LOCUS       sequence-137523-         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses S. pyogenes CRISPR chimeric RNA element with customizable 
            sgRNA from U6 promoter and Crimson reporter from EF-1a promoter. 
            Lentiviral backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Canver MC, Smith EC, Sher F, Pinello L, Sanjana NE, Shalem O, Chen 
            DD, Schupp PG, Vinjamur DS, Garcia SP, Luc S, Kurita R, Nakamura Y, 
            Fujiwara Y, Maeda T, Yuan GC, Zhang F, Orkin SH, Bauer DE
  TITLE     BCL11A enhancer dissection by Cas9-mediated in situ saturating 
            mutagenesis.
  JOURNAL   Nature. 2015 Sep 16. doi: 10.1038/nature15521.
  PUBMED    26375006
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Sep 16. doi: 10.1038/nature15521."
COMMENT     Sequence Label: lentiGuide-Crimson
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    284..401
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        complement(457..532)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 aaaggcggag ccagtacacg acatcacttt cccagtttac cccgcgccac cttctctagg
       61 caccggatca attgccgacc cctcccccca acttctcggg gactgtgggc gatgtgcgct
      121 ctgcccactg acgggcaccg gagccaattc ccactccttt caagacctag aaggtccatt
      181 agctgcaaag attcctctct gtttaaaact ttatccatct ttgcacccgg gccccctcga
      241 gccggcgcca aagtggatct ctgctgtccc tgtaataaac ccgaaaattt tgaatttttg
      301 taatttgttt ttgtaattct ttagtttgta tgtctgttgc tattatgtct actattcttt
      361 cccctgcact gtacccccca atcccccctt ttcttttaaa attgtggatg aatactgcca
      421 tttgtctcaa gatctagtta cgccaagctt aaaaaagcac cgactcggtg ccactttttc
      481 aagttgataa cggactagcc ttattttaac ttgctatttc tagctctaaa acagagacgt
      541 acaaaaaaga gcaagaagct aaaaaagatt taaaaattat ttttagcgca gttaatggaa
      601 caggaactaa atttacccca aaaatattac gtgaatcagg atataacgtt attgaggttg
      661 aagagcatgc atttgaagat gaaacattta aaaatgttgt aaatccaaat ccagaatttg
      721 atcctgcatg aaaaataccg cttgaatatg gtattaaaca tgatgcagat attattatta
      781 tgaatgaccc agatgctgac agatttggaa tggcaataaa acatgatggt cattttgtaa
      841 gattagatgg aaatcaaaca ggaccaattt taattgattg aaaattatca aatctaaaac
      901 gcttaaatag cattccaaaa
//
