LOCUS       sequence-137535-        2607 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  standard plasmids for exogenus pathway assembly haboring 
            recombination sequence 2 (Universal Recombination Region 2) which 
            could be released and ligated to series of TUs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: pMV-URR2
FEATURES             Location/Qualifiers
     source          1..2607
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     94..112
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     121..138
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     122..138
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(710..726)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(710..726)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(738..755)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(909..1497)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(989..1008)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1668..2459,2460..2528))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2291..2310
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(2529..2607,1..26))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
       61 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
      121 tgtaaaacga cggccagtcg aaccacgcaa tgcgtctcga tccgcagtgt cttgcgtctc
      181 tcgctagtaa catcagctaa cgaaagagtt agaggctcgc taaatcgcac tgtcggggtc
      241 ccttgggtat tttacactag cgtcaggacg actagcatgt gtctttcctt ccaggggtat
      301 gcgggtgcgt ggacaaatga gcagcatacg tatttactcg gcgtgcctgc tctctcgtat
      361 ttctcctgga gatcaaggaa atgtttcatg tccaagcgaa aagccgctct acggaatgga
      421 tctacgttac tgcctgcata aggaaaccgg tgtagccaag gacgaaagcg accctaggtt
      481 ctaaccatcg actttggcgg aaaggtttca ctcaggaagc agacactgat tgacacggtt
      541 tagcagaacg tttgaggact aggtcaaatt gagtggttta atatcggcat gtctggcttt
      601 aaaattcagt atagtgcgct gatcggaaac gaattaaaaa cacgagttcc caaaaccagg
      661 cgggctcgcc acgctaatcg gagagacgga gtcactgcca accgagacgg tcatagctgt
      721 ttcctgtgtg ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
      781 gcggtatcag ctcactcaaa ggcggtaata cggttaccca cagaatcagg ggataacgca
      841 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      901 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      961 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1021 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     1081 tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc
     1141 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     1201 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     1261 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     1321 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     1381 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1441 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1501 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     1561 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     1621 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     1681 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     1741 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaata
     1801 ataccgcggg acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     1861 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     1921 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatc
     1981 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     2041 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     2101 ggtcctccga tcgttgtcag aagtaagttg gccgccgtgt tatcactcat ggttatggca
     2161 gcactacata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     2221 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     2281 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     2341 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     2401 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     2461 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     2521 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     2581 agcggataca tatttgaatg tatttag
//
