LOCUS       sequence-137527-         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  receiving vector of standard transcription units(TUs) in YeastFab 
            work, useful for further assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: POT3-RFP
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    192..213
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(228..233,234..251,252..258)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    266..282
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             300..311
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 agctggagct ccaccgcggt ggcggccgcg aattccccgg gtctagaggt ctcggttggc
       61 agtgactcgg tctctaggcg gctagagacg gcaatacgca aaccgcctct agccgcgcgt
      121 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
      181 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
      241 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac atactagaga
      301 aagaggagaa atactagatg gcttcctccg aagacgttat caaagagttc atgcgtttca
      361 aagttcgtat ggaaggttcc gttaacggtc acgagttcga aatcgaaggt gaaggtgaag
      421 gtcgtccgta cgaaggtacc cagaccgcta aactgaaagt taccaaaggt ggtccgctgc
      481 cgttcgcttg ggacatcctg tccccgcagt tccagtacgg ttccaaagct tacgttaaac
      541 acccggctga catcccggac tacctgaaac tgtccttccc ggaaggtttc aaatgggaac
      601 gtgttatgaa cttcgaagac ggtggtgttg ttaccgttac ccaggactcc tccctgcaag
      661 acggtgagtt catctacaaa gttaaactgc gtggtaccaa cttcccgtcc gacggtccgg
      721 ttatgcagaa
//
