcctttct
     3661 tgaggcaaga gcttctgggc tggctggctg agaaactacc tactcttcgt tccaccccta
     3721 cagaccttat cctttgtgtt cctcatctct actcctgcct agaagatcga aatggagatg
     3781 tgcgaaagaa ggcccaagat gccttgccat tcttcatgat gcatttagga tatgaaaaaa
     3841 tggccaaggc tactgggaaa ctaaagccaa cttctaaaga tcaggtattg gccatgctag
     3901 agaaagccaa agttaacatg ccagccaagc ctgctccacc cactaaagca acttctaaac
     3961 caatgggagg gtccgctcca gccaaattcc agcctgcatc agcacctgct gaagattgta
     4021 tttccagcag tacagaaccc aaacctgatc caaaaaaggc caaagctcca ggattatcct
     4081 ctaaagcaaa gagtgcacaa gggaagaaga tgccaagcaa aaccagctta aaggaggatg
     4141 aagacaaatc cgggcctatt tttattgttg ttccaaatgg aaaagagcaa aggatgaaag
     4201 atgaaaaagg attgaaggtg ctaaagtgga attttactac cccacgggat gaatacattg
     4261 agcaactaaa gactcaaatg tctagctgtg tggctaaatg gttacaagat gagatgtttc
     4321 actcagactt tcagcatcat aacaaagccc ttgctgttat ggttgatcac ttggagagtg
     4381 aaaaagaagg agttattggt tgcctggatc ttatcttaaa gtggcttacc ctgaggtttt
     4441 ttgacaccaa tacaagcgtc ctgatgaaag cactagaata tttaaaattg ctcttcacct
     4501 tgctaagtga agaagaatat catcttactg agaatgaagc atcttccttc atcccctatc
     4561 ttgtcgtcaa ggttggagaa ccaaaggatg tcattcgtaa agatgttcgt gccatcctga
     4621 accggatgtg ccttgtctac ccagctagca agatgtttcc ctttatcatg gaaggaacca
     4681 aatccaaaaa ctctaagcag agagcagagt gcctggaaga gctgggatgt ctggttgagt
     4741 cctatggcat gaatgtttgc caaccaaccc caggaaaagc cttaaaggaa atagctgttc
     4801 acataggaga ccgtgacaat gctgtacgca atgctgcact caacaccatt gtaacggtgt
     4861 acaatgtaca tggggatcag gtgttcaaac tgattggaaa tctttctgaa aaggatatga
     4921 gcatgctcga ggagaggatt aagcggtcag caaagagacc ctctgctgca ccaataaaac
     4981 aggtggaaga gaaacctcag cgtgcacaga acataagctc caatgccaac atgttacgca
     5041 agggaccagc tgaggacatg tcttccaaac tcaaccaagc ccgaagcatg agtgggcatc
     5101 ctgaggcagc ccagatggtc cgccgagaat tccagctgga tctagatgag attgagaatg
     5161 acaatggtac agtccgatgt gaaatgccag aacttgttca gcacaaactg gatgacattt
     5221 ttgagccagt ccttattcct gaacccaaga tccgggctgt ttctccacac ttcgatgaca
     5281 tgcacagtaa tacagcatcc acaatcaatt tcattatctc ccaagtagcc agtggtgaca
     5341 tcaacacaag tatccaagct ctgacacagc tgtttcagat agagagcctt gcccgggagg
     5401 cctccactgg agtactaaaa gacctaatgc atggcctcat caccttaatg ctggattctc
     5461 ggattgaaga tcttgaggaa ggacaacagg tcatccgctc tgtgaacctc ttggtggtga
     5521 aggttctgga gaagtcagac cagaccaaca tcctgagtgc cctacttgtt ttgctccaag
     5581 acagcctgct agcaacagcc agttctccca aattctcaga gcttgttatg aagtgtctct
     5641 ggagaatggt tcgactgttg cctgatacca tcaatagcat taacctagac agaattcttc
     5701 tggatatcca cattttcatg aaggtcttcc ccaaagagaa actgaagcaa tgcaaaagtg
     5761 aatttcccat aaggacccta aagaccctgc tacacacctt atgcaaatta aaagggccca
     5821 agatcctgga ccacctaacg atgatcgaca acaaaaacga gtctgagctg gaggcccatc
     5881 tctgccggat gatgaagcac agtatggacc agactgggag caagtctgat aaggaaacag
     5941 caaagggagc atctcgaata gatgcaaaat catcaaaggc caaagtgaat gatttcttag
     6001 ctgagatttt taagaagatt ggctctaaag aaaacactaa agagggacta gcagagttat
     6061 atgaatataa gaagaaatac tcagatgctg acattgaacc atttctgaaa aattcctcac
     6121 agttcttcca gagctatgtc gaaagaggcc ttcgggtgat tgagatggag agggagggca
     6181 aaggtcgtat ttccacttca acaggcatct cccctcagat ggaagtcaca tgtgtgccca
     6241 cgcccacaag cacagtgtcc tccataggta acacaaatgg ggaagaagtg gggccatctg
     6301 tctacttgga aaggctaaag atcctccgac agcgatgtgg tctggacaac acaaagcaag
     6361 atgaccgacc tcctttgacc tctttgctct ccaaaccagc agttcctact gtcgcctctt
     6421 ccacagacat gctccacagc aaactctctc agctccggga gtcacgggag cagcaccagc
     6481 attcagacct ggattctaac cagactcact cttcaggaac tgtgacctcc tcctcctcca
     6541 cagctaacat agacgacttg aaaaaaagac tggagagaat aaagagcagt cgcaaagcgg
     6601 atccaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     6661 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     6721 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     6781 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     6841 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     6901 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     6961 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     7021 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     7081 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     7141 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     7201 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     7261 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     7321 acgagctgta caagtaaagc ggccgcgact ctagatcata atcagccata ccacatttgt
     7381 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     7441 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     7501 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     7561 caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg
     7621 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     7681 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     7741 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     7801 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     7861 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     7921 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     7981 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     8041 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     8101 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     8161 gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
     8221 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     8281 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     8341 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     8401 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     8461 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     8521 aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     8581 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     8641 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     8701 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag
     8761 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     8821 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     8881 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     8941 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     9001 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     9061 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg
     9121 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     9181 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     9241 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     9301 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     9361 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     9421 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     9481 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg
     9541 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat
     9601 aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc
     9661 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt
     9721 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa
     9781 cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt
     9841 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     9901 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     9961 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
    10021 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
    10081 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
    10141 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
    10201 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
    10261 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
    10321 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
    10381 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
    10441 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    10501 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
    10561 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
    10621 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc at
//
